Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene

Detalhes bibliográficos
Autor(a) principal: Almeida, Dalziza Victalina de
Data de Publicação: 2016
Outros Autores: Macieira, Karine Venegas, Grinsztejn, Beatriz, Santos, Valdiléa Gonçalves Veloso Dos, Guimarães, Monick Lindenmeyer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17885
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.
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spelling Almeida, Dalziza Victalina deMacieira, Karine VenegasGrinsztejn, BeatrizSantos, Valdiléa Gonçalves Veloso DosGuimarães, Monick Lindenmeyer2017-02-23T11:45:47Z2017-02-23T11:45:47Z2016ALMEIDA, Dalziza Victalina de; et al. Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene. PLoS ONE, v.11, n.12, e0167690, 16p, Dec. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1788510.1371/journal.pone.0167690engPublic Library of ScienceAnticorposHIV-1Características genéticas e bioquímicasHIV-1env GeneCross-Neutralizing AntibodiesGenetics and Biochemical CharacteristicsCross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Various HIV-1 env genetic and biochemical features impact the elicitation of cross-reactive neutralizing antibodies in natural infections. Thus, we aimed to investigate cross-neutralizing antibodies in individuals infected with HIV-1 env subtypes B, F1, C or the B/Bbr variant as well as env characteristics. Therefore, plasma samples from Brazilian chronically HIV-1 infected individuals were submitted to the TZM-bl neutralization assay. We also analyzed putative N-glycosylation sites (PNGLs) and the size of gp120 variable domains in the context of HIV-1 subtypes prevalent in Brazil. We observed a greater breadth and potency of the anti-Env neutralizing response in individuals infected with the F1 or B HIV-1 subtypes compared with the C subtype and the variant B/Bbr. We observed greater V1 B/Bbr and smaller V4 F1 than those of other subtypes (p<0.005), however neither was there a correlation verified between the variable region length and neutralization potency, nor between PNLG and HIV-1 subtypes. The enrichment of W at top of V3 loop in weak neutralizing response viruses and the P in viruses with higher neutralization susceptibility was statistically significant (p = 0.013). Some other signatures sites were associated to HIV-1 subtype-specific F1 and B/Bbr samples might influence in the distinct neutralizing response. These results indicate that a single amino acid substitution may lead to a distinct conformational exposure or load in the association domain of the trimer of gp120 and interfere with the induction power of the neutralizing response, which affects the sensitivity of the neutralizing antibody and has significant implications for vaccine design.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17885/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdalziza_almeida_etal_IOC_2016.pdfdalziza_almeida_etal_IOC_2016.pdfapplication/pdf2273563https://www.arca.fiocruz.br/bitstream/icict/17885/2/dalziza_almeida_etal_IOC_2016.pdf20cd37b2c6850c599cbddfbb6873d184MD52TEXTdalziza_almeida_etal_IOC_2016.pdf.txtdalziza_almeida_etal_IOC_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
title Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
spellingShingle Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
Almeida, Dalziza Victalina de
Anticorpos
HIV-1
Características genéticas e bioquímicas
HIV-1
env Gene
Cross-Neutralizing Antibodies
Genetics and Biochemical Characteristics
title_short Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
title_full Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
title_fullStr Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
title_full_unstemmed Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
title_sort Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene
author Almeida, Dalziza Victalina de
author_facet Almeida, Dalziza Victalina de
Macieira, Karine Venegas
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso Dos
Guimarães, Monick Lindenmeyer
author_role author
author2 Macieira, Karine Venegas
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso Dos
Guimarães, Monick Lindenmeyer
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Dalziza Victalina de
Macieira, Karine Venegas
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso Dos
Guimarães, Monick Lindenmeyer
dc.subject.other.pt_BR.fl_str_mv Anticorpos
HIV-1
Características genéticas e bioquímicas
topic Anticorpos
HIV-1
Características genéticas e bioquímicas
HIV-1
env Gene
Cross-Neutralizing Antibodies
Genetics and Biochemical Characteristics
dc.subject.en.pt_BR.fl_str_mv HIV-1
env Gene
Cross-Neutralizing Antibodies
Genetics and Biochemical Characteristics
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-23T11:45:47Z
dc.date.available.fl_str_mv 2017-02-23T11:45:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Dalziza Victalina de; et al. Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene. PLoS ONE, v.11, n.12, e0167690, 16p, Dec. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17885
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0167690
identifier_str_mv ALMEIDA, Dalziza Victalina de; et al. Cross-Neutralizing Antibodies in HIV-1 Individuals Infected by Subtypes B, F1, C or the B/Bbr Variant in Relation to the Genetics and Biochemical Characteristics of the env Gene. PLoS ONE, v.11, n.12, e0167690, 16p, Dec. 2016.
1932-6203
10.1371/journal.pone.0167690
url https://www.arca.fiocruz.br/handle/icict/17885
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17885/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/17885/2/dalziza_almeida_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/17885/3/dalziza_almeida_etal_IOC_2016.pdf.txt
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