Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

Detalhes bibliográficos
Autor(a) principal: Ferraz, T. P. L
Data de Publicação: 2004
Outros Autores: Fiúza, M. C, Santos, M. L. A. dos, Pontes-de-Carvalho, Lain Carlos, Soares, Neci Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3797
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
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spelling Ferraz, T. P. LFiúza, M. CSantos, M. L. A. dosPontes-de-Carvalho, Lain CarlosSoares, Neci Matos2012-03-14T19:18:16Z2012-03-14T19:18:16Z2004FERRAZ, T.P. et al. Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. Journal of Biochemical and Biophysics Methods, v. 58, n. 3, p. 187-193, mar. 2004.0165-022Xhttps://www.arca.fiocruz.br/handle/icict/3797doi:10.1016/j.jbbm.2003.10.008engComparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fractionLipid extractionProteinsSilicaOrganic solventsDetergentTécnicas de Laboratório ClínicoLipídeosSoroPolietilenoglicóisPreservação biológicaSensibilidade e EspecificidadeProteinasDióxido de SilícioSolventesHumanosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFerraz TP Comparison of six methods....pdfFerraz TP Comparison of six methods....pdfapplication/pdf206713https://www.arca.fiocruz.br/bitstream/icict/3797/1/Ferraz%20TP%20Comparison%20of%20six%20methods....pdfd6f3ae6ffe05064843dd31f40a194e93MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3797/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTFerraz TP Comparison of six methods....pdf.txtFerraz TP Comparison of six methods....pdf.txtExtracted texttext/plain15196https://www.arca.fiocruz.br/bitstream/icict/3797/5/Ferraz%20TP%20Comparison%20of%20six%20methods....pdf.txtfd57932eb9cf89ae0054acb4fa5f52f2MD55THUMBNAILFerraz TP Comparison of six methods....pdf.jpgFerraz TP Comparison of six methods....pdf.jpgGenerated Thumbnailimage/jpeg1985https://www.arca.fiocruz.br/bitstream/icict/3797/4/Ferraz%20TP%20Comparison%20of%20six%20methods....pdf.jpgb7703bcbf410bf28397546777f71f7b4MD54icict/37972023-03-15 14:33:00.87oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
spellingShingle Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
Ferraz, T. P. L
Lipid extraction
Proteins
Silica
Organic solvents
Detergent
Técnicas de Laboratório Clínico
Lipídeos
Soro
Polietilenoglicóis
Preservação biológica
Sensibilidade e Especificidade
Proteinas
Dióxido de Silício
Solventes
Humanos
title_short Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_full Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_fullStr Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_full_unstemmed Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
title_sort Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation
author Ferraz, T. P. L
author_facet Ferraz, T. P. L
Fiúza, M. C
Santos, M. L. A. dos
Pontes-de-Carvalho, Lain Carlos
Soares, Neci Matos
author_role author
author2 Fiúza, M. C
Santos, M. L. A. dos
Pontes-de-Carvalho, Lain Carlos
Soares, Neci Matos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferraz, T. P. L
Fiúza, M. C
Santos, M. L. A. dos
Pontes-de-Carvalho, Lain Carlos
Soares, Neci Matos
dc.subject.en.pt_BR.fl_str_mv Lipid extraction
Proteins
Silica
Organic solvents
Detergent
topic Lipid extraction
Proteins
Silica
Organic solvents
Detergent
Técnicas de Laboratório Clínico
Lipídeos
Soro
Polietilenoglicóis
Preservação biológica
Sensibilidade e Especificidade
Proteinas
Dióxido de Silício
Solventes
Humanos
dc.subject.decs.pt_BR.fl_str_mv Técnicas de Laboratório Clínico
Lipídeos
Soro
Polietilenoglicóis
Preservação biológica
Sensibilidade e Especificidade
Proteinas
Dióxido de Silício
Solventes
Humanos
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2012-03-14T19:18:16Z
dc.date.available.fl_str_mv 2012-03-14T19:18:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRAZ, T.P. et al. Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. Journal of Biochemical and Biophysics Methods, v. 58, n. 3, p. 187-193, mar. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3797
dc.identifier.issn.none.fl_str_mv 0165-022X
dc.identifier.doi.none.fl_str_mv doi:10.1016/j.jbbm.2003.10.008
identifier_str_mv FERRAZ, T.P. et al. Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. Journal of Biochemical and Biophysics Methods, v. 58, n. 3, p. 187-193, mar. 2004.
0165-022X
doi:10.1016/j.jbbm.2003.10.008
url https://www.arca.fiocruz.br/handle/icict/3797
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language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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