Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii

Detalhes bibliográficos
Autor(a) principal: Teixeira, Pedro Antônio Castelo
Data de Publicação: 2009
Outros Autores: Castro, Rafaela Alves de, Nascimento, Rosana Cícera, Tronchin, Guy, Pérez Torres, Armando, Lazéra, Márcia, Almeida, Sandro Rogério de, Bouchara, Jean-Philippe, Loureiro y Penha, Carla Veronica, Lopes-Bezerra, Leila M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38996
Resumo: 2021-01-06
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spelling Teixeira, Pedro Antônio CasteloCastro, Rafaela Alves deNascimento, Rosana CíceraTronchin, GuyPérez Torres, ArmandoLazéra, MárciaAlmeida, Sandro Rogério deBouchara, Jean-PhilippeLoureiro y Penha, Carla VeronicaLopes-Bezerra, Leila M.2020-01-06T15:23:39Z2020-01-06T15:23:39Z2009TEIXEIRA, Pedro Antônio Castelo et al. Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii. Microbiology, v. 155, n. 11, p. 3730-3738, 1 Nov. 2009.1350-0872https://www.arca.fiocruz.br/handle/icict/3899610.1099/mic.0.029439-01465-2080engMicrobiology SocietyCell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-06Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil / Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Université d’Angers. Groupe d’Étude des Interactions Hôte-Pathogène. Angers, France.Universidad Nacional Autónoma de México. Facultad de Medicina Laboratorio de Inmunología Comparada de Piel y Mucosas. Mexico, DF, Mexico.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Université d’Angers. Groupe d’Étude des Interactions Hôte-Pathogène. Angers, France / Centre Hospitalier Universitaire. Laboratoire de Parasitologie-Mycologie. Angers, France.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.The virulence of four Sporothrix schenckii isolates was compared in a murine model of sporotrichosis, together with the protein pattern of the yeast cell surface and the capacity to bind the extracellular matrix protein fibronectin. Virulence was determined by the mortality rate, fungal burden and histopathology. Two clinical isolates were more virulent for C57BL/6 mice, but no direct correlation was seen between virulence and the clinical or environmental origin of the isolates. The lowest virulence was observed for an isolate recovered from a patient with meningeal sporotrichosis. Although all isolates could effectively disseminate, the dissemination patterns were not similar. Using flow cytometry analysis, we investigated the interaction of all the strains with fibronectin, and showed that the binding capacity correlated with virulence. Western blot analysis of S. schenckii cell wall extracts revealed positive bands for fibronectin in the range of 37–92 kDa. The 70 kDa adhesin was also recognized by a protective monoclonal antibody raised against a gp70 antigen of S. schenckii (mAb P6E7). Confocal microscopy confirmed the colocalization of fibronectin and mAb P6E7 on the yeast cell surface. To our knowledge, this is the first report identifying adhesins for fibronectin on the surface of this human pathogen.Sporothrix schenckiiVirulenceFibronectininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/38996/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Teixeira_Pedro_etal_INI_2009.pdfve_Teixeira_Pedro_etal_INI_2009.pdfapplication/pdf740535https://www.arca.fiocruz.br/bitstream/icict/38996/2/ve_Teixeira_Pedro_etal_INI_2009.pdf97d70c59ea373c440c21a71ebba6a06eMD52TEXTve_Teixeira_Pedro_etal_INI_2009.pdf.txtve_Teixeira_Pedro_etal_INI_2009.pdf.txtExtracted texttext/plain38275https://www.arca.fiocruz.br/bitstream/icict/38996/3/ve_Teixeira_Pedro_etal_INI_2009.pdf.txt942088bae49d04724b19eb9e157e21c5MD53icict/389962021-04-27 14:09:56.374oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-04-27T17:09:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en.fl_str_mv Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
title Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
spellingShingle Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
Teixeira, Pedro Antônio Castelo
Sporothrix schenckii
Virulence
Fibronectin
title_short Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
title_full Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
title_fullStr Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
title_full_unstemmed Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
title_sort Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii
author Teixeira, Pedro Antônio Castelo
author_facet Teixeira, Pedro Antônio Castelo
Castro, Rafaela Alves de
Nascimento, Rosana Cícera
Tronchin, Guy
Pérez Torres, Armando
Lazéra, Márcia
Almeida, Sandro Rogério de
Bouchara, Jean-Philippe
Loureiro y Penha, Carla Veronica
Lopes-Bezerra, Leila M.
author_role author
author2 Castro, Rafaela Alves de
Nascimento, Rosana Cícera
Tronchin, Guy
Pérez Torres, Armando
Lazéra, Márcia
Almeida, Sandro Rogério de
Bouchara, Jean-Philippe
Loureiro y Penha, Carla Veronica
Lopes-Bezerra, Leila M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Pedro Antônio Castelo
Castro, Rafaela Alves de
Nascimento, Rosana Cícera
Tronchin, Guy
Pérez Torres, Armando
Lazéra, Márcia
Almeida, Sandro Rogério de
Bouchara, Jean-Philippe
Loureiro y Penha, Carla Veronica
Lopes-Bezerra, Leila M.
dc.subject.en.en.fl_str_mv Sporothrix schenckii
Virulence
Fibronectin
topic Sporothrix schenckii
Virulence
Fibronectin
description 2021-01-06
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-01-06T15:23:39Z
dc.date.available.fl_str_mv 2020-01-06T15:23:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, Pedro Antônio Castelo et al. Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii. Microbiology, v. 155, n. 11, p. 3730-3738, 1 Nov. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38996
dc.identifier.issn.pt_BR.fl_str_mv 1350-0872
dc.identifier.doi.none.fl_str_mv 10.1099/mic.0.029439-0
dc.identifier.eissn.pt_BR.fl_str_mv 1465-2080
identifier_str_mv TEIXEIRA, Pedro Antônio Castelo et al. Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii. Microbiology, v. 155, n. 11, p. 3730-3738, 1 Nov. 2009.
1350-0872
10.1099/mic.0.029439-0
1465-2080
url https://www.arca.fiocruz.br/handle/icict/38996
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Microbiology Society
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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