Detrimental Effect of Ozone on Pathogenic Bacteria

Detalhes bibliográficos
Autor(a) principal: Rangel, Karyne
Data de Publicação: 2022
Outros Autores: Cabral, Fellipe O., Lechuga, Guilherme C., Carvalho, João P. R. S., Villas-Bôas, Maria H. S., Midlej, Victor, De-Simone, Salvatore G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51597
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciências e Tecnologia para Inovação em Doenças da População Negligenciadas; Rio de Janeiro, RJ, Brasil.
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spelling Rangel, KaryneCabral, Fellipe O.Lechuga, Guilherme C.Carvalho, João P. R. S.Villas-Bôas, Maria H. S.Midlej, VictorDe-Simone, Salvatore G.2022-03-07T17:57:16Z2022-03-07T17:57:16Z2022RANGEL, Karyne et al. Detrimental Effect of Ozone on Pathogenic Bacteria. Microorganisms, v. 10, n. 40. p. 1 - 17, 2022.2076-2607https://www.arca.fiocruz.br/handle/icict/5159710.3390/ microorganisms10010040engMDPIPseudomonas aeruginosaOzônioEfeito prejudicialBactéria patogênicaResistência antimicrobianaAcinetobacter baumanniiOxigênio intracelular reativoOzoneDetrimental effectPathogenic bacteriaAntimicrobial resistanceR.O.SAcinetobacter baumanniiPseudomonas aeruginosaDetrimental Effect of Ozone on Pathogenic Bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciências e Tecnologia para Inovação em Doenças da População Negligenciadas; Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciências e Tecnologia para Inovação em Doenças da População Negligenciadas; Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciências e Tecnologia para Inovação em Doenças da População Negligenciadas; Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. NIterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Background: Disinfection of medical devices designed for clinical use associated or not with the growing area of tissue engineering is an urgent need. However, traditional disinfection methods are not always suitable for some biomaterials, especially those sensitive to chemical, thermal, or radiation. Therefore, the objective of this study was to evaluate the minimal concentration of ozone gas (O3) necessary to control and kill a set of sensitive or multi-resistant Gram-positive and Gram-negative bacteria. The cell viability, membrane permeability, and the levels of reactive intracellular oxygen (ROS) species were also investigated; (2) Material and Methods: Four standard strains and a clinical MDR strain were exposed to low doses of ozone at different concentrations and times. Bacterial inactivation (cultivability, membrane damage) was investigated using colony counts, resazurin as a metabolic indicator, and propidium iodide (PI). A fluorescent probe (H2DCFDA) was used for the ROS analyses; (3) Results: No reduction in the count colony was detected after O3 exposure compared to the control group. However, the cell viability of E. coli (30%), P. aeruginosa (25%), and A. baumannii (15%) was reduced considerably. The bacterial membrane of all strains was not affected by O3 but presented a significant increase of ROS in E. coli (90 14%), P. aeruginosa (62.5 19%), and A. baumanni (52.6 5%); (4) Conclusion: Low doses of ozone were able to interfere in the cell viability of most strains studied, and although it does not cause damage to the bacterial membrane, increased levels of reactive ROS are responsible for causing a detrimental effect in the lipids, proteins, and DNA metabolism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51597/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALVitorMidlej_Salvatore Simone_etal_IOC_2022.pdfVitorMidlej_Salvatore Simone_etal_IOC_2022.pdfapplication/pdf3623241https://www.arca.fiocruz.br/bitstream/icict/51597/2/VitorMidlej_Salvatore%20Simone_etal_IOC_2022.pdf06a4ef4d4ac5bd91b89c88acceb3a741MD52icict/515972023-09-05 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dc.title.pt_BR.fl_str_mv Detrimental Effect of Ozone on Pathogenic Bacteria
title Detrimental Effect of Ozone on Pathogenic Bacteria
spellingShingle Detrimental Effect of Ozone on Pathogenic Bacteria
Rangel, Karyne
Pseudomonas aeruginosa
Ozônio
Efeito prejudicial
Bactéria patogênica
Resistência antimicrobiana
Acinetobacter baumannii
Oxigênio intracelular reativo
Ozone
Detrimental effect
Pathogenic bacteria
Antimicrobial resistance
R.O.S
Acinetobacter baumannii
Pseudomonas aeruginosa
title_short Detrimental Effect of Ozone on Pathogenic Bacteria
title_full Detrimental Effect of Ozone on Pathogenic Bacteria
title_fullStr Detrimental Effect of Ozone on Pathogenic Bacteria
title_full_unstemmed Detrimental Effect of Ozone on Pathogenic Bacteria
title_sort Detrimental Effect of Ozone on Pathogenic Bacteria
author Rangel, Karyne
author_facet Rangel, Karyne
Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
author_role author
author2 Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rangel, Karyne
Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
dc.subject.other.pt_BR.fl_str_mv Pseudomonas aeruginosa
Ozônio
Efeito prejudicial
Bactéria patogênica
Resistência antimicrobiana
Acinetobacter baumannii
Oxigênio intracelular reativo
topic Pseudomonas aeruginosa
Ozônio
Efeito prejudicial
Bactéria patogênica
Resistência antimicrobiana
Acinetobacter baumannii
Oxigênio intracelular reativo
Ozone
Detrimental effect
Pathogenic bacteria
Antimicrobial resistance
R.O.S
Acinetobacter baumannii
Pseudomonas aeruginosa
dc.subject.en.pt_BR.fl_str_mv Ozone
Detrimental effect
Pathogenic bacteria
Antimicrobial resistance
R.O.S
Acinetobacter baumannii
Pseudomonas aeruginosa
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciências e Tecnologia para Inovação em Doenças da População Negligenciadas; Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-07T17:57:16Z
dc.date.available.fl_str_mv 2022-03-07T17:57:16Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RANGEL, Karyne et al. Detrimental Effect of Ozone on Pathogenic Bacteria. Microorganisms, v. 10, n. 40. p. 1 - 17, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51597
dc.identifier.issn.pt_BR.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/ microorganisms10010040
identifier_str_mv RANGEL, Karyne et al. Detrimental Effect of Ozone on Pathogenic Bacteria. Microorganisms, v. 10, n. 40. p. 1 - 17, 2022.
2076-2607
10.3390/ microorganisms10010040
url https://www.arca.fiocruz.br/handle/icict/51597
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51597/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51597/2/VitorMidlej_Salvatore%20Simone_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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