A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host

Detalhes bibliográficos
Autor(a) principal: Bernardes, Wilma Patrícia de Oliveira Santos
Data de Publicação: 2019
Outros Autores: Araújo, Juliano Michel de, Carvalho, Gardênia Braz, Alves, Clarice Carvalho, Coelho, Aline Thaynara de Moura, Dutra, Isabela Thamara Sabino, Teixeira, Sueleny Silva Ferreira, Ribeiro, Rosy Iara Maciel de Azambuja, Mourão, Marina de Moraes, Pereira, Rosiane Aparecida da Silva, Fonseca, Cristina Toscano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40002
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.
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spelling Bernardes, Wilma Patrícia de Oliveira SantosAraújo, Juliano Michel deCarvalho, Gardênia BrazAlves, Clarice CarvalhoCoelho, Aline Thaynara de MouraDutra, Isabela Thamara SabinoTeixeira, Sueleny Silva FerreiraRibeiro, Rosy Iara Maciel de AzambujaMourão, Marina de MoraesPereira, Rosiane Aparecida da SilvaFonseca, Cristina Toscano2020-02-17T13:17:58Z2020-02-17T13:17:58Z2019BERNARDES, Wilma Patrícia de Oliveira Santos et al. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research, v. 2019, p. 1-16, 2019.2314-8861https://www.arca.fiocruz.br/handle/icict/4000210.1155/2019/6793596engHindawiA Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Hostinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.Universidade Federal de São João Del Rei. Laboratório de Patologia Experimental. Divinópolis, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Diagnóstico e Terapia de Doenças Infecciosas e Oncológicas. Belo Horizonte, MG, Brasil.Universidade Federal de São João Del Rei. Laboratório de Patologia Experimental. Divinópolis, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Helmintologia e Malacologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.Sm16 is an immunomodulatory protein that seems to play a key role in the suppression of the cutaneous inflammatory response duringSchistosoma mansonipenetration of the skin of definitive hosts. Therefore, Sm16 represents a potential target for protective immuneresponses induced by vaccination. In this work, we generated the recombinant protein rSm16 and produced polyclonal antibodies against this protein to evaluate its expression during different parasite life-cycle stages and its location on the surface of the parasite.In addition, we analyzed the immune responses elicited by immunization with rSm16 using two different vaccine formulations, aswell as its ability to induce protection in Balb/c mice. In order to explore the biological function of Sm16 during the course ofexperimental infection, RNA interference was also employed. Our results demonstrated that Sm16 is expressed in cercaria and schistosomula and is located in the schistosomula surface. Despite humoral and cellular immune responses triggered by vaccination using rSm16 associated with either Freund’s or alum adjuvants, immunized mice presented no reduction in either parasite burdenor parasite egg laying. Knockdown of Sm16gene expression in schistosomula resulted in decreased parasite sizein vitrobut had noeffect on parasite survival or egg productionin vivo. Thus, ourfindings demonstrate that although the vaccine formulations used inthis study succeeded in activating immune responses, these failed to promote parasite elimination. Finally, we have shown that Sm16 is not vital for parasite survival in the definitive host and hence may not represent a suitable target for vaccine development.Schistosoma mansoniVaccineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/40002/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALSm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host.pdfSm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host.pdfapplication/pdf18513912https://www.arca.fiocruz.br/bitstream/icict/40002/2/Sm16%2c%20A%20Schistosoma%20mansoni%20Immunomodulatory%20Protein%2c%20Fails%20to%20Elicit%20a%20Protective%20Immune%20Response%20and%20Does%20Not%20Have%20an%20Essential%20Role%20in%20Parasite%20Survival%20in%20the%20Definitive%20Host.pdfb16a4c95b374bfe5e9e81e84d5297ff4MD52TEXTSm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host.pdf.txtSm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
title A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
spellingShingle A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
Bernardes, Wilma Patrícia de Oliveira Santos
Schistosoma mansoni
Vaccine
title_short A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
title_full A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
title_fullStr A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
title_full_unstemmed A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
title_sort A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
author Bernardes, Wilma Patrícia de Oliveira Santos
author_facet Bernardes, Wilma Patrícia de Oliveira Santos
Araújo, Juliano Michel de
Carvalho, Gardênia Braz
Alves, Clarice Carvalho
Coelho, Aline Thaynara de Moura
Dutra, Isabela Thamara Sabino
Teixeira, Sueleny Silva Ferreira
Ribeiro, Rosy Iara Maciel de Azambuja
Mourão, Marina de Moraes
Pereira, Rosiane Aparecida da Silva
Fonseca, Cristina Toscano
author_role author
author2 Araújo, Juliano Michel de
Carvalho, Gardênia Braz
Alves, Clarice Carvalho
Coelho, Aline Thaynara de Moura
Dutra, Isabela Thamara Sabino
Teixeira, Sueleny Silva Ferreira
Ribeiro, Rosy Iara Maciel de Azambuja
Mourão, Marina de Moraes
Pereira, Rosiane Aparecida da Silva
Fonseca, Cristina Toscano
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bernardes, Wilma Patrícia de Oliveira Santos
Araújo, Juliano Michel de
Carvalho, Gardênia Braz
Alves, Clarice Carvalho
Coelho, Aline Thaynara de Moura
Dutra, Isabela Thamara Sabino
Teixeira, Sueleny Silva Ferreira
Ribeiro, Rosy Iara Maciel de Azambuja
Mourão, Marina de Moraes
Pereira, Rosiane Aparecida da Silva
Fonseca, Cristina Toscano
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Vaccine
topic Schistosoma mansoni
Vaccine
description Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infeciosas e Parasitárias. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-17T13:17:58Z
dc.date.available.fl_str_mv 2020-02-17T13:17:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BERNARDES, Wilma Patrícia de Oliveira Santos et al. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research, v. 2019, p. 1-16, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40002
dc.identifier.issn.pt_BR.fl_str_mv 2314-8861
dc.identifier.doi.none.fl_str_mv 10.1155/2019/6793596
identifier_str_mv BERNARDES, Wilma Patrícia de Oliveira Santos et al. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research, v. 2019, p. 1-16, 2019.
2314-8861
10.1155/2019/6793596
url https://www.arca.fiocruz.br/handle/icict/40002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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