Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47859 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. |
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Carramaschi, Isabel N.Ferreira, Vítor dos S. B.Chagas, Thiago P.G.Corrêa, Lais L.Picão, Renata C,Queiroz, Margareth M. de C.Rangel, KaryneJardim, RodrigoMota, Fabio F. daZahner, Viviane2021-06-24T18:33:20Z2021-06-24T18:33:20Z2021CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021.2213-7165https://www.arca.fiocruz.br/handle/icict/4785910.1016/j.jgar.2020.11.021engScience DirectKlebsiella quasipneumoniaeMoscasSaúde PúblicaResistência a antibióticosKlebsiella quasipneumoniaeFliesPublic healthAntibiotic resistanceMultidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Objectives: Flies have been implicated in the dispersal of medically important bacteria including members of the genus Klebsiella between different environmental compartments. The aim of this study was to retrieve and characterize antibiotic-resistant bacteria from flies collected near to hospitals. Methods: Flies were collected in the vicinity of medical facilities and examined for bacteria demonstrating phenotypic resistance to ceftriaxone, followed by determination of phenotypic and genotypic resistance profiles. In addition, whole genome sequencing followed by phylogenetic analysis and resistance genotyping were performed with the multidrug-resistant (MDR) strain Lemef23, identified as Klebsiella quasipneumoniae subsp. similipneumoniae. Results: The strain Lemef23, classified by multiple locus sequence typing as novel ST 3397, harboured numerous resistance genes. The blaNDM was located on a Tn3000 element, a common genetic platform for the carriage of this gene in Brazil. Inference of phylogenetic orthology of strain Lemef23 and other clinical isolates suggested an anthropogenic origin. Conclusions: The findings of this study support the role of flies as vectors of MDR bacteria of clinical importance and provide the first record of blaNDM-1 and blaCTXM-15 in a Brazilian isolate of K. quasipneumoniae subsp. similipneumoniae, demonstrating the value of surveying insects as reservoirs of antibiotic resistance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47859/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALVivianeZaher_etal_IOC_2021.pdfVivianeZaher_etal_IOC_2021.pdfapplication/pdf755465https://www.arca.fiocruz.br/bitstream/icict/47859/2/VivianeZaher_etal_IOC_2021.pdf3e1f0912ea49f6fd00f1df8f5305a586MD52TEXTVivianeZaher_etal_IOC_2021.pdf.txtVivianeZaher_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| title |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| spellingShingle |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil Carramaschi, Isabel N. Klebsiella quasipneumoniae Moscas Saúde Pública Resistência a antibióticos Klebsiella quasipneumoniae Flies Public health Antibiotic resistance |
| title_short |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| title_full |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| title_fullStr |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| title_full_unstemmed |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| title_sort |
Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil |
| author |
Carramaschi, Isabel N. |
| author_facet |
Carramaschi, Isabel N. Ferreira, Vítor dos S. B. Chagas, Thiago P.G. Corrêa, Lais L. Picão, Renata C, Queiroz, Margareth M. de C. Rangel, Karyne Jardim, Rodrigo Mota, Fabio F. da Zahner, Viviane |
| author_role |
author |
| author2 |
Ferreira, Vítor dos S. B. Chagas, Thiago P.G. Corrêa, Lais L. Picão, Renata C, Queiroz, Margareth M. de C. Rangel, Karyne Jardim, Rodrigo Mota, Fabio F. da Zahner, Viviane |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carramaschi, Isabel N. Ferreira, Vítor dos S. B. Chagas, Thiago P.G. Corrêa, Lais L. Picão, Renata C, Queiroz, Margareth M. de C. Rangel, Karyne Jardim, Rodrigo Mota, Fabio F. da Zahner, Viviane |
| dc.subject.other.pt_BR.fl_str_mv |
Klebsiella quasipneumoniae Moscas Saúde Pública Resistência a antibióticos |
| topic |
Klebsiella quasipneumoniae Moscas Saúde Pública Resistência a antibióticos Klebsiella quasipneumoniae Flies Public health Antibiotic resistance |
| dc.subject.en.pt_BR.fl_str_mv |
Klebsiella quasipneumoniae Flies Public health Antibiotic resistance |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-06-24T18:33:20Z |
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2021-06-24T18:33:20Z |
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2021 |
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info:eu-repo/semantics/article |
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CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021. |
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https://www.arca.fiocruz.br/handle/icict/47859 |
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2213-7165 |
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10.1016/j.jgar.2020.11.021 |
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CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021. 2213-7165 10.1016/j.jgar.2020.11.021 |
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Science Direct |
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Science Direct |
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