Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages

Detalhes bibliográficos
Autor(a) principal: Souza, Aline Cristina Abreu Moreira
Data de Publicação: 2015
Outros Autores: Marinho, Ygor, Correa, Gladys, Santoro, Giani França, Coutinho, Claudia Mara Lara Melo, Vommaro, Rossiane Claudia, Silva, Robson Coutinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14144
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.
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spelling Souza, Aline Cristina Abreu MoreiraMarinho, YgorCorrea, GladysSantoro, Giani FrançaCoutinho, Claudia Mara Lara MeloVommaro, Rossiane ClaudiaSilva, Robson Coutinho2016-05-10T13:37:41Z2016-05-10T13:37:41Z2015SOUZA, Aline Cristina Abreu Moreira; et al. Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages. Plos One, v.10, n.7, e0133502, 23p, Jul. 2015.https://www.arca.fiocruz.br/handle/icict/1414410.1371/journal. pone.01335021932-6203engPublic Library of SciencePyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações, Terapias, Ensino e Bioprodutos (LITEB). Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações, Terapias, Ensino e Bioprodutos (LITEB). Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.Infection by the protozoan parasite Toxoplasma gondii is highly prevalent worldwide and may have serious clinical manifestations in immunocompromised patients. T. gondii is an obligate intracellular parasite that infects almost any cell type in mammalian hosts, including immune cells. The immune cells express purinergic P2 receptors in their membrane – subdivided into P2Y and P2X subfamilies - whose activation is important for infection control. Here, we examined the effect of treatment with UTP and UDP in mouse peritoneal macrophages infected with T. gondii tachyzoites. Treatment with these nucleotides reduced parasitic load by 90%, but did not increase the levels of the inflammatory mediators NO and ROS, nor did it modulate host cell death by apoptosis or necrosis. On the other hand, UTP and UDP treatments induced early egress of tachyzoites from infected macrophages, in a Ca2+-dependent manner, as shown by scanning electron microscopy analysis, and videomicroscopy. In subsequent infections, prematurely egressed parasites had reduced infectivity, and could neither replicate nor inhibit the fusion of lysosomes to the parasitophorous vacuole. The use of selective agonists and antagonists of the receptor subtypes P2Y2 and P2Y4 and P2Y6 showed that premature parasite egress may be mediated by the activation of these receptor subtypes. Our results suggest that the activity of P2Y host cell receptors controls T. gondii infection in macrophages, highlighting the importance of pyrimidinergic signaling for innate immune system response against infection. Finally the P2Y receptors should be considered as new target for the development of drugs against T. gondii infection.Toxoplasma gondiiMacrophagesInfectionPyrimidinergic ReceptorP2Y receptorsToxoplasmaMacrófagosInfecçãoReceptoresParasitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14144/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALclaudia_coutinho_etal_IOC_2015.PDFclaudia_coutinho_etal_IOC_2015.PDFapplication/pdf8669542https://www.arca.fiocruz.br/bitstream/icict/14144/2/claudia_coutinho_etal_IOC_2015.PDF0c5ba6d6bd305f21ec2fe5247565ce03MD52TEXTclaudia_coutinho_etal_IOC_2015.PDF.txtclaudia_coutinho_etal_IOC_2015.PDF.txtExtracted 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dc.title.pt_BR.fl_str_mv Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
title Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
spellingShingle Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
Souza, Aline Cristina Abreu Moreira
Toxoplasma gondii
Macrophages
Infection
Pyrimidinergic Receptor
P2Y receptors
Toxoplasma
Macrófagos
Infecção
Receptores
Parasitos
title_short Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
title_full Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
title_fullStr Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
title_full_unstemmed Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
title_sort Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages
author Souza, Aline Cristina Abreu Moreira
author_facet Souza, Aline Cristina Abreu Moreira
Marinho, Ygor
Correa, Gladys
Santoro, Giani França
Coutinho, Claudia Mara Lara Melo
Vommaro, Rossiane Claudia
Silva, Robson Coutinho
author_role author
author2 Marinho, Ygor
Correa, Gladys
Santoro, Giani França
Coutinho, Claudia Mara Lara Melo
Vommaro, Rossiane Claudia
Silva, Robson Coutinho
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Aline Cristina Abreu Moreira
Marinho, Ygor
Correa, Gladys
Santoro, Giani França
Coutinho, Claudia Mara Lara Melo
Vommaro, Rossiane Claudia
Silva, Robson Coutinho
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
Macrophages
Infection
Pyrimidinergic Receptor
P2Y receptors
topic Toxoplasma gondii
Macrophages
Infection
Pyrimidinergic Receptor
P2Y receptors
Toxoplasma
Macrófagos
Infecção
Receptores
Parasitos
dc.subject.decs.pt_BR.fl_str_mv Toxoplasma
Macrófagos
Infecção
Receptores
Parasitos
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica (INPeTAm/UFRJ). Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-05-10T13:37:41Z
dc.date.available.fl_str_mv 2016-05-10T13:37:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Aline Cristina Abreu Moreira; et al. Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages. Plos One, v.10, n.7, e0133502, 23p, Jul. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14144
dc.identifier.doi.none.fl_str_mv 10.1371/journal. pone.0133502
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv SOUZA, Aline Cristina Abreu Moreira; et al. Pyrimidinergic Receptor Activation Controls Toxoplasma gondii Infection in Macrophages. Plos One, v.10, n.7, e0133502, 23p, Jul. 2015.
10.1371/journal. pone.0133502
1932-6203
url https://www.arca.fiocruz.br/handle/icict/14144
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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