Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.actatropica.2022.106579 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/60723 |
Resumo: | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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Amor, Ana Lúcia MorenoSantos, Leonardo NascimentoSilva, Eduardo SantosSantana, Marina Borges Rabêlo deBelitardo, Emília Maria Medeiros de AndradeSena, Flávia de AraújoCarvalho, Lain Pontes deFigueiredo, Camila A.Neves, Neuza Maria Alcântara2023-10-06T13:50:35Z2023-10-06T13:50:35Z2022AMOR, Ana Lúcia Moreno et al. Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro. Acta Tropica n. 234, p. 1-12, 2022.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/6072310.1016/j.actatropica.2022.106579Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Universidade Federal do Recôncavo da Bahia. Centro de Ciências da Saúde. Laboratório de Parasitologia. Santo Antônio de Jesus. BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Helminths possibly down-modulate immune responses to airborne allergens through the induction of a regulatory network. The identification of helminths bioactive molecules is highly desirable, given their immunomodulatory potential which could be used in immunotherapies for allergy and autoimmune diseases. To investigate the immunoregulatory potential of the adult Toxocara canis crude extract and ten protein fractions of its extract, human peripheral blood mononuclear cells (PBMC) from 10 allergic and 9 non-allergic individuals were cultivated, in vitro, in the presence or absence of these antigens, and their supernatants were evaluated for cytokine production (TGF-β, IL-10, IL-12, TNF-α, IL-6, IL-5, IL13, and IL-17). To determine the cell viability, the PBMC were cultivated for 24 h in the presence of the antigens and, following, they were subjected to a cytotoxicity assay. The viability of the PBMC was not affected by incubation with the T. canis antigens. As some fractions stimulated the production of immunoregulatory (TGF-β and/or IL-10), IL-12 and Th1 (TNF-α) cytokines, without stimulating Th2 cytokines (IL-5 and IL13) and IL-17, it was proposed that they would be potential candidates for further studies, especially involving the purification and characterization of specific proteins, which could be tested separately to evaluate their specific role as adjuvants in immunotherapy for inflammatory diseases.engElsevierMoléculas bioativasHelmintosImunomodulaçãoAlergiaBioactive moleculesHelminthImmunomodulationAllergyHelmintosImunomodulaçãoAlergia e ImunologiaToxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60723/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAmor, Ana Lúcia M. - Toxocara canis extract fractions promote mainly the production.pdfAmor, Ana Lúcia M. - Toxocara canis extract fractions promote mainly the production.pdfapplication/pdf7913041https://www.arca.fiocruz.br/bitstream/icict/60723/2/Amor%2c%20Ana%20L%c3%bacia%20M.%20-%20Toxocara%20canis%20extract%20fractions%20promote%20mainly%20the%20production.pdf3efa8bebaf6f67b4cfda8c46c9975edbMD52icict/607232023-10-06 10:50:36.521oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-06T13:50:36Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.en_US.fl_str_mv |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| title |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| spellingShingle |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro Amor, Ana Lúcia Moreno Moléculas bioativas Helmintos Imunomodulação Alergia Bioactive molecules Helminth Immunomodulation Allergy Helmintos Imunomodulação Alergia e Imunologia |
| title_short |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| title_full |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| title_fullStr |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| title_full_unstemmed |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| title_sort |
Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro |
| author |
Amor, Ana Lúcia Moreno |
| author_facet |
Amor, Ana Lúcia Moreno Santos, Leonardo Nascimento Silva, Eduardo Santos Santana, Marina Borges Rabêlo de Belitardo, Emília Maria Medeiros de Andrade Sena, Flávia de Araújo Carvalho, Lain Pontes de Figueiredo, Camila A. Neves, Neuza Maria Alcântara |
| author_role |
author |
| author2 |
Santos, Leonardo Nascimento Silva, Eduardo Santos Santana, Marina Borges Rabêlo de Belitardo, Emília Maria Medeiros de Andrade Sena, Flávia de Araújo Carvalho, Lain Pontes de Figueiredo, Camila A. Neves, Neuza Maria Alcântara |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amor, Ana Lúcia Moreno Santos, Leonardo Nascimento Silva, Eduardo Santos Santana, Marina Borges Rabêlo de Belitardo, Emília Maria Medeiros de Andrade Sena, Flávia de Araújo Carvalho, Lain Pontes de Figueiredo, Camila A. Neves, Neuza Maria Alcântara |
| dc.subject.other.en_US.fl_str_mv |
Moléculas bioativas Helmintos Imunomodulação Alergia |
| topic |
Moléculas bioativas Helmintos Imunomodulação Alergia Bioactive molecules Helminth Immunomodulation Allergy Helmintos Imunomodulação Alergia e Imunologia |
| dc.subject.en.en_US.fl_str_mv |
Bioactive molecules Helminth Immunomodulation Allergy |
| dc.subject.decs.en_US.fl_str_mv |
Helmintos Imunomodulação Alergia e Imunologia |
| description |
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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2022 |
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2022 |
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2023-10-06T13:50:35Z |
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2023-10-06T13:50:35Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
AMOR, Ana Lúcia Moreno et al. Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro. Acta Tropica n. 234, p. 1-12, 2022. |
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https://www.arca.fiocruz.br/handle/icict/60723 |
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0001-706X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2022.106579 |
| identifier_str_mv |
AMOR, Ana Lúcia Moreno et al. Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro. Acta Tropica n. 234, p. 1-12, 2022. 0001-706X 10.1016/j.actatropica.2022.106579 |
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Elsevier |
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Elsevier |
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