Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains

Detalhes bibliográficos
Autor(a) principal: Requião, Rodrigo D.
Data de Publicação: 2020
Outros Autores: Carneiro, Rodolfo L., Moreira, Mariana Hoyer, Ribeiro-Alves, Marcelo, Rossetto, Silvana, Palhano, Fernando L., Domitrovic, Tatiana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1038/s41598-020-62328-w
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40739
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
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spelling Requião, Rodrigo D.Carneiro, Rodolfo L.Moreira, Mariana HoyerRibeiro-Alves, MarceloRossetto, SilvanaPalhano, Fernando L.Domitrovic, Tatiana2020-04-13T22:55:37Z2020-04-13T22:55:37Z2020REQUIÃO, Rodrigo D. et al. Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains. Scientific Reports, v. 10, n. 1, p. 1-12, 2020.2045-2322https://www.arca.fiocruz.br/handle/icict/4073910.1038/s41598-020-62328-wengNature ResearchViruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Matemática. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Capsid proteins often present a positively charged arginine-rich sequence at their terminal regions, which has a fundamental role in genome packaging and particle stability for some icosahedral viruses. These sequences show little to no conservation and are structurally dynamic such that they cannot be easily detected by common sequence or structure comparisons. As a result, the occurrence and distribution of positively charged domains across the viral universe are unknown. Based on the net charge calculation of discrete protein segments, we identified proteins containing amino acid stretches with a notably high net charge (Q > + 17), which are enriched in icosahedral viruses with a distinctive bias towards arginine over lysine. We used viral particle structural data to calculate the total electrostatic charge derived from the most positively charged protein segment of capsid proteins and correlated these values with genome charges arising from the phosphates of each nucleotide. We obtained a positive correlation (r = 0.91, p-value <0001) for a group of 17 viral families, corresponding to 40% of all families with icosahedral structures described to date. These data indicated that unrelated viruses with diverse genome types adopt a common underlying mechanism for capsid assembly based on R-arms.Computational biologyVirologyBioinformaticsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/40739/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALViruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdfViruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdfapplication/pdf4197378https://www.arca.fiocruz.br/bitstream/icict/40739/2/Viruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdf74c6600d05977068af2377a8466bb505MD52TEXTViruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdf.txtViruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
title Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
spellingShingle Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
Requião, Rodrigo D.
Computational biology
Virology
Bioinformatics
title_short Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
title_full Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
title_fullStr Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
title_full_unstemmed Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
title_sort Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains
author Requião, Rodrigo D.
author_facet Requião, Rodrigo D.
Carneiro, Rodolfo L.
Moreira, Mariana Hoyer
Ribeiro-Alves, Marcelo
Rossetto, Silvana
Palhano, Fernando L.
Domitrovic, Tatiana
author_role author
author2 Carneiro, Rodolfo L.
Moreira, Mariana Hoyer
Ribeiro-Alves, Marcelo
Rossetto, Silvana
Palhano, Fernando L.
Domitrovic, Tatiana
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Requião, Rodrigo D.
Carneiro, Rodolfo L.
Moreira, Mariana Hoyer
Ribeiro-Alves, Marcelo
Rossetto, Silvana
Palhano, Fernando L.
Domitrovic, Tatiana
dc.subject.en.pt_BR.fl_str_mv Computational biology
Virology
Bioinformatics
topic Computational biology
Virology
Bioinformatics
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-04-13T22:55:37Z
dc.date.available.fl_str_mv 2020-04-13T22:55:37Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REQUIÃO, Rodrigo D. et al. Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains. Scientific Reports, v. 10, n. 1, p. 1-12, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40739
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-020-62328-w
identifier_str_mv REQUIÃO, Rodrigo D. et al. Viruses with different genome types adopt a similar strategy to pack nucleic acids based on positively charged protein domains. Scientific Reports, v. 10, n. 1, p. 1-12, 2020.
2045-2322
10.1038/s41598-020-62328-w
url https://www.arca.fiocruz.br/handle/icict/40739
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/40739/3/Viruses_Marcelo_Ribeiro-Alves_etal_INI_2020.pdf.txt
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