Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues

Detalhes bibliográficos
Autor(a) principal: Santos, Danielle Silva dos
Data de Publicação: 2017
Outros Autores: Albuquerque, Juliana Barreto de, Guerra, Bárbara, Moreira, Otacilio C., Berbert, Luiz Ricardo, Ramos, Mariana Tavares, Mascarenhas, Barbara Angelica S., Britto, Constança, Morrot, Alexandre, Villa-Verde, Déa M. Serra, Garzoni, Luciana Ribeiro, Savino, Wilson, Almeida, Vinicius Cotta de, Meis, Juliana de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20153
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
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spelling Santos, Danielle Silva dosAlbuquerque, Juliana Barreto deGuerra, BárbaraMoreira, Otacilio C.Berbert, Luiz RicardoRamos, Mariana TavaresMascarenhas, Barbara Angelica S.Britto, ConstançaMorrot, AlexandreVilla-Verde, Déa M. SerraGarzoni, Luciana RibeiroSavino, WilsonAlmeida, Vinicius Cotta deMeis, Juliana de2017-07-20T11:38:43Z2017-07-20T11:38:43Z2017SANTOS, Danielle Silva dos; et al. Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. PLoS Negl Trop Dis., v.11, n.4, e0005507, 26p, Apr. 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2015310.1371/journal. pntd.00055071935-2735engPublic Library of ScienceTrypanosoma cruziDoença de ChagasTransmissão oralTrypanosoma cruziChagas diseaseOral transmissionUnraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissuesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the most important route of infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed the importance of parasite contact with oral cavity to induce a highly severe acute disease in mice. However, it remains uncertain the primary site of parasite entry and multiplication due to an oral infection. Here, we evaluated the presence of T. cruzi Dm28c luciferase (Dm28c-luc) parasites in orally infected mice, by bioluminescence and quantitative real-time PCR. In vivo bioluminescent images indicated the nasomaxillary region as the site of parasite invasion in the host, becoming consistently infected throughout the acute phase. At later moments, 7 and 21 days post-infection (dpi), luminescent signal is denser in the thorax, abdomen and genital region, because of parasite dissemination in different tissues. Ex vivo analysis demonstrated that the nasomaxillary region, heart, mandibular lymph nodes, liver, spleen, brain, epididymal fat associated to male sex organs, salivary glands, cheek muscle, mesenteric fat and lymph nodes, stomach, esophagus, small and large intestine are target tissues at latter moments of infection. In the same line, amastigote nests of Dm28c GFP T. cruzi were detected in the nasal cavity of 6 dpi mice. Parasite quantification by real-time qPCR at 7 and 21 dpi showed predominant T. cruzi detection and expansion in mouse nasal cavity. Moreover, T. cruzi DNA was also observed in the mandibular lymph nodes, pituitary gland, heart, liver, small intestine and spleen at 7 dpi, and further, disseminated to other tissues, such as the brain, stomach, esophagus and large intestine at 21 dpi. Our results clearly demonstrated that oral cavity and adjacent compartments is the main target region in oral T. cruzi infection leading to parasite multiplication at the nasal cavity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20153/1/license.txt5a560609d32a3863062d77ff32785d58MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20153/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdea_Villaverde_etal_IOC_2017.pdfdea_Villaverde_etal_IOC_2017.pdfapplication/pdf4095153https://www.arca.fiocruz.br/bitstream/icict/20153/2/dea_Villaverde_etal_IOC_2017.pdf9d778615a28eca5e7d9aecc418adb476MD52TEXTdea_Villaverde_etal_IOC_2017.pdf.txtdea_Villaverde_etal_IOC_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
title Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
spellingShingle Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
Santos, Danielle Silva dos
Trypanosoma cruzi
Doença de Chagas
Transmissão oral
Trypanosoma cruzi
Chagas disease
Oral transmission
title_short Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
title_full Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
title_fullStr Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
title_full_unstemmed Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
title_sort Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
author Santos, Danielle Silva dos
author_facet Santos, Danielle Silva dos
Albuquerque, Juliana Barreto de
Guerra, Bárbara
Moreira, Otacilio C.
Berbert, Luiz Ricardo
Ramos, Mariana Tavares
Mascarenhas, Barbara Angelica S.
Britto, Constança
Morrot, Alexandre
Villa-Verde, Déa M. Serra
Garzoni, Luciana Ribeiro
Savino, Wilson
Almeida, Vinicius Cotta de
Meis, Juliana de
author_role author
author2 Albuquerque, Juliana Barreto de
Guerra, Bárbara
Moreira, Otacilio C.
Berbert, Luiz Ricardo
Ramos, Mariana Tavares
Mascarenhas, Barbara Angelica S.
Britto, Constança
Morrot, Alexandre
Villa-Verde, Déa M. Serra
Garzoni, Luciana Ribeiro
Savino, Wilson
Almeida, Vinicius Cotta de
Meis, Juliana de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Danielle Silva dos
Albuquerque, Juliana Barreto de
Guerra, Bárbara
Moreira, Otacilio C.
Berbert, Luiz Ricardo
Ramos, Mariana Tavares
Mascarenhas, Barbara Angelica S.
Britto, Constança
Morrot, Alexandre
Villa-Verde, Déa M. Serra
Garzoni, Luciana Ribeiro
Savino, Wilson
Almeida, Vinicius Cotta de
Meis, Juliana de
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Transmissão oral
topic Trypanosoma cruzi
Doença de Chagas
Transmissão oral
Trypanosoma cruzi
Chagas disease
Oral transmission
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas disease
Oral transmission
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-20T11:38:43Z
dc.date.available.fl_str_mv 2017-07-20T11:38:43Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Danielle Silva dos; et al. Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. PLoS Negl Trop Dis., v.11, n.4, e0005507, 26p, Apr. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20153
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal. pntd.0005507
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv SANTOS, Danielle Silva dos; et al. Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues. PLoS Negl Trop Dis., v.11, n.4, e0005507, 26p, Apr. 2017.
1935-2727
10.1371/journal. pntd.0005507
1935-2735
url https://www.arca.fiocruz.br/handle/icict/20153
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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