Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42520 |
Resumo: | Temple University. Biology Department/Institute of Genomics and Evolutionary Medicine. Philadelphia, PA, USA. |
| id |
CRUZ_dffe988400a1bfe25c0655d9d3ee90cf |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/42520 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Pacheco, M. AndreínaForero-Peña, David A.Schneider, Kristan A.Chavero, MelynarGamardo, AngelFiguera, LuisamyKadakia, Esha R.Grillet, María E.Ferreira, Joseli OliveiraEscalante, Ananias A.2020-08-03T20:28:56Z2020-08-03T20:28:56Z2020PACHECO, M. Andreína et al. Malaria in Venezuela: changes in the complexity of infection refects the increment in transmission intensity. Malaria Journal, v. 19, n. 176, p. 1-15, 2020.1475-2875https://www.arca.fiocruz.br/handle/icict/4252010.1186/s12936-020-03247-zengBMCGenes de resistência a drogasMicrossatélitesMultiplicidade de infecçãoPlasmodium falciparum,Plasmodium vivaxIntensidade de transmissãoVenezuelaDrug resistance genesPfdhpsPfdhfPfk13 geneMicrosatellitesMultiplicity of infectionPlasmodium falciparum,Plasmodium vivaxTransmission intensityVenezuelaMalaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTemple University. Biology Department/Institute of Genomics and Evolutionary Medicine. Philadelphia, PA, USA.Escuela de Ciencias de la Salud. Universidad de Oriente. Núcleo Bolívar. Ciudad Bolívar, Venezuela / Complejo Hospitalario Universitario “Ruíz y Páez”. Departamento de Medicina Interna. Ciudad Bolívar, Venezuela / Biomedical Research and Therapeutic Vaccines Institute. Ciudad Bolívar, Venezuela.University of Applied Sciences Mittweida. Department CB. Mittweida, Germany.Escuela de Ciencias de la Salud. Universidad de Oriente. Núcleo Bolívar. Ciudad Bolívar, Venezuela / Complejo Hospitalario Universitario “Ruíz y Páez”. Departamento de Medicina Interna. Ciudad Bolívar, Venezuela / Biomedical Research and Therapeutic Vaccines Institute. Ciudad Bolívar, Venezuela.Biomedical Research and Therapeutic Vaccines Institute. Ciudad Bolívar, Venezuela.Complejo Hospitalario Universitario “Ruíz y Páez”. Departamento de Medicina Interna. Ciudad Bolívar, Venezuela / Biomedical Research and Therapeutic Vaccines Institute. Ciudad Bolívar, Venezuela.Temple University. Biology Department. Institute of Genomics and Evolutionary Medicine. Philadelphia, PA, USA.Universidad Central de Venezuela. Instituto de Zoología y Ecología Tropical. Caracas, Venezuela.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Temple University. Biology Department/Institute of Genomics and Evolutionary Medicine. Philadelphia, PA, USA.Background: Malaria incidence has reached staggering numbers in Venezuela. Commonly, Bolívar State accounted for approximately 70% of the country cases every year. Most cases cluster in the Sifontes municipality, a region characterized by an extractive economy, including gold mining. An increase in migration to Sifontes, driven by gold mining, fueled a malaria spillover to the rest of the country and the region. Here samples collected in 2018 were compared with a previous study of 2003/2004 to describe changes in the parasites population structures and the frequency of point mutations linked to anti-malarial drugs. Methods: A total of 88 Plasmodium falciparum and 94 Plasmodium vivax isolates were collected in 2018 and compared with samples from 2003/2004 (106 P. falciparum and 104 P. vivax). For P. falciparum, mutations linked to drug resistance (Pfdhfr, Pfdhps, and Pfcrt) and the Pfk13 gene associated with artemisinin delayed parasite clearance, were analysed. To estimate the multiplicity of infection (MOI), and perform P. falciparum and P. vivax population genetic analyses, the parasites were genotyped by using eight standardized microsatellite loci. Results: The P. falciparum parasites are still harbouring drug-resistant mutations in Pfdhfr, Pfdhps, and Pfcrt. However, there was a decrease in the frequency of highly resistant Pfdhps alleles. Mutations associated with artemisinin delayed parasite clearance in the Pfk13 gene were not found. Consistent with the increase in transmission, polyclonal infections raised from 1.9% in 2003/2004 to 39% in 2018 in P. falciparum and from 16.3 to 68% in P. vivax. There is also a decrease in linkage disequilibrium. Bayesian clustering yields two populations linked to the time of sampling, showing that the parasite populations temporarily changed. However, the samples from 2003/2004 and 2018 have several alleles per locus in common without sharing multi-locus genotypes. Conclusions: The frequency of mutations linked with drug resistance in P. falciparum shows only changes in Pfdhps. Observations presented here are consistent with an increase in transmission from the previously circulating parasites. Following populations longitudinally, using molecular surveillance, provides valuable information in cases such as Venezuela with a fuid malaria situation that is afecting the regional goals toward elimination.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42520/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJoseliOliveira_Fereira_etal_IOC_2020.pdfJoseliOliveira_Fereira_etal_IOC_2020.pdfapplication/pdf4265806https://www.arca.fiocruz.br/bitstream/icict/42520/2/JoseliOliveira_Fereira_etal_IOC_2020.pdf30629f1ac76a43830cba3b1e0b90273fMD52TEXTJoseliOliveira_Fereira_etal_IOC_2020.pdf.txtJoseliOliveira_Fereira_etal_IOC_2020.pdf.txtExtracted texttext/plain63551https://www.arca.fiocruz.br/bitstream/icict/42520/3/JoseliOliveira_Fereira_etal_IOC_2020.pdf.txte25a6de0b0fc8a8352d8749e95fea42dMD53icict/425202020-09-29 21:48:07.045oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-09-30T00:48:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| title |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| spellingShingle |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity Pacheco, M. Andreína Genes de resistência a drogas Microssatélites Multiplicidade de infecção Plasmodium falciparum, Plasmodium vivax Intensidade de transmissão Venezuela Drug resistance genes Pfdhps Pfdhf Pfk13 gene Microsatellites Multiplicity of infection Plasmodium falciparum, Plasmodium vivax Transmission intensity Venezuela |
| title_short |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| title_full |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| title_fullStr |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| title_full_unstemmed |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| title_sort |
Malaria in Venezuela: changes in the complexity of infection reflects the increment in transmission intensity |
| author |
Pacheco, M. Andreína |
| author_facet |
Pacheco, M. Andreína Forero-Peña, David A. Schneider, Kristan A. Chavero, Melynar Gamardo, Angel Figuera, Luisamy Kadakia, Esha R. Grillet, María E. Ferreira, Joseli Oliveira Escalante, Ananias A. |
| author_role |
author |
| author2 |
Forero-Peña, David A. Schneider, Kristan A. Chavero, Melynar Gamardo, Angel Figuera, Luisamy Kadakia, Esha R. Grillet, María E. Ferreira, Joseli Oliveira Escalante, Ananias A. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pacheco, M. Andreína Forero-Peña, David A. Schneider, Kristan A. Chavero, Melynar Gamardo, Angel Figuera, Luisamy Kadakia, Esha R. Grillet, María E. Ferreira, Joseli Oliveira Escalante, Ananias A. |
| dc.subject.other.pt_BR.fl_str_mv |
Genes de resistência a drogas Microssatélites Multiplicidade de infecção Plasmodium falciparum, Plasmodium vivax Intensidade de transmissão Venezuela |
| topic |
Genes de resistência a drogas Microssatélites Multiplicidade de infecção Plasmodium falciparum, Plasmodium vivax Intensidade de transmissão Venezuela Drug resistance genes Pfdhps Pfdhf Pfk13 gene Microsatellites Multiplicity of infection Plasmodium falciparum, Plasmodium vivax Transmission intensity Venezuela |
| dc.subject.en.pt_BR.fl_str_mv |
Drug resistance genes Pfdhps Pfdhf Pfk13 gene Microsatellites Multiplicity of infection Plasmodium falciparum, Plasmodium vivax Transmission intensity Venezuela |
| description |
Temple University. Biology Department/Institute of Genomics and Evolutionary Medicine. Philadelphia, PA, USA. |
| publishDate |
2020 |
| dc.date.accessioned.fl_str_mv |
2020-08-03T20:28:56Z |
| dc.date.available.fl_str_mv |
2020-08-03T20:28:56Z |
| dc.date.issued.fl_str_mv |
2020 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
PACHECO, M. Andreína et al. Malaria in Venezuela: changes in the complexity of infection refects the increment in transmission intensity. Malaria Journal, v. 19, n. 176, p. 1-15, 2020. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/42520 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1475-2875 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s12936-020-03247-z |
| identifier_str_mv |
PACHECO, M. Andreína et al. Malaria in Venezuela: changes in the complexity of infection refects the increment in transmission intensity. Malaria Journal, v. 19, n. 176, p. 1-15, 2020. 1475-2875 10.1186/s12936-020-03247-z |
| url |
https://www.arca.fiocruz.br/handle/icict/42520 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BMC |
| publisher.none.fl_str_mv |
BMC |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/42520/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/42520/2/JoseliOliveira_Fereira_etal_IOC_2020.pdf https://www.arca.fiocruz.br/bitstream/icict/42520/3/JoseliOliveira_Fereira_etal_IOC_2020.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 30629f1ac76a43830cba3b1e0b90273f e25a6de0b0fc8a8352d8749e95fea42d |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324843870945280 |