Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.

Detalhes bibliográficos
Autor(a) principal: Medeiros, Fernanda Alvarenga Cardoso
Data de Publicação: 2017
Outros Autores: Gomes, Luciana Inácia, Oliveira, Edward José de, Souza, Carolina Senra Alves de, Mourão, Maria Vitória, Cota, Gláucia Fernandes, Marques, Letícia Helena dos Santos, Carneiro, Mariângela, Rabello, Ana Lúcia Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1155/2017/7364854
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26779
Resumo: 2020-01-01
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spelling Medeiros, Fernanda Alvarenga CardosoGomes, Luciana InáciaOliveira, Edward José deSouza, Carolina Senra Alves deMourão, Maria VitóriaCota, Gláucia FernandesMarques, Letícia Helena dos SantosCarneiro, MariângelaRabello, Ana Lúcia Teles2018-06-07T17:29:58Z2018-06-07T17:29:58Z2017MEDEIROS, Fernanda Alvarenga Cardoso et al. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. J Trop Med., v. 2017, 7364854, 20171687-9686https://www.arca.fiocruz.br/handle/icict/2677910.1155/2017/7364854engHindawi Publishing CorporationLeishmaniose visceral/diagnosticokDNA PCR-ELISAvisceral leishmaniasis/diagnostickDNA PCR-ELISADevelopment and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrazilA kDNA PCR enzyme-linked immunosorbent assay (kDNA PCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed. The detection limit of the reaction, precision measurements, and cut-off of the kDNA PCR-ELISA were defined in a proof-of-concept phase. A reference strain of Leishmania (Leishmania) infantum and a bank of 14 peripheral blood samples from immunocompetent patients with VL were characterized using techniques considered gold standards, and 11 blood samples obtained from healthy individuals of an endemic area were also assessed. Phase II evaluation determined the performance of the assay in peripheral blood samples from 105 patients with VL (adults and children), 25 patients with Leishmania/HIV coinfection, 40 healthy individuals, and 33 asymptomatic individuals living in endemic areas. The kDNA PCR-ELISA exhibited satisfactory precision, with a detection limit of 0.07 fg of DNA from L. (L.) infantum and 1 parasite/mL blood. The overall sensitivity of the assay for all groups studied was 100% (95% confidence interval [CI]: 97.1–100%), and the specificity was 95% (95% CI: 83.5–98.6%). The kDNA PCR-ELISA was shown to be a useful tool for VL symptomatic and asymptomatic individuals diagnosis and its use in endemic countries may help monitor control interventions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/26779/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDevelopment and Validation of a PCR-ELISA.pdfDevelopment and Validation of a PCR-ELISA.pdfapplication/pdf888593https://www.arca.fiocruz.br/bitstream/icict/26779/2/Development%20and%20Validation%20of%20a%20PCR-ELISA.pdf004f8bfe79bb455fd2288fd1dc90cf5fMD52TEXTDevelopment and Validation of a PCR-ELISA.pdf.txtDevelopment and Validation of a PCR-ELISA.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
title Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
spellingShingle Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
Medeiros, Fernanda Alvarenga Cardoso
Leishmaniose visceral/diagnostico
kDNA PCR-ELISA
visceral leishmaniasis/diagnostic
kDNA PCR-ELISA
title_short Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
title_full Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
title_fullStr Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
title_full_unstemmed Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
title_sort Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.
author Medeiros, Fernanda Alvarenga Cardoso
author_facet Medeiros, Fernanda Alvarenga Cardoso
Gomes, Luciana Inácia
Oliveira, Edward José de
Souza, Carolina Senra Alves de
Mourão, Maria Vitória
Cota, Gláucia Fernandes
Marques, Letícia Helena dos Santos
Carneiro, Mariângela
Rabello, Ana Lúcia Teles
author_role author
author2 Gomes, Luciana Inácia
Oliveira, Edward José de
Souza, Carolina Senra Alves de
Mourão, Maria Vitória
Cota, Gláucia Fernandes
Marques, Letícia Helena dos Santos
Carneiro, Mariângela
Rabello, Ana Lúcia Teles
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Medeiros, Fernanda Alvarenga Cardoso
Gomes, Luciana Inácia
Oliveira, Edward José de
Souza, Carolina Senra Alves de
Mourão, Maria Vitória
Cota, Gláucia Fernandes
Marques, Letícia Helena dos Santos
Carneiro, Mariângela
Rabello, Ana Lúcia Teles
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral/diagnostico
kDNA PCR-ELISA
topic Leishmaniose visceral/diagnostico
kDNA PCR-ELISA
visceral leishmaniasis/diagnostic
kDNA PCR-ELISA
dc.subject.en.pt_BR.fl_str_mv visceral leishmaniasis/diagnostic
kDNA PCR-ELISA
description 2020-01-01
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-06-07T17:29:58Z
dc.date.available.fl_str_mv 2018-06-07T17:29:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDEIROS, Fernanda Alvarenga Cardoso et al. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. J Trop Med., v. 2017, 7364854, 2017
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26779
dc.identifier.issn.pt_BR.fl_str_mv 1687-9686
dc.identifier.doi.none.fl_str_mv 10.1155/2017/7364854
identifier_str_mv MEDEIROS, Fernanda Alvarenga Cardoso et al. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. J Trop Med., v. 2017, 7364854, 2017
1687-9686
10.1155/2017/7364854
url https://www.arca.fiocruz.br/handle/icict/26779
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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