3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures

Detalhes bibliográficos
Autor(a) principal: Biagini, Giuliana
Data de Publicação: 2021
Outros Autores: Senegaglia, Alexandra Cristina, Pereira, Tarciso, Berti, Lucas Freitas, Marcon, Bruna Hilzendeger, Stimamiglio, Marco Augusto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fbioe.2021.700862
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49809
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Biagini, GiulianaSenegaglia, Alexandra CristinaPereira, TarcisoBerti, Lucas FreitasMarcon, Bruna HilzendegerStimamiglio, Marco Augusto2021-11-12T13:42:24Z2021-11-12T13:42:24Z2021BIAGINI, Giuliana et al. 3D Poly(Lactic Acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures. Front. Bioeng. Biotechnol. v.9, n. 700862 p. 1–15, 2021.2296-4185https://www.arca.fiocruz.br/handle/icict/4980910.3389/fbioe.2021.700862porFrontiers MediaCélulas CD133 +Mesenchymal Stem CellsTissue EngineeringTissue ScaffoldsPoly(lactic acid)–PLACélulas Madre MesenquimatosasIngeniería de TejidosAndamios del TejidoCopolímero de Ácido Poliláctico-Ácido PoliglicólicoCellules souches mésenchymateusesIngénierie tissulaireStructures d'échafaudage tissulairesCopolymère d'acide poly(lactique-co-glycolique)Células-Tronco MesenquimaisEngenharia TecidualTecidos SuporteCopolímero de Ácido Poliláctico e Ácido Poliglicólico3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D culturesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Escola de Medicina. Programa de Graduação em Ciências da Saúde. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Universidade Tecnológica Federal do Paraná. Departamento de Engenharia Mecânica. Programa de Pós-Graduação em Engenharia Biomédica. Curitiba, PR, Brasil.Universidade Tecnológica Federal do Paraná. Departamento de Engenharia Mecânica. Programa de Pós-Graduação em Engenharia Biomédica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Tissue engineering is a branch of regenerative medicine, which comprises the combination of biomaterials, cells and other bioactive molecules to regenerate tissues. Biomaterial scaffolds act as substrate and as physical support for cells and they can also reproduce the extracellular matrix cues. Although tissue engineering applications in cellular therapy tend to focus on the use of specialized cells from particular tissues or stem cells, little attention has been paid to endothelial progenitors, an important cell type in tissue regeneration. We combined 3D printed poly(lactic acid) scaffolds comprising two different pore sizes with human adipose-derived stromal cells (hASCs) and expanded CD133+ cells to evaluate how these two cell types respond to the different architectures. hASCs represent an ideal source of cells for tissue engineering applications due to their low immunogenicity, paracrine activity and ability to differentiate. Expanded CD133+ cells were isolated from umbilical cord blood and represent a source of endothelial-like cells with angiogenic potential. Fluorescence microscopy and scanning electron microscopy showed that both cell types were able to adhere to the scaffolds and maintain their characteristic morphologies. The porous PLA scaffolds stimulated cell cycle progression of hASCs but led to an arrest in the G1 phase and reduced proliferation of expanded CD133+ cells. Also, while hASCs maintained their undifferentiated profile after 7 days of culture on the scaffolds, expanded CD133+ cells presented a reduction of the von Willebrand factor (vWF), which affected the cells’ angiogenic potential. We did not observe changes in cell behavior for any of the parameters analyzed between the scaffolds with different pore sizes, but the 3D environment created by the scaffolds had different effects on the cell types tested. Unlike the extensively used mesenchymal stem cell types, the 3D PLA scaffolds led to opposite behaviors of the expanded CD133+ cells in terms of cytotoxicity, proliferation and immunophenotype. The results obtained reinforce the importance of studying how different cell types respond to 3D culture systems when considering the scaffold approach for tissue engineering.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/49809/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALfbioe-09-700862ok.pdffbioe-09-700862ok.pdfapplication/pdf3144765https://www.arca.fiocruz.br/bitstream/icict/49809/2/fbioe-09-700862ok.pdf1d9e85d7b02dfd1b25b79fa1cd66de58MD52icict/498092021-11-12 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dc.title.pt_BR.fl_str_mv 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
title 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
spellingShingle 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
Biagini, Giuliana
Células CD133 +
Mesenchymal Stem Cells
Tissue Engineering
Tissue Scaffolds
Poly(lactic acid)–PLA
Células Madre Mesenquimatosas
Ingeniería de Tejidos
Andamios del Tejido
Copolímero de Ácido Poliláctico-Ácido Poliglicólico
Cellules souches mésenchymateuses
Ingénierie tissulaire
Structures d'échafaudage tissulaires
Copolymère d'acide poly(lactique-co-glycolique)
Células-Tronco Mesenquimais
Engenharia Tecidual
Tecidos Suporte
Copolímero de Ácido Poliláctico e Ácido Poliglicólico
title_short 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
title_full 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
title_fullStr 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
title_full_unstemmed 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
title_sort 3D poly(lactic acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures
author Biagini, Giuliana
author_facet Biagini, Giuliana
Senegaglia, Alexandra Cristina
Pereira, Tarciso
Berti, Lucas Freitas
Marcon, Bruna Hilzendeger
Stimamiglio, Marco Augusto
author_role author
author2 Senegaglia, Alexandra Cristina
Pereira, Tarciso
Berti, Lucas Freitas
Marcon, Bruna Hilzendeger
Stimamiglio, Marco Augusto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Biagini, Giuliana
Senegaglia, Alexandra Cristina
Pereira, Tarciso
Berti, Lucas Freitas
Marcon, Bruna Hilzendeger
Stimamiglio, Marco Augusto
dc.subject.other.pt_BR.fl_str_mv Células CD133 +
topic Células CD133 +
Mesenchymal Stem Cells
Tissue Engineering
Tissue Scaffolds
Poly(lactic acid)–PLA
Células Madre Mesenquimatosas
Ingeniería de Tejidos
Andamios del Tejido
Copolímero de Ácido Poliláctico-Ácido Poliglicólico
Cellules souches mésenchymateuses
Ingénierie tissulaire
Structures d'échafaudage tissulaires
Copolymère d'acide poly(lactique-co-glycolique)
Células-Tronco Mesenquimais
Engenharia Tecidual
Tecidos Suporte
Copolímero de Ácido Poliláctico e Ácido Poliglicólico
dc.subject.en.pt_BR.fl_str_mv Mesenchymal Stem Cells
Tissue Engineering
Tissue Scaffolds
Poly(lactic acid)–PLA
dc.subject.es.pt_BR.fl_str_mv Células Madre Mesenquimatosas
Ingeniería de Tejidos
Andamios del Tejido
Copolímero de Ácido Poliláctico-Ácido Poliglicólico
dc.subject.fr.pt_BR.fl_str_mv Cellules souches mésenchymateuses
Ingénierie tissulaire
Structures d'échafaudage tissulaires
Copolymère d'acide poly(lactique-co-glycolique)
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco Mesenquimais
Engenharia Tecidual
Tecidos Suporte
Copolímero de Ácido Poliláctico e Ácido Poliglicólico
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-12T13:42:24Z
dc.date.available.fl_str_mv 2021-11-12T13:42:24Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BIAGINI, Giuliana et al. 3D Poly(Lactic Acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures. Front. Bioeng. Biotechnol. v.9, n. 700862 p. 1–15, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49809
dc.identifier.issn.pt_BR.fl_str_mv 2296-4185
dc.identifier.doi.none.fl_str_mv 10.3389/fbioe.2021.700862
identifier_str_mv BIAGINI, Giuliana et al. 3D Poly(Lactic Acid) scaffolds promote different behaviors on endothelial progenitors and adipose-derived stromal cells in comparison with standard 2D cultures. Front. Bioeng. Biotechnol. v.9, n. 700862 p. 1–15, 2021.
2296-4185
10.3389/fbioe.2021.700862
url https://www.arca.fiocruz.br/handle/icict/49809
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49809/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49809/2/fbioe-09-700862ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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