Functional activity of monocytes and macrophages in HTLV-1 infected subjects
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24936 |
Resumo: | CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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Amorim, Camila FariasSouza, Anselmo de SantanaDiniz, Angela GiudiceCarvalho, Natália BarbosaSantos, Silvane Maria BragaCarvalho Filho, Edgar Marcelino2018-02-26T13:45:01Z2018-02-26T13:45:01Z2014AMORIM, C. F. et al. Functional activity of monocytes and macrophages in HTLV-1 infected subjects. PLoS Neglected Tropical Diseases, v. 8, n. 12, p. e3399, 2014.1935-2727https://www.arca.fiocruz.br/handle/icict/2493610.1371/journal.pntd.0003399CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institute of Health (NIH) grant AI079238 and by the Instituto Nacional de Ciências e Tecnologia de Doenças Tropicais (INCT-DT, CNPq)Universidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Departamento de Ciências Biológicas. Feira de Santana, Bahia, Brazil / Instituto Nacional de Ciências e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilThe Human T lymphotropic virus type-1 (HTLV-1) infects predominantly T cells, inducing proliferation and lymphocyte activation. Additionally, HTLV-1 infected subjects are more susceptible to other infections caused by other intracellular agents. Monocytes/macrophages are important cells in the defense against intracellular pathogens. Our aims were to determine the frequency of monocytes subsets, expression of co-stimulatory molecules in these cells and to evaluate microbicidal ability and cytokine and chemokine production by macrophages from HTLV-1 infected subjects. Participants were 23 HTLV-1 carriers (HC), 22 HAM/TSP patients and 22 healthy subjects (HS) not infected with HTLV-1. The frequencies of monocyte subsets and expression of co-stimulatory molecules were determined by flow cytometry. Macrophages were infected with L. braziliensis or stimulated with LPS. Microbicidal activity of macrophages was determined by optic microscopy. Cytokines/chemokines from macrophage supernatants were measured by ELISA. HAM/TSP patients showed an increase frequency of intermediate monocytes, but expression of co-stimulatory molecules was similar between the groups. Macrophages from HTLV-1 infected individuals were infected with L. braziliensis at the same ratio than macrophages from HS, and all the groups had the same ability to kill Leishmania parasites. However, macrophages from HTLV-1 infected subjects produced more CXCL9 and CCL5, and less IL-10 than cells from HS. While there was no correlation between IFN-γ and cytokine/chemokine production by macrophages, there was a correlation between proviral load and TNF and CXCL10. These data showed a dissociation between the inflammatory response and microbicidal ability of macrophages from HTLV-1 infected subjects. While macrophages ability to kill an intracellular pathogen did not differ among HTLV-1 infected subjects, these cells secreted high amount of chemokines even in unstimulated cultures. Moreover the increasing inflammatory activity of macrophages was similar in HAM/TSP patients and HC and it was related to HTLV-1 proviral load rather than the high IFN-γ production observed in these subjects.engPublic Library of ScienceHTLV-1MonócitosMacrófagosHumanosAdultoInfecçãoHTLV-1MonocytesMacrophagesHumansAdultInfectionFunctional activity of monocytes and macrophages in HTLV-1 infected subjectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24936/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAmorim CF Functional Activity of Monocytes and Macrophages....pdfAmorim CF Functional Activity of Monocytes and 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| dc.title.pt_BR.fl_str_mv |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| title |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| spellingShingle |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects Amorim, Camila Farias HTLV-1 Monócitos Macrófagos Humanos Adulto Infecção HTLV-1 Monocytes Macrophages Humans Adult Infection |
| title_short |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| title_full |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| title_fullStr |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| title_full_unstemmed |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| title_sort |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects |
| author |
Amorim, Camila Farias |
| author_facet |
Amorim, Camila Farias Souza, Anselmo de Santana Diniz, Angela Giudice Carvalho, Natália Barbosa Santos, Silvane Maria Braga Carvalho Filho, Edgar Marcelino |
| author_role |
author |
| author2 |
Souza, Anselmo de Santana Diniz, Angela Giudice Carvalho, Natália Barbosa Santos, Silvane Maria Braga Carvalho Filho, Edgar Marcelino |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Amorim, Camila Farias Souza, Anselmo de Santana Diniz, Angela Giudice Carvalho, Natália Barbosa Santos, Silvane Maria Braga Carvalho Filho, Edgar Marcelino |
| dc.subject.other.pt_BR.fl_str_mv |
HTLV-1 Monócitos Macrófagos Humanos Adulto Infecção |
| topic |
HTLV-1 Monócitos Macrófagos Humanos Adulto Infecção HTLV-1 Monocytes Macrophages Humans Adult Infection |
| dc.subject.en.pt_BR.fl_str_mv |
HTLV-1 Monocytes Macrophages Humans Adult Infection |
| description |
CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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2014 |
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2014 |
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2018-02-26T13:45:01Z |
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2018-02-26T13:45:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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AMORIM, C. F. et al. Functional activity of monocytes and macrophages in HTLV-1 infected subjects. PLoS Neglected Tropical Diseases, v. 8, n. 12, p. e3399, 2014. |
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https://www.arca.fiocruz.br/handle/icict/24936 |
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1935-2727 |
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10.1371/journal.pntd.0003399 |
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AMORIM, C. F. et al. Functional activity of monocytes and macrophages in HTLV-1 infected subjects. PLoS Neglected Tropical Diseases, v. 8, n. 12, p. e3399, 2014. 1935-2727 10.1371/journal.pntd.0003399 |
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