Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15180 |
Resumo: | Universidade Federal da Bahia. Salvador, BA, Brasil |
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Sales, Caroline Brandi SchlaepferBuim, Marcilei Eliza CavichiolliSouza, Renata Oliveira deValverde, Ludmila de FaroMathias Machado, Maria CecíliaReis, Mitermayer Galvão dosSoares, Fernando AugustoRamos, Eduardo Antônio GonçalvesGurgel Rocha, Clarissa Araújo2016-08-02T17:26:09Z2016-08-02T17:26:09Z2016SALES, C. B. S. et al. Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study. Journal of Oral Pathology Medicine, feb. 2016.0904-2512https://www.arca.fiocruz.br/handle/icict/1518010.1111/jop.12398engWileyElevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Salvador, BA, BrasilAC Camargo Cancer Center. São Paulo, SP, BrasilUniversidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilAC Camargo Cancer Center. São Paulo, SP, BrasilUniversidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilNew blood vessel formation events are essential for tumor clonal expansion and progression. Despite the importance of vascular endothelial growth factor A (VEGFA) for tumor angiogenesis, few studies have evaluated the expression profile of this gene in oral squamous cell carcinoma (OSCC) and tumor margins (TM). This study aimed to evaluate the expression of the VEGFA gene and its protein in OSCC and TM. METHODS: Gene expression was evaluated in cryopreserved samples of OSCCs (n = 44), TM (n = 7), and normal mucosa from healthy patients (n = 3; NM). Quantitative PCRs were performed using the TaqMan system for the VEGFA gene, and gene expression was determined using the 2 ΔΔCQ method. For immunohistochemical evaluation, 27 OSCC samples were embedded in a tissue microarray (TMA) block and reactions were performed using the Advance system. RESULTS: VEGFA transcript levels were 1.7-fold higher in OSCC than in NM samples. VEGFA mRNA was overexpressed in TM samples compared to NM (3.4-fold) and OSCC (2.0-fold) samples. VEGFA transcript level was overexpressed in T3–T4 tumors, metastatic lymph nodes, and stage III-IV OSCCs. Immunoreactivity of the VEGFA protein was detected in the cytoplasm of parenchymal and stromal cells, mainly in endothelial cells and fibroblasts. CONCLUSION: Our results show that VEGFA was overexpressed in aggressive OSCCs and that VEGFA expression may be an important prognostic factor in this type of cancer. Finally, tumor margins may be involved in the production of angiogenic molecules.ImmunohistochemistryOral cancerReal-time polymerase chain reactionVascular endothelial growth factorinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15180/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSales_CBS Elevated VEGFA....pdfSales_CBS Elevated VEGFA....pdfapplication/pdf370427https://www.arca.fiocruz.br/bitstream/icict/15180/2/Sales_CBS%20%20Elevated%20VEGFA....pdf0b65b6518cec946d03d3ef0e378ddcceMD52TEXTSales_CBS Elevated VEGFA....pdf.txtSales_CBS Elevated VEGFA....pdf.txtExtracted texttext/plain21861https://www.arca.fiocruz.br/bitstream/icict/15180/3/Sales_CBS%20%20Elevated%20VEGFA....pdf.txt30e0dbc7753ec91c11ebbc82e23ebbe9MD53icict/151802023-03-15 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| dc.title.pt_BR.fl_str_mv |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| title |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| spellingShingle |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study Sales, Caroline Brandi Schlaepfer Immunohistochemistry Oral cancer Real-time polymerase chain reaction Vascular endothelial growth factor |
| title_short |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| title_full |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| title_fullStr |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| title_full_unstemmed |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| title_sort |
Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study |
| author |
Sales, Caroline Brandi Schlaepfer |
| author_facet |
Sales, Caroline Brandi Schlaepfer Buim, Marcilei Eliza Cavichiolli Souza, Renata Oliveira de Valverde, Ludmila de Faro Mathias Machado, Maria Cecília Reis, Mitermayer Galvão dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves Gurgel Rocha, Clarissa Araújo |
| author_role |
author |
| author2 |
Buim, Marcilei Eliza Cavichiolli Souza, Renata Oliveira de Valverde, Ludmila de Faro Mathias Machado, Maria Cecília Reis, Mitermayer Galvão dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves Gurgel Rocha, Clarissa Araújo |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sales, Caroline Brandi Schlaepfer Buim, Marcilei Eliza Cavichiolli Souza, Renata Oliveira de Valverde, Ludmila de Faro Mathias Machado, Maria Cecília Reis, Mitermayer Galvão dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves Gurgel Rocha, Clarissa Araújo |
| dc.subject.en.pt_BR.fl_str_mv |
Immunohistochemistry Oral cancer Real-time polymerase chain reaction Vascular endothelial growth factor |
| topic |
Immunohistochemistry Oral cancer Real-time polymerase chain reaction Vascular endothelial growth factor |
| description |
Universidade Federal da Bahia. Salvador, BA, Brasil |
| publishDate |
2016 |
| dc.date.accessioned.fl_str_mv |
2016-08-02T17:26:09Z |
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2016-08-02T17:26:09Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SALES, C. B. S. et al. Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study. Journal of Oral Pathology Medicine, feb. 2016. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/15180 |
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0904-2512 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/jop.12398 |
| identifier_str_mv |
SALES, C. B. S. et al. Elevated VEGFA mRNA levels in oral squamous cell carcinomas and tumor margins: a preliminary study. Journal of Oral Pathology Medicine, feb. 2016. 0904-2512 10.1111/jop.12398 |
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Wiley |
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Wiley |
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