Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)

Detalhes bibliográficos
Autor(a) principal: Carvalho, Isabel Maria Vicente Guedes de
Data de Publicação: 2014
Outros Autores: Queiroz, Artur Trancoso Lopo de, Moraes, Rosiane Brito de, Gil, Helio Benites, Alves, Rafael, Viviani, Andréa de Barros Pinto, Becnel, James John, Coutinho, Carlos José Pereira da Cunha de Araujo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9143
Resumo: Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil
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spelling Carvalho, Isabel Maria Vicente Guedes deQueiroz, Artur Trancoso Lopo deMoraes, Rosiane Brito deGil, Helio BenitesAlves, RafaelViviani, Andréa de Barros PintoBecnel, James JohnCoutinho, Carlos José Pereira da Cunha de Araujo2014-12-10T19:06:45Z2014-12-10T19:06:45Z2014CARVALHO, I. M. et al. Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae). Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 5, p. 624-631, 2014.0037-8682https://www.arca.fiocruz.br/handle/icict/9143dx.doi.org/10.1590/0037-8682-0165-2014engSociedade Brasileira de Medicina TropicalDescription of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório de Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, BrasilSuperintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Disciplina de Infectologia. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Departamento de Medicina. Disciplina de Gastroenterologia. São Paulo, SP, BrasilSuperintendência de Controle de Endemias. Laboratório de Simulídeos, Caraguatatuba, SP, BrasilUnited States Department of Agriculture Mosquito. Florida, USA / Fly Research Unit. Gainesville, Florida, USASuperintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, BrasilIntroduction: Microsporidia constitute the most common black fl y pathogens, although the species’ diversity, seasonal occurrence and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium (Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological characterization. Methods: Larvae were collected at a single point in a stream in a rural area of the city and were kept under artifi cial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores measuring 6.9 ± 1.0 x 5.0 ± 0.7μm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the presence of eight uninucleate spores measuring 4.5 x 3.5μm in sporophorous vesicles. Results: The molecular analysis confi rmed the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions: Samples identifi ed as Polydispyrenia spp. and Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However, the 16S gene tree showed a considerable distance between the black fl y-infecting Amblyospora spp. and the mosquito-infecting spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain a coherent phylogeny for this group.MicrosporidaeAmblyospora sppPolydispyrenia sppPhylogenetic analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9143/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALCarvalho IMVG Description....pdfCarvalho IMVG Description....pdfapplication/pdf2381980https://www.arca.fiocruz.br/bitstream/icict/9143/2/Carvalho%20IMVG%20Description....pdfb5ca55ed631a8d84dd91031fa344fdf1MD52TEXTCarvalho IMVG Description....pdf.txtCarvalho IMVG Description....pdf.txtExtracted texttext/plain32264https://www.arca.fiocruz.br/bitstream/icict/9143/3/Carvalho%20IMVG%20Description....pdf.txt38043091bca2baa623231c9acac22675MD53icict/91432023-03-15 14:33:06.052oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:06Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
title Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
spellingShingle Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
Carvalho, Isabel Maria Vicente Guedes de
Microsporidae
Amblyospora spp
Polydispyrenia spp
Phylogenetic analysis
title_short Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
title_full Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
title_fullStr Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
title_full_unstemmed Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
title_sort Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae)
author Carvalho, Isabel Maria Vicente Guedes de
author_facet Carvalho, Isabel Maria Vicente Guedes de
Queiroz, Artur Trancoso Lopo de
Moraes, Rosiane Brito de
Gil, Helio Benites
Alves, Rafael
Viviani, Andréa de Barros Pinto
Becnel, James John
Coutinho, Carlos José Pereira da Cunha de Araujo
author_role author
author2 Queiroz, Artur Trancoso Lopo de
Moraes, Rosiane Brito de
Gil, Helio Benites
Alves, Rafael
Viviani, Andréa de Barros Pinto
Becnel, James John
Coutinho, Carlos José Pereira da Cunha de Araujo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Isabel Maria Vicente Guedes de
Queiroz, Artur Trancoso Lopo de
Moraes, Rosiane Brito de
Gil, Helio Benites
Alves, Rafael
Viviani, Andréa de Barros Pinto
Becnel, James John
Coutinho, Carlos José Pereira da Cunha de Araujo
dc.subject.en.pt_BR.fl_str_mv Microsporidae
Amblyospora spp
Polydispyrenia spp
Phylogenetic analysis
topic Microsporidae
Amblyospora spp
Polydispyrenia spp
Phylogenetic analysis
description Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-12-10T19:06:45Z
dc.date.available.fl_str_mv 2014-12-10T19:06:45Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, I. M. et al. Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae). Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 5, p. 624-631, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9143
dc.identifier.issn.none.fl_str_mv 0037-8682
dc.identifier.doi.none.fl_str_mv dx.doi.org/10.1590/0037-8682-0165-2014
identifier_str_mv CARVALHO, I. M. et al. Description of microsporidia in simulids: molecular and morphological characterization of microsporidia in the larvae of Simulium pertinax Kollar (Diptera: Simuliidae). Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 5, p. 624-631, 2014.
0037-8682
dx.doi.org/10.1590/0037-8682-0165-2014
url https://www.arca.fiocruz.br/handle/icict/9143
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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