Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees

Detalhes bibliográficos
Autor(a) principal: Santos, Ana Carolina Costa
Data de Publicação: 2023
Outros Autores: Borges, Luiza Diniz Ferreira, Rocha, Nina Dias Coelho, Azevedo, Vasco Ariston de Carvalho, Bonetti, Ana Maria, Santos, Anderson Rodrigues Dos, Fernandes, Gabriel da Rocha, Dantas, Raquel Cristina Cavalcanti, Ueira-Vieira, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57945
Resumo: 2030-12-31
id CRUZ_e172b76d3d6c5f97637017e4831e825f
oai_identifier_str oai:www.arca.fiocruz.br:icict/57945
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Santos, Ana Carolina CostaBorges, Luiza Diniz FerreiraRocha, Nina Dias CoelhoAzevedo, Vasco Ariston de CarvalhoBonetti, Ana MariaSantos, Anderson Rodrigues DosFernandes, Gabriel da RochaDantas, Raquel Cristina CavalcantiUeira-Vieira, Carlos2023-04-24T17:54:54Z2023-04-24T17:54:54Z2023SANTOS, Ana Carolina Costa et al. Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees. Sci Rep, v. 13, n. 1, 5147, 2023. doi: 10.1038/s41598-023-32298-w.2045-2322https://www.arca.fiocruz.br/handle/icict/57945engNature Publishing GroupBacteria, yeasts, and fungi associated with larval food of Brazilian native stingless beesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2030-12-31Laboratory of Genetics. Institute of Biotechnology. Federal University of Uberlândia. Uberlândia, Brazil.Department of Molecular Medicine. University of Rome La Sapienza. Rome, Italy.Laboratory of Molecular and Cellular Genetics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.Laboratory of Molecular and Cellular Genetics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.Laboratory of Genetics. Institute of Biotechnology. Federal University of Uberlândia. Uberlândia, MG, Brazil.Faculty of Computer Science. Federal University of Uberlândia. Uberlândia, Brazil.René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Laboratory of Genetics. Institute of Biotechnology. Federal University of Uberlândia. Uberlândia, MG, Brazil.Laboratory of Genetics. Institute of Biotechnology. Federal University of Uberlândia. Uberlândia, Brazil.Stingless bees are a diverse group with a relevant role in pollinating native species. Its diet is rich in carbohydrates and proteins, by collecting pollen and nectar supplies the development of its offspring. Fermentation of these products is associated with microorganisms in the colony. However, the composition of microorganisms that comprise this microbiome and its fundamental role in colony development is still unclear. To characterize the colonizing microorganisms of larval food in the brood cells of stingless bees Frieseomelitta varia, Melipona quadrifasciata, Melipona scutellaris, and Tetragonisca angustula, we have utilized molecular and culture-based techniques. Bacteria of the phyla Firmicutes, Proteobacteria, Actinobacteria, and fungi of the phyla Ascomycota, Basidiomycota, Mucoromycota, and Mortierellomycota were found. Diversity analysis showed that F. varia had a greater diversity of bacteria in its microbiota, and T. angustula had a greater diversity of fungi. The isolation technique allowed the identification of 189 bacteria and 75 fungi. In summary, this research showed bacteria and fungi associated with the species F. varia, M. quadrifasciata, M. scutellaris, and T. angustula, which may play an essential role in the survival of these organisms. Besides that, a biobank with bacteria and fungus isolates from LF of Brazilian stingless bees was created, which can be used for different studies and the prospection of biotechnology compounds.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBacteria, yeasts, and fungi associated with larval.pdfBacteria, yeasts, and fungi associated with larval.pdfapplication/pdf6489541https://www.arca.fiocruz.br/bitstream/icict/57945/2/Bacteria%2c%20yeasts%2c%20and%20fungi%20associated%20with%20larval.pdf6df281ea34efa135d6d9b16409ccb74fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57945/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/579452023-04-24 14:54:54.751oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-04-24T17:54:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
title Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
spellingShingle Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
Santos, Ana Carolina Costa
title_short Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
title_full Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
title_fullStr Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
title_full_unstemmed Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
title_sort Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees
author Santos, Ana Carolina Costa
author_facet Santos, Ana Carolina Costa
Borges, Luiza Diniz Ferreira
Rocha, Nina Dias Coelho
Azevedo, Vasco Ariston de Carvalho
Bonetti, Ana Maria
Santos, Anderson Rodrigues Dos
Fernandes, Gabriel da Rocha
Dantas, Raquel Cristina Cavalcanti
Ueira-Vieira, Carlos
author_role author
author2 Borges, Luiza Diniz Ferreira
Rocha, Nina Dias Coelho
Azevedo, Vasco Ariston de Carvalho
Bonetti, Ana Maria
Santos, Anderson Rodrigues Dos
Fernandes, Gabriel da Rocha
Dantas, Raquel Cristina Cavalcanti
Ueira-Vieira, Carlos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Ana Carolina Costa
Borges, Luiza Diniz Ferreira
Rocha, Nina Dias Coelho
Azevedo, Vasco Ariston de Carvalho
Bonetti, Ana Maria
Santos, Anderson Rodrigues Dos
Fernandes, Gabriel da Rocha
Dantas, Raquel Cristina Cavalcanti
Ueira-Vieira, Carlos
description 2030-12-31
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-24T17:54:54Z
dc.date.available.fl_str_mv 2023-04-24T17:54:54Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Ana Carolina Costa et al. Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees. Sci Rep, v. 13, n. 1, 5147, 2023. doi: 10.1038/s41598-023-32298-w.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57945
dc.identifier.issn.en_US.fl_str_mv 2045-2322
identifier_str_mv SANTOS, Ana Carolina Costa et al. Bacteria, yeasts, and fungi associated with larval food of Brazilian native stingless bees. Sci Rep, v. 13, n. 1, 5147, 2023. doi: 10.1038/s41598-023-32298-w.
2045-2322
url https://www.arca.fiocruz.br/handle/icict/57945
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57945/2/Bacteria%2c%20yeasts%2c%20and%20fungi%20associated%20with%20larval.pdf
https://www.arca.fiocruz.br/bitstream/icict/57945/1/license.txt
bitstream.checksum.fl_str_mv 6df281ea34efa135d6d9b16409ccb74f
5a560609d32a3863062d77ff32785d58
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008980851556352

Registros relacionados