Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Priscila Costa
Data de Publicação: 2008
Outros Autores: Nakayasu, Ernesto S., Rodrigues, Marcio L., Frases, Susana, Casadevall, Arturo, Zancope-Oliveira, Rosely M., Almeida, Igor C., Nosanchuk, Joshua D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57505
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brazil / Yeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USA / Yeshiva University. Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.
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spelling Albuquerque, Priscila CostaNakayasu, Ernesto S.Rodrigues, Marcio L.Frases, SusanaCasadevall, ArturoZancope-Oliveira, Rosely M.Almeida, Igor C.Nosanchuk, Joshua D.2023-03-23T00:59:52Z2023-03-23T00:59:52Z2008ALBUQUERQUE, Priscila Costa et al. Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. Cellular Microbiology, v. 10, n. 8, p. 1695-1710, Aug. 2008.1462-5814https://www.arca.fiocruz.br/handle/icict/5750510.1111/j.1462-5822.2008.01160.x1462-5822engWiley-BlackwellVesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brazil / Yeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USA / Yeshiva University. Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.University of Texas at El Paso. The Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ Brazil.Yeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USAYeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USA / Yeshiva University. Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brazil.University of Texas at El Paso. The Border Biomedical Research Center. Department of Biological Sciences. El Paso, TX, USA.Yeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USA / Yeshiva University. Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.Vesicular secretion of macromolecules has recently been described in the basidiomycete Cryptococcus neoformans, raising the question as to whether ascomycetes similarly utilize vesicles for transport. In the present study, we examine whether the clinically important ascomycete Histoplasma capsulatum produce vesicles and utilized these structures to secrete macromolecules. Transmission electron microscopy (TEM) shows transcellular secretion of vesicles by yeast cells. Proteomic and lipidomic analyses of vesicles isolated from culture supernatants reveal a rich collection of macromolecules involved in diverse processes, including metabolism, cell recycling, signalling and virulence. The results demonstrate that H. capsulatum can utilize a trans-cell wall vesicular transport secretory mechanism to promote virulence. Additionally, TEM of supernatants collected from Candida albicans, Candida parapsilosis, Sporothrix schenckii and Saccharomyces cerevisiae documents that vesicles are similarly produced by additional ascomycetes. The vesicles from H. capsulatum react with immune serum from patients with histoplasmosis, providing an association of the vesicular products with pathogenesis. The findings support the proposal that vesicular secretion is a general mechanism in fungi for the transport of macromolecules related to virulence and that this process could be a target for novel therapeutics.Histoplasma capsulatumAscomyceteVesiclesTranscellular transportinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Albuquerque_Priscila_etal_INI_2008.pdfve_Albuquerque_Priscila_etal_INI_2008.pdfapplication/pdf1798960https://www.arca.fiocruz.br/bitstream/icict/57505/2/ve_Albuquerque_Priscila_etal_INI_2008.pdf46ba72d0c5d5047c33b036d2dc0085deMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57505/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/575052023-03-22 22:03:15.849oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-23T01:03:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
title Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
spellingShingle Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
Albuquerque, Priscila Costa
Histoplasma capsulatum
Ascomycete
Vesicles
Transcellular transport
title_short Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
title_full Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
title_fullStr Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
title_full_unstemmed Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
title_sort Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes
author Albuquerque, Priscila Costa
author_facet Albuquerque, Priscila Costa
Nakayasu, Ernesto S.
Rodrigues, Marcio L.
Frases, Susana
Casadevall, Arturo
Zancope-Oliveira, Rosely M.
Almeida, Igor C.
Nosanchuk, Joshua D.
author_role author
author2 Nakayasu, Ernesto S.
Rodrigues, Marcio L.
Frases, Susana
Casadevall, Arturo
Zancope-Oliveira, Rosely M.
Almeida, Igor C.
Nosanchuk, Joshua D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Albuquerque, Priscila Costa
Nakayasu, Ernesto S.
Rodrigues, Marcio L.
Frases, Susana
Casadevall, Arturo
Zancope-Oliveira, Rosely M.
Almeida, Igor C.
Nosanchuk, Joshua D.
dc.subject.en.en_US.fl_str_mv Histoplasma capsulatum
Ascomycete
Vesicles
Transcellular transport
topic Histoplasma capsulatum
Ascomycete
Vesicles
Transcellular transport
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brazil / Yeshiva University. Albert Einstein College of Medicine. Division of Infectious Diseases. Department of Microbiology and Immunology. New York, NY, USA / Yeshiva University. Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2023-03-23T00:59:52Z
dc.date.available.fl_str_mv 2023-03-23T00:59:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALBUQUERQUE, Priscila Costa et al. Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. Cellular Microbiology, v. 10, n. 8, p. 1695-1710, Aug. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57505
dc.identifier.issn.en_US.fl_str_mv 1462-5814
dc.identifier.doi.none.fl_str_mv 10.1111/j.1462-5822.2008.01160.x
dc.identifier.eissn.none.fl_str_mv 1462-5822
identifier_str_mv ALBUQUERQUE, Priscila Costa et al. Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. Cellular Microbiology, v. 10, n. 8, p. 1695-1710, Aug. 2008.
1462-5814
10.1111/j.1462-5822.2008.01160.x
1462-5822
url https://www.arca.fiocruz.br/handle/icict/57505
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57505/2/ve_Albuquerque_Priscila_etal_INI_2008.pdf
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