Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control

Detalhes bibliográficos
Autor(a) principal: Caetano, Diogo Gama
Data de Publicação: 2018
Outros Autores: Côrtes, Fernanda Heloise, Bello, Gonzalo, Teixeira, Sylvia Lopes Maia, Hoagland, Brenda Regina de Siqueira, Grinsztejn, Beatriz, Santos, Valdiléa Gonçalves Veloso dos, Guimarães, Monick Lindenmeyer, Morgado, Mariza Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30402
Resumo: Acknowledgements: We thank the patients, nurses, and clinicians who participated in the study. We also thank Egydio Sampaio for support in the recruitment of patients and all INI staff from the blood collection sector. Finally, we are thankful for the CD4+ T cell count and HIV-1 viral load clinical services from the Brazilian Ministry of Health National Network, and the FIOCRUZ PDTIS NGS Platform (RPT01J).
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spelling Caetano, Diogo GamaCôrtes, Fernanda HeloiseBello, GonzaloTeixeira, Sylvia Lopes MaiaHoagland, Brenda Regina de SiqueiraGrinsztejn, BeatrizSantos, Valdiléa Gonçalves Veloso dosGuimarães, Monick LindenmeyerMorgado, Mariza Gonçalves2018-12-05T16:21:55Z2018-12-05T16:21:55Z2018CAETANO, D. G. et al. Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control. Retrovirology, v. 15, n. 1, p. 62, 2018.1742-4690https://www.arca.fiocruz.br/handle/icict/3040210.1186/s12977-018-0444-zAcknowledgements: We thank the patients, nurses, and clinicians who participated in the study. We also thank Egydio Sampaio for support in the recruitment of patients and all INI staff from the blood collection sector. Finally, we are thankful for the CD4+ T cell count and HIV-1 viral load clinical services from the Brazilian Ministry of Health National Network, and the FIOCRUZ PDTIS NGS Platform (RPT01J).2019-10-30Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Aids e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Despite the low level of viral replication in HIV controllers (HICs), studies have reported viral mutations related to escape from cytotoxic T-lymphocyte (CTL) response in HIV-1 plasma sequences. Thus, evaluating the dynamics of the emergence of CTL-escape mutants in HICs reservoirs is important for understanding viremia control. To analyze the HIV-1 mutational profile and dynamics of CTL-escape mutants in HICs, we selected 11 long-term non-progressor individuals and divided them into the following groups: (1) viremic controllers (VCs; n = 5) and (2) elite controllers (ECs; n = 6). For each individual, we used HIV-1 proviral DNA from PBMCs related to earliest (VE) and latest (VL) visits to obtain gag and nef sequences using the Illumina HiSeq system. The consensus of each mapped gene was used to assess viral divergence, and next-generation sequencing data were employed to identify SNPs and variations within and flanking CTL epitopes.engBMCNext-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia controlinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCTL epitopeEscape mutantHIV controllerHIV-1Next-generation sequencingSingle-nucleotide polymorphisminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/30402/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALNext-generation sequencing_Valdilea_Veloso_INI_Lapclin-AIDS_2018.pdfNext-generation 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dc.title.pt_BR.fl_str_mv Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
title Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
spellingShingle Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
Caetano, Diogo Gama
CTL epitope
Escape mutant
HIV controller
HIV-1
Next-generation sequencing
Single-nucleotide polymorphism
title_short Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
title_full Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
title_fullStr Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
title_full_unstemmed Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
title_sort Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control
author Caetano, Diogo Gama
author_facet Caetano, Diogo Gama
Côrtes, Fernanda Heloise
Bello, Gonzalo
Teixeira, Sylvia Lopes Maia
Hoagland, Brenda Regina de Siqueira
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso dos
Guimarães, Monick Lindenmeyer
Morgado, Mariza Gonçalves
author_role author
author2 Côrtes, Fernanda Heloise
Bello, Gonzalo
Teixeira, Sylvia Lopes Maia
Hoagland, Brenda Regina de Siqueira
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso dos
Guimarães, Monick Lindenmeyer
Morgado, Mariza Gonçalves
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Caetano, Diogo Gama
Côrtes, Fernanda Heloise
Bello, Gonzalo
Teixeira, Sylvia Lopes Maia
Hoagland, Brenda Regina de Siqueira
Grinsztejn, Beatriz
Santos, Valdiléa Gonçalves Veloso dos
Guimarães, Monick Lindenmeyer
Morgado, Mariza Gonçalves
dc.subject.en.pt_BR.fl_str_mv CTL epitope
Escape mutant
HIV controller
HIV-1
Next-generation sequencing
Single-nucleotide polymorphism
topic CTL epitope
Escape mutant
HIV controller
HIV-1
Next-generation sequencing
Single-nucleotide polymorphism
description Acknowledgements: We thank the patients, nurses, and clinicians who participated in the study. We also thank Egydio Sampaio for support in the recruitment of patients and all INI staff from the blood collection sector. Finally, we are thankful for the CD4+ T cell count and HIV-1 viral load clinical services from the Brazilian Ministry of Health National Network, and the FIOCRUZ PDTIS NGS Platform (RPT01J).
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-05T16:21:55Z
dc.date.available.fl_str_mv 2018-12-05T16:21:55Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAETANO, D. G. et al. Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control. Retrovirology, v. 15, n. 1, p. 62, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30402
dc.identifier.issn.pt_BR.fl_str_mv 1742-4690
dc.identifier.doi.none.fl_str_mv 10.1186/s12977-018-0444-z
identifier_str_mv CAETANO, D. G. et al. Next-generation sequencing analyses of the emergence and maintenance of mutations in CTL epitopes in HIV controllers with differential viremia control. Retrovirology, v. 15, n. 1, p. 62, 2018.
1742-4690
10.1186/s12977-018-0444-z
url https://www.arca.fiocruz.br/handle/icict/30402
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30402/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/30402/2/Next-generation%20sequencing_Valdilea_Veloso_INI_Lapclin-AIDS_2018.pdf
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