Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27873 |
Resumo: | Este projeto contou com o apoio de doações do Conselho Nacional de Pesquisa (CNPq 475647 / 2004-1 e 471119 / 2006-2) e da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). |
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Melo, Janaina Viana deJones, Gareth WynBerry, ColinVasconcelos, Romero Henrique TeixeiraOliveira, Cláudia Maria Fontes deFurtado, André FreirePeixoto, Christina AlvesSilva-Filha, Maria Helena Neves Lobo2018-08-06T13:09:13Z2018-08-06T13:09:13Z2009DE MELO, Janaina Viana et al. Cytopathological Effects of Bacillus Sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex Quinquefasciatus Larvae. Applied and Environmental Microbiology, v. 75, n. 14, p. 4782–4789, jul. 2009.1098-5336https://www.arca.fiocruz.br/handle/icict/2787310.1128/AEM.00811-09Este projeto contou com o apoio de doações do Conselho Nacional de Pesquisa (CNPq 475647 / 2004-1 e 471119 / 2006-2) e da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Cardiff University. Cardiff School of Biosciences. Museum Avenue, Cardiff, United Kingdom.Cardiff University. Cardiff School of Biosciences. Museum Avenue, Cardiff, United Kingdom.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.The Cry48Aa/Cry49Aa mosquitocidal two-component toxin was recently characterized from Bacillus sphaericus strain IAB59 and is uniquely composed of a three-domain Cry protein toxin (Cry48Aa) and a binary (Bin) toxin-like protein (Cry49Aa). Its mode of action has not been elucidated, but a remarkable feature of this protein is the high toxicity against species from the Culex complex, besides its capacity to overcome Culex resistance to the Bin toxin, the major insecticidal factor in B. sphaericus-based larvicides. The goal of this work was to investigate the ultrastructural effects of Cry48Aa/Cry49Aa on midgut cells of Bin-toxin-susceptible and -resistant Culex quinquefasciatus larvae. The major cytopathological effects observed after Cry48Aa/Cry49Aa treatment were intense mitochondrial vacuolation, breakdown of endoplasmic reticulum, production of cytoplasmic vacuoles, and microvillus disruption. These effects were similar in Bin-toxin-susceptible and -resistant larvae and demonstrated that Cry48Aa/Cry49Aa toxin interacts with and displays toxic effects on cells lacking receptors for the Bin toxin, while B. sphaericus IAB59-resistant larvae did not show mortality after treatment with Cry48Aa/Cry49Aa toxin. The cytopathological alterations in Bin-toxin-resistant larvae provoked by Cry48Aa/Cry49Aa treatment were similar to those observed when larvae were exposed to a synergistic mixture of Bin/Cry11Aa toxins. Such effects seemed to result from a combined action of Cry-like and Bin-like toxins. The complex effects caused by Cry48Aa/Cry49Aa provide evidence for the potential of these toxins as active ingredients of a new generation of biolarvicides that conjugate insecticidal factors with distinct sites of action, in order to manage mosquito resistance.engCytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimaisBacilo / metabolismoToxinas bacterianas / farmacologiaCulex / efeitos de drogasCulex / ultraestruturaTrato Gastrointestinal / efeitos de drogasTrato Gastrointestinal / ultraestruturaInseticidas / farmacologiaLarva / efeitos de drogasLarva / ultraestruturaOrganelas / efeitos de drogasOrganelas / ultraestruturaAnálise de Sobrevivênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/27873/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALCytopathological Effects of Bacillus sphaericus.pdfCytopathological Effects of Bacillus sphaericus.pdfapplication/pdf2761584https://www.arca.fiocruz.br/bitstream/icict/27873/2/Cytopathological%20Effects%20of%20Bacillus%20sphaericus.pdf6faa3ac452f3798ab7797d4eeba4f2dfMD52TEXTCytopathological Effects of Bacillus sphaericus.pdf.txtCytopathological Effects of Bacillus sphaericus.pdf.txtExtracted texttext/plain33241https://www.arca.fiocruz.br/bitstream/icict/27873/3/Cytopathological%20Effects%20of%20Bacillus%20sphaericus.pdf.txt4f3bdab1a889f3ac073ad89d85918e1cMD53icict/278732018-08-15 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| dc.title.pt_BR.fl_str_mv |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| title |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| spellingShingle |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae Melo, Janaina Viana de Animais Bacilo / metabolismo Toxinas bacterianas / farmacologia Culex / efeitos de drogas Culex / ultraestrutura Trato Gastrointestinal / efeitos de drogas Trato Gastrointestinal / ultraestrutura Inseticidas / farmacologia Larva / efeitos de drogas Larva / ultraestrutura Organelas / efeitos de drogas Organelas / ultraestrutura Análise de Sobrevivência |
| title_short |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| title_full |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| title_fullStr |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| title_full_unstemmed |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| title_sort |
Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae |
| author |
Melo, Janaina Viana de |
| author_facet |
Melo, Janaina Viana de Jones, Gareth Wyn Berry, Colin Vasconcelos, Romero Henrique Teixeira Oliveira, Cláudia Maria Fontes de Furtado, André Freire Peixoto, Christina Alves Silva-Filha, Maria Helena Neves Lobo |
| author_role |
author |
| author2 |
Jones, Gareth Wyn Berry, Colin Vasconcelos, Romero Henrique Teixeira Oliveira, Cláudia Maria Fontes de Furtado, André Freire Peixoto, Christina Alves Silva-Filha, Maria Helena Neves Lobo |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Melo, Janaina Viana de Jones, Gareth Wyn Berry, Colin Vasconcelos, Romero Henrique Teixeira Oliveira, Cláudia Maria Fontes de Furtado, André Freire Peixoto, Christina Alves Silva-Filha, Maria Helena Neves Lobo |
| dc.subject.decs.pt_BR.fl_str_mv |
Animais Bacilo / metabolismo Toxinas bacterianas / farmacologia Culex / efeitos de drogas Culex / ultraestrutura Trato Gastrointestinal / efeitos de drogas Trato Gastrointestinal / ultraestrutura Inseticidas / farmacologia Larva / efeitos de drogas Larva / ultraestrutura Organelas / efeitos de drogas Organelas / ultraestrutura Análise de Sobrevivência |
| topic |
Animais Bacilo / metabolismo Toxinas bacterianas / farmacologia Culex / efeitos de drogas Culex / ultraestrutura Trato Gastrointestinal / efeitos de drogas Trato Gastrointestinal / ultraestrutura Inseticidas / farmacologia Larva / efeitos de drogas Larva / ultraestrutura Organelas / efeitos de drogas Organelas / ultraestrutura Análise de Sobrevivência |
| description |
Este projeto contou com o apoio de doações do Conselho Nacional de Pesquisa (CNPq 475647 / 2004-1 e 471119 / 2006-2) e da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). |
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2009 |
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2009 |
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2018-08-06T13:09:13Z |
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2018-08-06T13:09:13Z |
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info:eu-repo/semantics/article |
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DE MELO, Janaina Viana et al. Cytopathological Effects of Bacillus Sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex Quinquefasciatus Larvae. Applied and Environmental Microbiology, v. 75, n. 14, p. 4782–4789, jul. 2009. |
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https://www.arca.fiocruz.br/handle/icict/27873 |
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1098-5336 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/AEM.00811-09 |
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DE MELO, Janaina Viana et al. Cytopathological Effects of Bacillus Sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex Quinquefasciatus Larvae. Applied and Environmental Microbiology, v. 75, n. 14, p. 4782–4789, jul. 2009. 1098-5336 10.1128/AEM.00811-09 |
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