High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

Detalhes bibliográficos
Autor(a) principal: Vilela, Felipe Pinheiro
Data de Publicação: 2020
Outros Autores: Rodrigues, Dalia dos Prazeres, Costa, Renata Garcia, Casas, Monique Ribeiro Tibas, Falcão, Juliana Pfrimer, Campioni, Fábio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44890
Resumo: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
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spelling Vilela, Felipe PinheiroRodrigues, Dalia dos PrazeresCosta, Renata GarciaCasas, Monique Ribeiro TibasFalcão, Juliana PfrimerCampioni, Fábio2020-12-15T13:53:04Z2020-12-15T13:53:04Z2020VILELA, Felipe Pinheiro et al. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33 year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, p. 497-509, Jun. 2020.1517-8382https://www.arca.fiocruz.br/handle/icict/4489010.1007/s42770-019-00156-51678-4405engSociedade Brasileira de MicrobiologiaCRISPRCRISPR-MVLSTMLSTSalmonella DublinGenes de virulênciaCRISPRCRISPR-MVLSTMLSTSalmonella DublinVirulence genesHigh similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Instituto Adolfo Lutz. Centro de Bacteriologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.almonella Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease with high rates of mortality in cattle and occasionally infects humans. Despite the importance of this serovar, there is a lack of studies in Brazil. The aim of this study was to characterize the genetic diversity of 112 S. Dublin strains isolated from humans and animals in Brazil by CRISPR and CRISPR-MVLST and the relatedness among strains by MLST. In addition, the frequency of some important virulence genes was verified. The strains studied belonged to nine different sequence types, being all of them single- or double-locus variants of the ST10. CRISPR discriminated the strains into 69 subtypes with a similarity ≥ 84.4% and CRISPR-MVLST into 72 subtypes with a similarity ≥ 84.7%. The virulence genes ratB, lpfA, mgtC, avrA, sopB, sopE2, sifA, sseA, ssrA, csgA, fliC, and sinH were found in all the strains studied, while spvB, spvC, sodCl, rpoS, sipA, sipD, invA, and hilA were detected in ≥ 93.7% of the strains. In conclusion, the high similarity among the strains reinforces the clonal nature of the strains of this serovar that may have descended from a common ancestor that little differed over 33 years in Brazil. CRISPR and CRISPR-MVLST showed to be good alternatives to type S. Dublin strains. MLST suggested that S. Dublin strains from Brazil were phylogenetically related to strains from other parts of the globe. Moreover, the high frequency of virulence genes among the strains studied reinforces the capacity of S. Dublin to cause invasive diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44890/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDaliaPrazeres_Rodrigues_etal_IOC_2020.pdfDaliaPrazeres_Rodrigues_etal_IOC_2020.pdfapplication/pdf805578https://www.arca.fiocruz.br/bitstream/icict/44890/2/DaliaPrazeres_Rodrigues_etal_IOC_2020.pdf05c3002686e362fce27c4eb36f607cfdMD52TEXTDaliaPrazeres_Rodrigues_etal_IOC_2020.pdf.txtDaliaPrazeres_Rodrigues_etal_IOC_2020.pdf.txtExtracted texttext/plain49122https://www.arca.fiocruz.br/bitstream/icict/44890/3/DaliaPrazeres_Rodrigues_etal_IOC_2020.pdf.txtdafa572b7cdec6628a2a005ad6ed3e1eMD53icict/448902020-12-16 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dc.title.pt_BR.fl_str_mv High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
title High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
spellingShingle High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
Vilela, Felipe Pinheiro
CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Genes de virulência
CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Virulence genes
title_short High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
title_full High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
title_fullStr High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
title_full_unstemmed High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
title_sort High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil
author Vilela, Felipe Pinheiro
author_facet Vilela, Felipe Pinheiro
Rodrigues, Dalia dos Prazeres
Costa, Renata Garcia
Casas, Monique Ribeiro Tibas
Falcão, Juliana Pfrimer
Campioni, Fábio
author_role author
author2 Rodrigues, Dalia dos Prazeres
Costa, Renata Garcia
Casas, Monique Ribeiro Tibas
Falcão, Juliana Pfrimer
Campioni, Fábio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vilela, Felipe Pinheiro
Rodrigues, Dalia dos Prazeres
Costa, Renata Garcia
Casas, Monique Ribeiro Tibas
Falcão, Juliana Pfrimer
Campioni, Fábio
dc.subject.other.pt_BR.fl_str_mv CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Genes de virulência
topic CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Genes de virulência
CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Virulence genes
dc.subject.en.pt_BR.fl_str_mv CRISPR
CRISPR-MVLST
MLST
Salmonella Dublin
Virulence genes
description Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-15T13:53:04Z
dc.date.available.fl_str_mv 2020-12-15T13:53:04Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VILELA, Felipe Pinheiro et al. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33 year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, p. 497-509, Jun. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44890
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.none.fl_str_mv 10.1007/s42770-019-00156-5
dc.identifier.eissn.none.fl_str_mv 1678-4405
identifier_str_mv VILELA, Felipe Pinheiro et al. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33 year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, p. 497-509, Jun. 2020.
1517-8382
10.1007/s42770-019-00156-5
1678-4405
url https://www.arca.fiocruz.br/handle/icict/44890
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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