PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/29349 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. |
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Díaz Acosta, Chyntia CarolinaDias, André AlvesRosa, Thabatta Leal Silveira AndrezoSilva, Leonardo Ribeiro BatistaRosa, Patricia SammarcoPinto, Thiago Gomes ToledoCosta, Fabrício da Mota RamalhoLara, Flávio AlvesRodrigues, Luciana SilvaMattos, Katherine AntunesSarno, Euzenir NunesBozza, Patrícia TorresGuilhot, ChristophePinho, Márcia de BerrêdoPessolani, Maria Cristina Vidal2018-10-04T16:24:25Z2018-10-04T16:24:25Z2018DIAZ ACOSTA, Chyntia Carolina; et al. PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves. PLoS Pathog., v.14, n.7, e1007151, 33p, July 2018.1553-7366https://www.arca.fiocruz.br/handle/icict/2934910.1371/journal.ppat.10071511553-7374engPublic Library of ScienceMycobacterium lepraeHanseníasenervos periféricosPGL Ibactérias vivasMycobacterium lepraeLeprosyperipheral nervesPGL I expressionlive bacteriaPGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nervesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Université de Toulouse. Institut de Pharmacologie et de Biologie Structurale. Toulouse, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Mycobacterium leprae, an obligate intracellular bacillus, infects Schwann cells (SCs), leading to peripheral nerve damage, the most severe leprosy symptom. In the present study, we revisited the involvement of phenolic glycolipid I (PGL I), an abundant, private, surface M. leprae molecule, in M. leprae-SC interaction by using a recombinant strain of M. bovis BCG engineered to express this glycolipid. We demonstrate that PGL I is essential for bacterial adhesion and SC internalization. We also show that live mycobacterium-producing PGL I induces the expression of the endocytic mannose receptor (MR/CD206) in infected cells in a peroxisome proliferator-activated receptor gamma (PPARγ)-dependent manner. Of note, blocking mannose recognition decreased bacterial entry and survival, pointing to a role for this alternative recognition pathway in bacterial pathogenesis in the nerve. Moreover, an active crosstalk between CD206 and the nuclear receptor PPARγ was detected that led to the induction of lipid droplets (LDs) formation and prostaglandin E2 (PGE2), previously described as fundamental players in bacterial pathogenesis. Finally, this pathway was shown to induce IL-8 secretion. Altogether, our study provides evidence that the entry of live M. leprae through PGL I recognition modulates the SC phenotype, favoring intracellular bacterial persistence with the concomitant secretion of inflammatory mediators that may ultimately be involved in neuroinflammation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29349/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcynthia_acosta_etal_IOC_2018.pdfcynthia_acosta_etal_IOC_2018.pdfapplication/pdf26727249https://www.arca.fiocruz.br/bitstream/icict/29349/2/cynthia_acosta_etal_IOC_2018.pdfd2965be67d60acdef7cee33b1fcdd459MD52TEXTcynthia_acosta_etal_IOC_2018.pdf.txtcynthia_acosta_etal_IOC_2018.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| title |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| spellingShingle |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves Díaz Acosta, Chyntia Carolina Mycobacterium leprae Hanseníase nervos periféricos PGL I bactérias vivas Mycobacterium leprae Leprosy peripheral nerves PGL I expression live bacteria |
| title_short |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| title_full |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| title_fullStr |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| title_full_unstemmed |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| title_sort |
PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves |
| author |
Díaz Acosta, Chyntia Carolina |
| author_facet |
Díaz Acosta, Chyntia Carolina Dias, André Alves Rosa, Thabatta Leal Silveira Andrezo Silva, Leonardo Ribeiro Batista Rosa, Patricia Sammarco Pinto, Thiago Gomes Toledo Costa, Fabrício da Mota Ramalho Lara, Flávio Alves Rodrigues, Luciana Silva Mattos, Katherine Antunes Sarno, Euzenir Nunes Bozza, Patrícia Torres Guilhot, Christophe Pinho, Márcia de Berrêdo Pessolani, Maria Cristina Vidal |
| author_role |
author |
| author2 |
Dias, André Alves Rosa, Thabatta Leal Silveira Andrezo Silva, Leonardo Ribeiro Batista Rosa, Patricia Sammarco Pinto, Thiago Gomes Toledo Costa, Fabrício da Mota Ramalho Lara, Flávio Alves Rodrigues, Luciana Silva Mattos, Katherine Antunes Sarno, Euzenir Nunes Bozza, Patrícia Torres Guilhot, Christophe Pinho, Márcia de Berrêdo Pessolani, Maria Cristina Vidal |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Díaz Acosta, Chyntia Carolina Dias, André Alves Rosa, Thabatta Leal Silveira Andrezo Silva, Leonardo Ribeiro Batista Rosa, Patricia Sammarco Pinto, Thiago Gomes Toledo Costa, Fabrício da Mota Ramalho Lara, Flávio Alves Rodrigues, Luciana Silva Mattos, Katherine Antunes Sarno, Euzenir Nunes Bozza, Patrícia Torres Guilhot, Christophe Pinho, Márcia de Berrêdo Pessolani, Maria Cristina Vidal |
| dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium leprae Hanseníase nervos periféricos PGL I bactérias vivas |
| topic |
Mycobacterium leprae Hanseníase nervos periféricos PGL I bactérias vivas Mycobacterium leprae Leprosy peripheral nerves PGL I expression live bacteria |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium leprae Leprosy peripheral nerves PGL I expression live bacteria |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. |
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2018 |
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2018-10-04T16:24:25Z |
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2018-10-04T16:24:25Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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DIAZ ACOSTA, Chyntia Carolina; et al. PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves. PLoS Pathog., v.14, n.7, e1007151, 33p, July 2018. |
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https://www.arca.fiocruz.br/handle/icict/29349 |
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1553-7366 |
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10.1371/journal.ppat.1007151 |
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1553-7374 |
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DIAZ ACOSTA, Chyntia Carolina; et al. PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves. PLoS Pathog., v.14, n.7, e1007151, 33p, July 2018. 1553-7366 10.1371/journal.ppat.1007151 1553-7374 |
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