Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1128/IAI.02103-05 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2795 |
Resumo: | Agradecemos a Lain C. Pontes-de-Carvalho, a Claudia Ida Brodskyn (ambos da FIOCRUZ, Centro de Pesquisa Gonçalo Moniz), e Antonio Rothfuchs (NIH / NIAID) pela revisão cuidadosa e as sugestões para o manuscrito. |
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Pinheiro Junior, Nathanael FHermida, Micely D' El ReiMacedo, Mariana PMengele Junior, José OrivaldoBafica, Andre Luiz BarbosadosSantos, Washington Luis Conrado2011-08-11T15:46:56Z2011-08-11T15:46:56Z2006PINHEIRO JUNIOR, N. F. et al. Leishmania infection impairs beta1-integrin function and chemokine receptor expression in mononuclear phagocytes. Infection and Immunity, v.74, n.7, p.3912–3921, jul. 2006.0019-9567https://www.arca.fiocruz.br/handle/icict/279510.1128/IAI.02103-05Agradecemos a Lain C. Pontes-de-Carvalho, a Claudia Ida Brodskyn (ambos da FIOCRUZ, Centro de Pesquisa Gonçalo Moniz), e Antonio Rothfuchs (NIH / NIAID) pela revisão cuidadosa e as sugestões para o manuscrito.Este estudo foi financiado pela Fundação de Apoio à Pesquisa do Estado da Bahia (FAPESB) e pelo Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / NIAID, National Institutes of Health. Laboratory of Parasitic Diseases. Immunobiology Section. Bethesda, Maryland, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilLeishmania spp. are intracellular parasites that cause lesions in the skin, mucosa, and viscera. We have previously shown that Leishmania infection reduces mononuclear phagocyte adhesion to inflamed connective tissue. In this study, we examined the role of adhesion molecules and chemokines in this process. Infection rate (r 0.826, P 0.003) and parasite burden (r 0.917, P 0.028) negatively correlated to mouse phagocyte adhesion. The decrease (58.7 to 75.0% inhibition, P 0.005) in phagocyte adhesion to connective tissue, induced by Leishmania, occurred as early as 2 h after infection and was maintained for at least 24 h. Interestingly, impairment of cell adhesion was sustained by phagocyte infection, since it was not observed following phagocytosis of killed parasites (cell adhesion varied from 15.2% below to 24.0% above control levels, P > 0.05). In addition, Leishmania infection diminished cell adhesion to fibronectin (54.1 to 96.2%, P < 0.01), collagen (15.7 to 83.7%, P < 0.05), and laminin (59.1 to 82.2%, P < 0.05). The CD11bhi subpopulation was highly infected (49.6 to 97.3%). Calcium and Mg2 replacement by Mn2 , a treatment that is known to induce integrins to a high state of affinity for their receptors, reverted the inhibition in adhesion caused by Leishmania. This reversion was completely blocked by anti-VLA4 antibodies. Furthermore, expression of CCR4 and CCR5, two chemokine receptors implicated in cell adhesion, was found to be downregulated 16 h after infection (2.8 to 4.1 times and 1.9 to 2.8 times, respectively). Together, these results suggest that mechanisms regulating integrin function are implicated in the change of macrophage adhesion in leishmaniasis.engAmerican Society for MicrobiologyLeishmaniaInfecçãoQuimiocinasLeishmaniaInfectionChemokinesAnimaisAntígenos CD29Adesão CelularLeishmania braziliensisLeishmaniose CutâneaMacrófagos PeritoneaisCamundongosCamundongos Endogâmicos BALB CReceptores de QuimiocinasLeishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPinheiro_Hermida_Macedo_etal.pdfPinheiro_Hermida_Macedo_etal.pdfapplication/pdf453616https://www.arca.fiocruz.br/bitstream/icict/2795/1/Pinheiro_Hermida_Macedo_etal.pdf3e5620d500554226936427e6809a7d33MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2795/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTPinheiro_Hermida_Macedo_etal.pdf.txtPinheiro_Hermida_Macedo_etal.pdf.txtExtracted texttext/plain55494https://www.arca.fiocruz.br/bitstream/icict/2795/5/Pinheiro_Hermida_Macedo_etal.pdf.txt47985a0abf7f3ac5eb33cab4d974cf8bMD55THUMBNAILPinheiro_Hermida_Macedo_etal.pdf.jpgPinheiro_Hermida_Macedo_etal.pdf.jpgGenerated Thumbnailimage/jpeg2076https://www.arca.fiocruz.br/bitstream/icict/2795/4/Pinheiro_Hermida_Macedo_etal.pdf.jpge5f01ce7e10b96104886f5d086f8b4e7MD54icict/27952023-03-15 14:34:09.353oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| title |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| spellingShingle |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes Pinheiro Junior, Nathanael F Leishmania Infecção Quimiocinas Leishmania Infection Chemokines Animais Antígenos CD29 Adesão Celular Leishmania braziliensis Leishmaniose Cutânea Macrófagos Peritoneais Camundongos Camundongos Endogâmicos BALB C Receptores de Quimiocinas |
| title_short |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| title_full |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| title_fullStr |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| title_full_unstemmed |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| title_sort |
Leishmania infection impairs β1-integrin function and chemokine receptor expression in mononuclear phagocytes |
| author |
Pinheiro Junior, Nathanael F |
| author_facet |
Pinheiro Junior, Nathanael F Hermida, Micely D' El Rei Macedo, Mariana P Mengele Junior, José Orivaldo Bafica, Andre Luiz Barbosa dosSantos, Washington Luis Conrado |
| author_role |
author |
| author2 |
Hermida, Micely D' El Rei Macedo, Mariana P Mengele Junior, José Orivaldo Bafica, Andre Luiz Barbosa dosSantos, Washington Luis Conrado |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Pinheiro Junior, Nathanael F Hermida, Micely D' El Rei Macedo, Mariana P Mengele Junior, José Orivaldo Bafica, Andre Luiz Barbosa dosSantos, Washington Luis Conrado |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Infecção Quimiocinas |
| topic |
Leishmania Infecção Quimiocinas Leishmania Infection Chemokines Animais Antígenos CD29 Adesão Celular Leishmania braziliensis Leishmaniose Cutânea Macrófagos Peritoneais Camundongos Camundongos Endogâmicos BALB C Receptores de Quimiocinas |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Infection Chemokines |
| dc.subject.decs.pt_BR.fl_str_mv |
Animais Antígenos CD29 Adesão Celular Leishmania braziliensis Leishmaniose Cutânea Macrófagos Peritoneais Camundongos Camundongos Endogâmicos BALB C Receptores de Quimiocinas |
| description |
Agradecemos a Lain C. Pontes-de-Carvalho, a Claudia Ida Brodskyn (ambos da FIOCRUZ, Centro de Pesquisa Gonçalo Moniz), e Antonio Rothfuchs (NIH / NIAID) pela revisão cuidadosa e as sugestões para o manuscrito. |
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2006 |
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2006 |
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2011-08-11T15:46:56Z |
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2011-08-11T15:46:56Z |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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PINHEIRO JUNIOR, N. F. et al. Leishmania infection impairs beta1-integrin function and chemokine receptor expression in mononuclear phagocytes. Infection and Immunity, v.74, n.7, p.3912–3921, jul. 2006. |
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0019-9567 |
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10.1128/IAI.02103-05 |
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PINHEIRO JUNIOR, N. F. et al. Leishmania infection impairs beta1-integrin function and chemokine receptor expression in mononuclear phagocytes. Infection and Immunity, v.74, n.7, p.3912–3921, jul. 2006. 0019-9567 10.1128/IAI.02103-05 |
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American Society for Microbiology |
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American Society for Microbiology |
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