Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII

Detalhes bibliográficos
Autor(a) principal: Aminnejad, Mojgan
Data de Publicação: 2012
Outros Autores: Diaz, Mara, Arabatzis, Michael, Castañeda, Elizabeth, Lazéra, Marcia, Velegraki, Aristea, Marriott, Deborah, Sorrell, Tania C., Meyer, Wieland
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36632
Resumo: 2020-10-22
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spelling Aminnejad, MojganDiaz, MaraArabatzis, MichaelCastañeda, ElizabethLazéra, MarciaVelegraki, AristeaMarriott, DeborahSorrell, Tania C.Meyer, Wieland2019-10-22T13:42:21Z2019-10-22T13:42:21Z2012AMINNEJAD, Mojgan et al. Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII. Mycopathologia, v. 173, p. 337-346, 2012.0301-486Xhttps://www.arca.fiocruz.br/handle/icict/3663210.1007/s11046-011-9491-x1573-0832engSpringer VerlagIdentification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGIIinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-10-22Molecular Mycology Research Laboratory. Sydney, NSW, Australia / University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia.University of Miami. Rosenstiel School of Marine and Atmospheric Sciences. Miami, FL, USA.National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.Instituto Nacional de Salud. Grupo de Microbiología. Bogotá, Colombia.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.St. Vincent’s Hospital. Department of Microbiology and Clinical Infectious Diseases. Sydney, NSW, Australia.University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia.University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia / Westmead Hospital. Molecular Mycology Research Laboratory. Sydney, NSW, Australia.Cryptococcusneoformans and Cryptococcus gattii are pathogenic yeasts causing meningoencephalitis in immunocompromised and immunocompetent hosts. The fungus is typically haploid, and sexual reproduction occurs normally between individuals with opposite mating types, a and a. C. neoformans var. grubii (serotype A) is comprised of molecular types VNI, VNII, and VNB, and C. neoformans var. neoformans (serotype D) contains the molecular type VNIV. Additionally, diploid or aneuploid AD hybrids (VNIII) have been reported. C. gattii contains the molecular types VGI, VGII, VGIII, and VGIV, which encompass both serotypes B and C. To identify possible hybrid strains, URA5-RFLP analysis was performed on 350 globally obtained clinical, environmental, and veterinary isolates. Four clinical isolates from cerebrospinal fluid showed combination patterns of C. neoformans var. grubii and C. gattii: Brazil (n = 2), Colombia (n = 1), and India (n = 1). These strains were monokaryotic and diploid or aneuploid. M13 PCR fingerprinting showed that they contained fragments of both proposed parental groups. Luminex IGS genotyping identified these isolates as hybrids with two different molecular type combinations: three VNI/VGII and one VNI/VGI. Blue color development on CGB agar was delayed in three isolates and absent in one. C. gattii-specific PCR confirmed the presence of C. gattii in the hybrids. CAP59 allele-specific PCR revealed that all the hybrids contained both serotype A and B alleles. Determination of mating-type allelic patterns by PCR revealed that the isolates were aA aB. This is the first study discovering novel natural hybrids between C. neoformans molecular type VNI and C. gattii molecular type VGII.Cryptococcus neoformansCryptococcus gattiiAB hybridsSerotypeMating typeMolecular typeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36632/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Aminnejad_Mojgan_etal_INI_2012.pdfve_Aminnejad_Mojgan_etal_INI_2012.pdfapplication/pdf370959https://www.arca.fiocruz.br/bitstream/icict/36632/2/ve_Aminnejad_Mojgan_etal_INI_2012.pdf0923ed175c5546f1380d4d2bc225578eMD52TEXTve_Aminnejad_Mojgan_etal_INI_2012.pdf.txtve_Aminnejad_Mojgan_etal_INI_2012.pdf.txtExtracted texttext/plain36534https://www.arca.fiocruz.br/bitstream/icict/36632/3/ve_Aminnejad_Mojgan_etal_INI_2012.pdf.txteaa42af00ee716c60b5bb9943abf6efdMD53icict/366322021-03-24 16:32:38.061oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
title Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
spellingShingle Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
Aminnejad, Mojgan
Cryptococcus neoformans
Cryptococcus gattii
AB hybrids
Serotype
Mating type
Molecular type
title_short Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
title_full Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
title_fullStr Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
title_full_unstemmed Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
title_sort Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII
author Aminnejad, Mojgan
author_facet Aminnejad, Mojgan
Diaz, Mara
Arabatzis, Michael
Castañeda, Elizabeth
Lazéra, Marcia
Velegraki, Aristea
Marriott, Deborah
Sorrell, Tania C.
Meyer, Wieland
author_role author
author2 Diaz, Mara
Arabatzis, Michael
Castañeda, Elizabeth
Lazéra, Marcia
Velegraki, Aristea
Marriott, Deborah
Sorrell, Tania C.
Meyer, Wieland
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aminnejad, Mojgan
Diaz, Mara
Arabatzis, Michael
Castañeda, Elizabeth
Lazéra, Marcia
Velegraki, Aristea
Marriott, Deborah
Sorrell, Tania C.
Meyer, Wieland
dc.subject.en.pt_BR.fl_str_mv Cryptococcus neoformans
Cryptococcus gattii
AB hybrids
Serotype
Mating type
Molecular type
topic Cryptococcus neoformans
Cryptococcus gattii
AB hybrids
Serotype
Mating type
Molecular type
description 2020-10-22
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-10-22T13:42:21Z
dc.date.available.fl_str_mv 2019-10-22T13:42:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AMINNEJAD, Mojgan et al. Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII. Mycopathologia, v. 173, p. 337-346, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36632
dc.identifier.issn.pt_BR.fl_str_mv 0301-486X
dc.identifier.doi.none.fl_str_mv 10.1007/s11046-011-9491-x
dc.identifier.eissn.none.fl_str_mv 1573-0832
identifier_str_mv AMINNEJAD, Mojgan et al. Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII. Mycopathologia, v. 173, p. 337-346, 2012.
0301-486X
10.1007/s11046-011-9491-x
1573-0832
url https://www.arca.fiocruz.br/handle/icict/36632
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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