Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry

Detalhes bibliográficos
Autor(a) principal: Wang, Li-Ming
Data de Publicação: 2010
Outros Autores: Becker, J Sabine, Wu, Qi, Oliveira, Marcus F., Bozza, Fernando A., Schwager, Andrea L., Hoffman, John M., Morton, Kathryn A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37744
Resumo: 2020-12-10
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spelling Wang, Li-MingBecker, J SabineWu, QiOliveira, Marcus F.Bozza, Fernando A.Schwager, Andrea L.Hoffman, John M.Morton, Kathryn A.2019-12-10T13:28:55Z2019-12-10T13:28:55Z2010WANG, Li-Ming et al. Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry. Metallomics, v. 2, 348-353, 2010.1756-5901https://www.arca.fiocruz.br/handle/icict/3774410.1039/c003875j1756-591XengRoyal Society of ChemistryBioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-12-10University of Utah. Department of Radiology. Salt Lake City, UT, USA.Research Centre Juelich. Central Division of Analytical Chemistry. Juelich, Germany.University of Utah. Department of Radiology. Salt Lake City, UT, USA.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Medica. Laboratorio de Bioquimica Redox. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Biologia Estrutural e Bioimagem. Laboratório Associado de Inflamação e Metabolismo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clinica Evandro Chagas. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Biologia Estrutural e Bioimagem. Laboratório Associado de Inflamação e Metabolismo. Rio de Janeiro, RJ, Brasil.University of Utah. Graduate Program in Neuroscience. Salt Lake City, UT, USA.University of Utah. Department of Radiology. Salt Lake City, UT, USA / University of Utah. Department of Neurology. Salt Lake City, UT, USA.University of Utah. Department of Radiology. Salt Lake City, UT, USA.Copper may play an important role in the brain in aging and neurodegenerative diseases. We compare the active Cu uptake, Cu-containing enzyme levels, and total Cu distribution in the brains of young and aging mice. 67Cu was administered intravenously to 2, 7-9, and 14 month old mice. Active uptake of 67Cu in the brain was measured at 24 h by digital phosphor autoradiography. Cerebral superoxide dismutase-1 (SOD-1) and cytochrome-C oxidase subunit-1 (CCO-1) levels were analyzed by immunohistochemistry. The total Cu distribution in brain section was determined by imaging laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). In aging mice, active 67Cu uptake and SOD-1 levels were significantly decreased in the brain, whereas blood 67Cu and CCO-1 levels were similar for all mice, irrespective of age. Paradoxically, global Cu cerebral content was increased in aged mice, suggesting that regulation of active Cu uptake by the brain may be linked to total Cu levels in an attempt to maintain Cu homeostasis. However, focal areas of both decreased Cu uptake and Cu content were noted in the striatum and ventral cortex in aging mice. These focal areas of Cu deficit correspond to the regions of greatest reduction in SOD-1 in the aged mice. In aging, dysregulated Cu homeostasis may result in decreased SOD-1 levels, which may contribute to oxidative vulnerability of the aging brain. This study illustrates the importance of a multi-modality approach in studying the biodistribution and homeostasis of Cu in the brain.Aging brainCopperinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/37744/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Wang_Li-Ming_etal_INI_2010.pdfve_Wang_Li-Ming_etal_INI_2010.pdfapplication/pdf1498588https://www.arca.fiocruz.br/bitstream/icict/37744/2/ve_Wang_Li-Ming_etal_INI_2010.pdf230eb6ca0536ff1dee4e4db35bc8817eMD52TEXTve_Wang_Li-Ming_etal_INI_2010.pdf.txtve_Wang_Li-Ming_etal_INI_2010.pdf.txtExtracted texttext/plain26932https://www.arca.fiocruz.br/bitstream/icict/37744/3/ve_Wang_Li-Ming_etal_INI_2010.pdf.txt8fab3017ebc22313345296ee6c865ce7MD53icict/377442021-03-24 16:32:40.551oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
title Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
spellingShingle Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
Wang, Li-Ming
Aging brain
Copper
title_short Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
title_full Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
title_fullStr Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
title_full_unstemmed Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
title_sort Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry
author Wang, Li-Ming
author_facet Wang, Li-Ming
Becker, J Sabine
Wu, Qi
Oliveira, Marcus F.
Bozza, Fernando A.
Schwager, Andrea L.
Hoffman, John M.
Morton, Kathryn A.
author_role author
author2 Becker, J Sabine
Wu, Qi
Oliveira, Marcus F.
Bozza, Fernando A.
Schwager, Andrea L.
Hoffman, John M.
Morton, Kathryn A.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wang, Li-Ming
Becker, J Sabine
Wu, Qi
Oliveira, Marcus F.
Bozza, Fernando A.
Schwager, Andrea L.
Hoffman, John M.
Morton, Kathryn A.
dc.subject.en.pt_BR.fl_str_mv Aging brain
Copper
topic Aging brain
Copper
description 2020-12-10
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-12-10T13:28:55Z
dc.date.available.fl_str_mv 2019-12-10T13:28:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WANG, Li-Ming et al. Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry. Metallomics, v. 2, 348-353, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37744
dc.identifier.issn.pt_BR.fl_str_mv 1756-5901
dc.identifier.doi.none.fl_str_mv 10.1039/c003875j
dc.identifier.eissn.none.fl_str_mv 1756-591X
identifier_str_mv WANG, Li-Ming et al. Bioimaging of copper alterations in the aging mouse brain by autoradiography, laser ablation inductively coupled plasma mass spectrometry and immunohistochemistry. Metallomics, v. 2, 348-353, 2010.
1756-5901
10.1039/c003875j
1756-591X
url https://www.arca.fiocruz.br/handle/icict/37744
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37744/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/37744/2/ve_Wang_Li-Ming_etal_INI_2010.pdf
https://www.arca.fiocruz.br/bitstream/icict/37744/3/ve_Wang_Li-Ming_etal_INI_2010.pdf.txt
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