Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Schmidt, Juliana C.
Data de Publicação: 2011
Outros Autores: Soares, Maurilio José, Goldenberg, Samuel, Pavoni, Daniela Parada, Krieger, Marco Aurélio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40112
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Comunitária da Região de Chapecó. Chapecó, SC, Brasil.
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spelling Schmidt, Juliana C.Soares, Maurilio JoséGoldenberg, SamuelPavoni, Daniela ParadaKrieger, Marco Aurélio2020-02-21T15:25:43Z2020-02-21T15:25:43Z2011SCHMIDT, Juliana C. et al. Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 1, p. 70-77, 2011.1678-8060https://www.arca.fiocruz.br/handle/icict/4011210.1590/s0074-027620110001000121678-8060porFundação Oswaldo Cruz. Instituto Oswaldo CruzTrypanosoma cruziHSP70 Heat-Shock ProteinsStress, PhysiologicalProteínas HSP70 de Choque TérmicoEstrés FisiológicoProteínas de Choque Térmico HSP70Estresse FisiológicoCharacterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Comunitária da Região de Chapecó. Chapecó, SC, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.The life cycle of the protozoan Trypanosoma cruzi exposes it to several environmental stresses in its invertebrate and vertebrate hosts. Stress conditions are involved in parasite differentiation, but little is known about the stress response proteins involved. We report here the first characterization of stress-induced protein-1 (STI-1) in T. cruzi (TcSTI-1). This co-chaperone is produced in response to stress and mediates the formation of a complex between the stress proteins HSP70 and HSP90 in other organisms. Despite the similarity of TcSTI-1 to STI-1 proteins in other organisms, its expression profile in response to various stress conditions, such as heat shock, acidic pH or nutrient starvation, is quite different. Neither polysomal mRNA nor protein levels changed in exponentially growing epimastigotes cultured under any of the stress conditions studied. Increased levels of TcSTI-1 were observed in epimastigotes subjected to nutritional stress in the late growth phase. Co-immunoprecipitation assays revealed an association between TcSTI-1 and TcHSP70 in T. cruzi epimastigotes. Immunolocalization demonstrated that TcSTI-1 was distributed throughout the cytoplasm and there was some colocalization of TcSTI-1 and TcHSP70 around the nucleus. Thus, TcSTI-1 associates with TcHSP70 and TcSTI-1 expression is induced when the parasites are subjected to stress conditions during specific growth phase.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40112/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL12ok.pdf12ok.pdfapplication/pdf1493970https://www.arca.fiocruz.br/bitstream/icict/40112/2/12ok.pdff18de62b090b3739f8983f3fac389363MD52TEXT12ok.pdf.txt12ok.pdf.txtExtracted texttext/plain41008https://www.arca.fiocruz.br/bitstream/icict/40112/3/12ok.pdf.txtffba5b89bd044ff2e0e613731d2a3880MD53icict/401122020-02-25 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dc.title.pt_BR.fl_str_mv Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
title Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
spellingShingle Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
Schmidt, Juliana C.
Trypanosoma cruzi
HSP70 Heat-Shock Proteins
Stress, Physiological
Proteínas HSP70 de Choque Térmico
Estrés Fisiológico
Proteínas de Choque Térmico HSP70
Estresse Fisiológico
title_short Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
title_full Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
title_fullStr Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
title_full_unstemmed Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
title_sort Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
author Schmidt, Juliana C.
author_facet Schmidt, Juliana C.
Soares, Maurilio José
Goldenberg, Samuel
Pavoni, Daniela Parada
Krieger, Marco Aurélio
author_role author
author2 Soares, Maurilio José
Goldenberg, Samuel
Pavoni, Daniela Parada
Krieger, Marco Aurélio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Schmidt, Juliana C.
Soares, Maurilio José
Goldenberg, Samuel
Pavoni, Daniela Parada
Krieger, Marco Aurélio
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
HSP70 Heat-Shock Proteins
Stress, Physiological
Proteínas HSP70 de Choque Térmico
Estrés Fisiológico
Proteínas de Choque Térmico HSP70
Estresse Fisiológico
dc.subject.en.pt_BR.fl_str_mv HSP70 Heat-Shock Proteins
Stress, Physiological
dc.subject.es.pt_BR.fl_str_mv Proteínas HSP70 de Choque Térmico
Estrés Fisiológico
dc.subject.decs.pt_BR.fl_str_mv Proteínas de Choque Térmico HSP70
Estresse Fisiológico
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Comunitária da Região de Chapecó. Chapecó, SC, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-02-21T15:25:43Z
dc.date.available.fl_str_mv 2020-02-21T15:25:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHMIDT, Juliana C. et al. Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 1, p. 70-77, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40112
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762011000100012
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SCHMIDT, Juliana C. et al. Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 1, p. 70-77, 2011.
1678-8060
10.1590/s0074-02762011000100012
url https://www.arca.fiocruz.br/handle/icict/40112
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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