Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse

Detalhes bibliográficos
Autor(a) principal: Esquenazi, Danuza
Data de Publicação: 2015
Outros Autores: Alvim, Iris Maria Peixoto, Pinheiro, Roberta Olmo, Oliveira, Eliane Barbosa de, Moreira, Lilian de Oliveira, Sarno, Euzenir Nunes, Nery, José Augusto da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11511
Resumo: Correction: Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse Danuza Esquenazi, Iris Maria Peixoto Alvim, Roberta Olmo Pinheiro, Eliane Barbosa de Oliveira,Lilian de Oliveira Moreira, Euzenir Nunes Sarno,Jose Augusto da Costa Nery. Notice of Republication This article was republishedon Jul 23,2015, to correctim properly-formatted Greek letters throughout the text. Please download this article again to view the correct version. Reference 1. Esquenazi D,AlvimI M P, Pinheiro R O, Oliveira E Bd, Moreira Ld O,Sarno E N,et al. (2015) Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical presentation of Multibacillary Leprosy Relapse. PLoS ONE doi:10.1371/journal. pone.0127416 PMID:25992795.
id CRUZ_d9657a0c12b8f97bbe84aae2dfe807bf
oai_identifier_str oai:www.arca.fiocruz.br:icict/11511
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Esquenazi, DanuzaAlvim, Iris Maria PeixotoPinheiro, Roberta OlmoOliveira, Eliane Barbosa deMoreira, Lilian de OliveiraSarno, Euzenir NunesNery, José Augusto da Costa2015-08-19T13:49:25Z2015-08-19T13:49:25Z2015ESQUENAZI, Danuza; et al. Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse. Plos One, v. 10, n. 5, p. 1-17, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1151110.1371/journal.pone.0127416Correction: Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse Danuza Esquenazi, Iris Maria Peixoto Alvim, Roberta Olmo Pinheiro, Eliane Barbosa de Oliveira,Lilian de Oliveira Moreira, Euzenir Nunes Sarno,Jose Augusto da Costa Nery. Notice of Republication This article was republishedon Jul 23,2015, to correctim properly-formatted Greek letters throughout the text. Please download this article again to view the correct version. Reference 1. Esquenazi D,AlvimI M P, Pinheiro R O, Oliveira E Bd, Moreira Ld O,Sarno E N,et al. (2015) Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical presentation of Multibacillary Leprosy Relapse. PLoS ONE doi:10.1371/journal. pone.0127416 PMID:25992795.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Centro de Ciências Médicas. Departamento de Patologia e Laboratórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Background: Despite the efficacy of multidrug therapy, surviving Mycobacterium leprae causes relapse in some leprosy patients, and these patients present signs and symptoms of disease after healing. This study focused on the cellular immune response in relapsed multibacillary patients but also included non-relapsed multibacillary cured individuals, newly diagnosed and untreated multibacillary patients, paucibacillary patients just before the beginning of treatment, and voluntary healthy individuals for comparative analysis.Methodology/Principal Findings Inhibition of CD86 expression in the blood-derived monocytes and dendritic cells of relapsed multibacillary patients, either ex vivo or after M. leprae antigen stimulation was observed by flow cytometry. In addition, no significant changes in Interferon-gamma (IFN-g) expression were observed in 5-day culture supernatants of relapsed patients in response to M. leprae, neither before nor after treatment, as measured by ELISA. However, these patients demonstrated a significant increase in central memory CD4+ and CD8+ M. lepraespecific T cells, as assessed by multiparametric flow cytometry. The increase in frequency of central memory T cells in relapsed patients strongly correlated with the bacillary index and the number of skin lesions observed in these subjects. Moreover, cytokine multiplex analysis demonstrated significant antigen-specific production of Interlukin-1beta (IL-1b), IL-6, and Tumour Necrosis Factor (TNF) in the relapsed group with extremely low IL-10 production, which resulted in a high TNF/IL-10 ratio.Conclusions/Significance Inhibition of CD86 expression may function to reduce effector T cell responses against the M. leprae antigen. Furthermore, the predominance of central memory T cells in association with the high TNF/IL-10 ratio and no observed IFN-g production may be related to the pathogenesis of relapse in multibacillary leprosy. Therefore, our findings may be a direct result of the clinical presentation, including a number of skin lesions and bacterial load, of relapsed patients. To our knowledge, this is the first study correlating immune response parameters with the clinical presentation of relapsed multibacillary patients.engPlos OneCorrelation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMultibacillary LeprosyCentral Memory T Cell ExpressionProinflammatory Cytokine ProductionHanseníase MultibacilarHanseníase MultibacilarCitocinaMycobacterium lepraeResistência a Múltiplos Medicamentosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11511/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALdanuza_esquenazi_etal_IOC_2015.pdfapplication/pdf747651https://www.arca.fiocruz.br/bitstream/icict/11511/2/danuza_esquenazi_etal_IOC_2015.pdf579057cff06bca7d5761944b7fe781e9MD52Correction-handle_11511_IOC_2015.pdfCorrection-handle_11511_IOC_2015.pdfapplication/pdf113676https://www.arca.fiocruz.br/bitstream/icict/11511/4/Correction-handle_11511_IOC_2015.pdf15318768247c95d3b994eba5aa7cd22dMD54TEXTdanuza_esquenazi_etal_IOC_2015.pdf.txtdanuza_esquenazi_etal_IOC_2015.pdf.txtExtracted texttext/plain61743https://www.arca.fiocruz.br/bitstream/icict/11511/3/danuza_esquenazi_etal_IOC_2015.pdf.txte27706636579aedd80f7f27f0135d14fMD53Correction-handle_11511_IOC_2015.pdf.txtCorrection-handle_11511_IOC_2015.pdf.txtExtracted texttext/plain1582https://www.arca.fiocruz.br/bitstream/icict/11511/5/Correction-handle_11511_IOC_2015.pdf.txt7d2fdb9d6ff88c2e4eb1789b1c66ea10MD55icict/115112022-06-24 13:02:57.922oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:02:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
title Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
spellingShingle Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
Esquenazi, Danuza
Multibacillary Leprosy
Central Memory T Cell Expression
Proinflammatory Cytokine Production
Hanseníase Multibacilar
Hanseníase Multibacilar
Citocina
Mycobacterium leprae
Resistência a Múltiplos Medicamentos
title_short Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
title_full Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
title_fullStr Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
title_full_unstemmed Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
title_sort Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse
author Esquenazi, Danuza
author_facet Esquenazi, Danuza
Alvim, Iris Maria Peixoto
Pinheiro, Roberta Olmo
Oliveira, Eliane Barbosa de
Moreira, Lilian de Oliveira
Sarno, Euzenir Nunes
Nery, José Augusto da Costa
author_role author
author2 Alvim, Iris Maria Peixoto
Pinheiro, Roberta Olmo
Oliveira, Eliane Barbosa de
Moreira, Lilian de Oliveira
Sarno, Euzenir Nunes
Nery, José Augusto da Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Esquenazi, Danuza
Alvim, Iris Maria Peixoto
Pinheiro, Roberta Olmo
Oliveira, Eliane Barbosa de
Moreira, Lilian de Oliveira
Sarno, Euzenir Nunes
Nery, José Augusto da Costa
dc.subject.en.pt_BR.fl_str_mv Multibacillary Leprosy
Central Memory T Cell Expression
Proinflammatory Cytokine Production
Hanseníase Multibacilar
topic Multibacillary Leprosy
Central Memory T Cell Expression
Proinflammatory Cytokine Production
Hanseníase Multibacilar
Hanseníase Multibacilar
Citocina
Mycobacterium leprae
Resistência a Múltiplos Medicamentos
dc.subject.decs.pt_BR.fl_str_mv Hanseníase Multibacilar
Citocina
Mycobacterium leprae
Resistência a Múltiplos Medicamentos
description Correction: Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse Danuza Esquenazi, Iris Maria Peixoto Alvim, Roberta Olmo Pinheiro, Eliane Barbosa de Oliveira,Lilian de Oliveira Moreira, Euzenir Nunes Sarno,Jose Augusto da Costa Nery. Notice of Republication This article was republishedon Jul 23,2015, to correctim properly-formatted Greek letters throughout the text. Please download this article again to view the correct version. Reference 1. Esquenazi D,AlvimI M P, Pinheiro R O, Oliveira E Bd, Moreira Ld O,Sarno E N,et al. (2015) Correlation between Central Memory TCell Expression and Proinflammatory Cytokine Production with Clinical presentation of Multibacillary Leprosy Relapse. PLoS ONE doi:10.1371/journal. pone.0127416 PMID:25992795.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:25Z
dc.date.available.fl_str_mv 2015-08-19T13:49:25Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ESQUENAZI, Danuza; et al. Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse. Plos One, v. 10, n. 5, p. 1-17, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11511
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0127416
identifier_str_mv ESQUENAZI, Danuza; et al. Correlation between Central Memory T Cell Expression and Proinflammatory Cytokine Production with Clinical Presentation of Multibacillary Leprosy Relapse. Plos One, v. 10, n. 5, p. 1-17, 2015.
1932-6203
10.1371/journal.pone.0127416
url https://www.arca.fiocruz.br/handle/icict/11511
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11511/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/11511/2/danuza_esquenazi_etal_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/11511/4/Correction-handle_11511_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/11511/3/danuza_esquenazi_etal_IOC_2015.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/11511/5/Correction-handle_11511_IOC_2015.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
579057cff06bca7d5761944b7fe781e9
15318768247c95d3b994eba5aa7cd22d
e27706636579aedd80f7f27f0135d14f
7d2fdb9d6ff88c2e4eb1789b1c66ea10
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008883359154176

Registros relacionados