Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Luz, Zélia Maria Profeta da
Data de Publicação: 2009
Outros Autores: Silva, Alexandre Rotondo da, Silva, Fernanda de Oliveira, Caligiorne, Rachel Basques, Oliveira, Edward José de, Rabello, Ana Lúcia Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3255
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.
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spelling Luz, Zélia Maria Profeta daSilva, Alexandre Rotondo daSilva, Fernanda de OliveiraCaligiorne, Rachel BasquesOliveira, Edward José deRabello, Ana Lúcia Teles2011-10-08T01:31:07Z2011-10-08T01:31:07Z2009LUZ, Zélia Maria Profeta et al. Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 1, p. 62-66, 2009.https://www.arca.fiocruz.br/handle/icict/3255engFundação Oswaldo Cruz.Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.The detection of Leishmania spp. in skin lesion aspirates, using a puncture technique, was evaluated in 76 patients with cutaneous leishmaniasis (CL) who were referred to a Leishmaniasis Reference Centre in Brazil. CL was defined based on skin lesions suggestive of the disease and on a positive result of the Montenegro skin test or Giemsa-stained imprints of biopsy ragments. The aspirates were cultured using a vacuum tube device containing culture medium and evaluated for the presence of Leishmania spp. The biphasic medium culture was examined once a week for three weeks. Promastigotes were observed in 53/76 (69.7%) cultures. Stained smears from 60 of the 76 patients were evaluated using PCR-RFLP to detect the conserved minicircle region of Leishmania spp. and to classify the arasite. Of these patients, 45 (75%) showed positive results in aspirate culture and 15 presented negative results. The PCR was positive in 80% (53/60) samples. The PCR-RFLP profile was determined in 49 samples, of which 45 (92%) showed a pattern compatible with Leishmania (Viannia) braziliensis. The aspirate culture is a sensitive and feasible method for diagnosing CL and may be routinely adopted by health services for L. (V.) braziliensis isolation and identification.Puncture biopsyCutaneous leishmaniasisDiagnosisHealth servicesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3255/2/license.txte095249ac7cacefbfe39684dfe45e706MD52ORIGINALLesion_aspirate_culture.pdfLesion_aspirate_culture.pdfapplication/pdf310817https://www.arca.fiocruz.br/bitstream/icict/3255/3/Lesion_aspirate_culture.pdf3168cb2593b4313804b7487c5fc188e1MD53TEXTLesion_aspirate_culture.pdf.txtLesion_aspirate_culture.pdf.txtExtracted texttext/plain26693https://www.arca.fiocruz.br/bitstream/icict/3255/6/Lesion_aspirate_culture.pdf.txtdf22213b920029cffa7c46e413e28a95MD56THUMBNAILLesion_aspirate_culture.pdf.jpgLesion_aspirate_culture.pdf.jpgGenerated Thumbnailimage/jpeg2422https://www.arca.fiocruz.br/bitstream/icict/3255/5/Lesion_aspirate_culture.pdf.jpg0b2df4db9d35e181da02135f59782ad0MD55icict/32552018-05-02 19:58:47.181oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-02T22:58:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
title Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
spellingShingle Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
Luz, Zélia Maria Profeta da
Puncture biopsy
Cutaneous leishmaniasis
Diagnosis
Health services
title_short Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
title_full Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
title_fullStr Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
title_full_unstemmed Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
title_sort Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis
author Luz, Zélia Maria Profeta da
author_facet Luz, Zélia Maria Profeta da
Silva, Alexandre Rotondo da
Silva, Fernanda de Oliveira
Caligiorne, Rachel Basques
Oliveira, Edward José de
Rabello, Ana Lúcia Teles
author_role author
author2 Silva, Alexandre Rotondo da
Silva, Fernanda de Oliveira
Caligiorne, Rachel Basques
Oliveira, Edward José de
Rabello, Ana Lúcia Teles
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Luz, Zélia Maria Profeta da
Silva, Alexandre Rotondo da
Silva, Fernanda de Oliveira
Caligiorne, Rachel Basques
Oliveira, Edward José de
Rabello, Ana Lúcia Teles
dc.subject.en.pt_BR.fl_str_mv Puncture biopsy
Cutaneous leishmaniasis
Diagnosis
Health services
topic Puncture biopsy
Cutaneous leishmaniasis
Diagnosis
Health services
description Fundação Oswaldo Cruz. Instituto René Rachou. Centro de Referência em Leishmanioses. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2011-10-08T01:31:07Z
dc.date.available.fl_str_mv 2011-10-08T01:31:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv LUZ, Zélia Maria Profeta et al. Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 1, p. 62-66, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3255
identifier_str_mv LUZ, Zélia Maria Profeta et al. Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 1, p. 62-66, 2009.
url https://www.arca.fiocruz.br/handle/icict/3255
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz.
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