The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin

Detalhes bibliográficos
Autor(a) principal: Silva Neto, Benedito Rodrigues da
Data de Publicação: 2009
Outros Autores: Silva, Julhiany de Fátima da, Giannini, Maria José Soares Mendes, Lenzi, Henrique Leonel, Soares, Célia Maria de Almeida, Pereira, Maristela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3735
Resumo: This study at the Universidade Federal de Goiás was supported by grants from the Ministério de Ciência e Tecnologia (MCT), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), and by the International Foundation for Science (IFS), Stockholm, Sweden, through a grant to M.P.. B.R.S.N. was supported by a fellowship from Coordenação de Aperfeiçoamento de Ensino Superior (CAPES).
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spelling Silva Neto, Benedito Rodrigues daSilva, Julhiany de Fátima daGiannini, Maria José Soares MendesLenzi, Henrique LeonelSoares, Célia Maria de AlmeidaPereira, Maristela2012-01-16T16:59:36Z2012-01-16T16:59:36Z2009SILVA NETO, Benedito Rodrigues da et al. The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesion. BMC Microbiology, v. 9, n. 272, 2009.1471-2180https://www.arca.fiocruz.br/handle/icict/373510.1186/1471-2180-9-272This study at the Universidade Federal de Goiás was supported by grants from the Ministério de Ciência e Tecnologia (MCT), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), and by the International Foundation for Science (IFS), Stockholm, Sweden, through a grant to M.P.. B.R.S.N. was supported by a fellowship from Coordenação de Aperfeiçoamento de Ensino Superior (CAPES).Universidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular, Goiânia, GO, BrasilUniversidade Estadual Paulista. Laboratório de Micologia Clínica. Araraquara, SP, BrasilUniversidade Estadual Paulista. Laboratório de Micologia Clínica. Araraquara, SP, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, BrasilUniversidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular, Goiânia, GO, BrasilUniversidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular, Goiânia, GO, BrasilBackground: The pathogenic fungus Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM). This is a pulmonary mycosis acquired by inhalation of fungal airborne propagules that can disseminate to several organs and tissues leading to a severe form of the disease. Adhesion and invasion to host cells are essential steps involved in the internalization and dissemination of pathogens. Inside the host, P. brasiliensis may use the glyoxylate cycle for intracellular survival. Results: Here, we provide evidence that the malate synthase of P. brasiliensis (PbMLS) is located on the fungal cell surface, and is secreted. PbMLS was overexpressed in Escherichia coli, and polyclonal antibody was obtained against this protein. By using Confocal Laser Scanning Microscopy, PbMLS was detected in the cytoplasm and in the cell wall of the mother, but mainly of budding cells of the P. brasiliensis yeast phase. PbMLSr and its respective polyclonal antibody produced against this protein inhibited the interaction of P. brasiliensis with in vitro cultured epithelial cells A549. Conclusion: These observations indicated that cell wall-associated PbMLS could be mediating the binding of fungal cells to the host, thus contributing to the adhesion of fungus to host tissues and to the dissemination of infection, behaving as an anchorless adhesin.engBioMedCentralThe malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParacoccidioides brasiliensisGlyoxylate cycleMalate synthaseAnchorlessAdhesinsParacoccidioides brasiliensisMalato SintaseAdesão Celularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALThe malate synthase of Paracoccidioides brasiliensis.pdfThe malate synthase of Paracoccidioides brasiliensis.pdfapplication/pdf2830627https://www.arca.fiocruz.br/bitstream/icict/3735/1/The%20malate%20synthase%20of%20Paracoccidioides%20brasiliensis.pdf0c7455b8252aa62fc1be0f88aa8c7d95MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3735/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTThe malate synthase of Paracoccidioides brasiliensis.pdf.txtThe malate synthase of Paracoccidioides brasiliensis.pdf.txtExtracted texttext/plain53135https://www.arca.fiocruz.br/bitstream/icict/3735/5/The%20malate%20synthase%20of%20Paracoccidioides%20brasiliensis.pdf.txte328cf4448342ee34ae938f9286c1ac4MD55THUMBNAILThe malate synthase of Paracoccidioides brasiliensis.pdf.jpgThe malate synthase of Paracoccidioides brasiliensis.pdf.jpgGenerated Thumbnailimage/jpeg2055https://www.arca.fiocruz.br/bitstream/icict/3735/4/The%20malate%20synthase%20of%20Paracoccidioides%20brasiliensis.pdf.jpg11a941dbb503c60997e9dcc8abd2b1cdMD54icict/37352018-04-06 08:58:03.213oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:58:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
title The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
spellingShingle The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
Silva Neto, Benedito Rodrigues da
Paracoccidioides brasiliensis
Glyoxylate cycle
Malate synthase
Anchorless
Adhesins
Paracoccidioides brasiliensis
Malato Sintase
Adesão Celular
title_short The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
title_full The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
title_fullStr The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
title_full_unstemmed The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
title_sort The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
author Silva Neto, Benedito Rodrigues da
author_facet Silva Neto, Benedito Rodrigues da
Silva, Julhiany de Fátima da
Giannini, Maria José Soares Mendes
Lenzi, Henrique Leonel
Soares, Célia Maria de Almeida
Pereira, Maristela
author_role author
author2 Silva, Julhiany de Fátima da
Giannini, Maria José Soares Mendes
Lenzi, Henrique Leonel
Soares, Célia Maria de Almeida
Pereira, Maristela
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva Neto, Benedito Rodrigues da
Silva, Julhiany de Fátima da
Giannini, Maria José Soares Mendes
Lenzi, Henrique Leonel
Soares, Célia Maria de Almeida
Pereira, Maristela
dc.subject.en.pt_BR.fl_str_mv Paracoccidioides brasiliensis
Glyoxylate cycle
Malate synthase
Anchorless
Adhesins
topic Paracoccidioides brasiliensis
Glyoxylate cycle
Malate synthase
Anchorless
Adhesins
Paracoccidioides brasiliensis
Malato Sintase
Adesão Celular
dc.subject.decs.pt_BR.fl_str_mv Paracoccidioides brasiliensis
Malato Sintase
Adesão Celular
description This study at the Universidade Federal de Goiás was supported by grants from the Ministério de Ciência e Tecnologia (MCT), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), and by the International Foundation for Science (IFS), Stockholm, Sweden, through a grant to M.P.. B.R.S.N. was supported by a fellowship from Coordenação de Aperfeiçoamento de Ensino Superior (CAPES).
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2012-01-16T16:59:36Z
dc.date.available.fl_str_mv 2012-01-16T16:59:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA NETO, Benedito Rodrigues da et al. The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesion. BMC Microbiology, v. 9, n. 272, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3735
dc.identifier.issn.none.fl_str_mv 1471-2180
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2180-9-272
identifier_str_mv SILVA NETO, Benedito Rodrigues da et al. The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesion. BMC Microbiology, v. 9, n. 272, 2009.
1471-2180
10.1186/1471-2180-9-272
url https://www.arca.fiocruz.br/handle/icict/3735
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dc.publisher.none.fl_str_mv BioMedCentral
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