Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Souza, Renata Torres de
Data de Publicação: 2011
Outros Autores: Lima, Fabio Mitsuo, Barros, Roberto Rudge de Moraes, Cortez, Danielle Rodrigues, Santos, Michele F., Veas, Esteban Mauricio Cordero, Ruiz, Jerônimo Conceição, Goldenberg, Samuel, Teixeira, Marta Maria Geraldes, Silveira, José Franco da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7922
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil
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spelling Souza, Renata Torres de,Lima, Fabio MitsuoBarros, Roberto Rudge de MoraesCortez, Danielle RodriguesSantos, Michele F.Veas, Esteban Mauricio CorderoRuiz, Jerônimo ConceiçãoGoldenberg, SamuelTeixeira, Marta Maria GeraldesSilveira, José Franco da2014-07-11T13:51:13Z2014-07-11T13:51:13Z2011SOUZA, Renata Torres de et al. Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi. PLoS One, 6(8): e23042, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/792210.1371/journal.pone.0023042engPublic Library of ScienceGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilInstituto Carlos Chagas. Paraná, BrazilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilBackground The Trypanosoma cruzi genome was sequenced from a hybrid strain (CL Brener). However, high allelic variation and the repetitive nature of the genome have prevented the complete linear sequence of chromosomes being determined. Determining the full complement of chromosomes and establishing syntenic groups will be important in defining the structure of T. cruzi chromosomes. A large amount of information is now available for T. cruzi and Trypanosoma brucei, providing the opportunity to compare and describe the overall patterns of chromosomal evolution in these parasites. Methodology/Principal Findings The genome sizes, repetitive DNA contents, and the numbers and sizes of chromosomes of nine strains of T. cruzi from four lineages (TcI, TcII, TcV and TcVI) were determined. The genome of the TcI group was statistically smaller than other lineages, with the exception of the TcI isolate Tc1161 (José-IMT). Satellite DNA content was correlated with genome size for all isolates, but this was not accompanied by simultaneous amplification of retrotransposons. Regardless of chromosomal polymorphism, large syntenic groups are conserved among T. cruzi lineages. Duplicated chromosome-sized regions were identified and could be retained as paralogous loci, increasing the dosage of several genes. By comparing T. cruzi and T. brucei chromosomes, homologous chromosomal regions in T. brucei were identified. Chromosomes Tb9 and Tb11 of T. brucei share regions of syntenic homology with three and six T. cruzi chromosomal bands, respectively. Conclusions Despite genome size variation and karyotype polymorphism, T. cruzi lineages exhibit conservation of chromosome structure. Several syntenic groups are conserved among all isolates analyzed in this study. The syntenic regions are larger than expected if rearrangements occur randomly, suggesting that they are conserved owing to positive selection. Mapping of the syntenic regions on T. cruzi chromosomal bands provides evidence for the occurrence of fusion and split events involving T. brucei and T. cruzi chromosomes.Trypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi.pdfGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi.pdfapplication/pdf3683413https://www.arca.fiocruz.br/bitstream/icict/7922/1/Genome%20Size%2c%20Karyotype%20Polymorphism%20and%20Chromosomal%20Evolution%20in%20Trypanosoma%20cruzi.pdff01086f4c2818a32183235c62165cd28MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7922/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi.pdf.txtGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi.pdf.txtExtracted texttext/plain14https://www.arca.fiocruz.br/bitstream/icict/7922/3/Genome%20Size%2c%20Karyotype%20Polymorphism%20and%20Chromosomal%20Evolution%20in%20Trypanosoma%20cruzi.pdf.txt06b7e51e8fc077b8c75076712e4dd2b3MD53icict/79222018-04-06 08:48:48.576oai:www.arca.fiocruz.br:icict/7922TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:48:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
title Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
spellingShingle Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
Souza, Renata Torres de,
Trypanosoma cruzi
title_short Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
title_full Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
title_fullStr Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
title_full_unstemmed Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
title_sort Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi
author Souza, Renata Torres de,
author_facet Souza, Renata Torres de,
Lima, Fabio Mitsuo
Barros, Roberto Rudge de Moraes
Cortez, Danielle Rodrigues
Santos, Michele F.
Veas, Esteban Mauricio Cordero
Ruiz, Jerônimo Conceição
Goldenberg, Samuel
Teixeira, Marta Maria Geraldes
Silveira, José Franco da
author_role author
author2 Lima, Fabio Mitsuo
Barros, Roberto Rudge de Moraes
Cortez, Danielle Rodrigues
Santos, Michele F.
Veas, Esteban Mauricio Cordero
Ruiz, Jerônimo Conceição
Goldenberg, Samuel
Teixeira, Marta Maria Geraldes
Silveira, José Franco da
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Renata Torres de,
Lima, Fabio Mitsuo
Barros, Roberto Rudge de Moraes
Cortez, Danielle Rodrigues
Santos, Michele F.
Veas, Esteban Mauricio Cordero
Ruiz, Jerônimo Conceição
Goldenberg, Samuel
Teixeira, Marta Maria Geraldes
Silveira, José Franco da
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
description Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-07-11T13:51:13Z
dc.date.available.fl_str_mv 2014-07-11T13:51:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SOUZA, Renata Torres de et al. Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi. PLoS One, 6(8): e23042, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7922
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0023042
identifier_str_mv SOUZA, Renata Torres de et al. Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi. PLoS One, 6(8): e23042, 2011.
1932-6203
10.1371/journal.pone.0023042
url https://www.arca.fiocruz.br/handle/icict/7922
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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