A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/49088 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. |
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Paiva, Milla B.Ribeiro-Romão, Raquel PeralvaResende-Vieira, LarissaBraga-Gomes, ThaisOliveira, Marcia P.Saavedra, Andrea F.Silva-Couto, LuzineiAlbuquerque, Hermano G.Moreira, Otacilio C.Pinto, Eduardo FonsecaDa-Cruz, Alda MariaGomes-Silva, Adriano2021-09-16T13:27:47Z2021-09-16T13:27:47Z2021PAIVA, Milla B. et al. A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model. Frontiers in Immunology, v. 12, p. 1-14, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/4908810.3389/fimmu.2021.656919engFrontiers MediaA Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Rede de Pesquisas em Saúde do Estado do Rio de Janeiro/FAPERJ. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Rede de Pesquisas em Saúde do Estado do Rio de Janeiro/FAPERJ. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia-DMIP. Rio de Janeiro, RJ, Brazil / The National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Micobacterioses. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.The golden hamster is a suitable model for studying cutaneous leishmaniasis (CL) due to Leishmania (Viannia) braziliensis. Immunopathological mechanisms are well established in the L. (L.) major-mouse model, in which IL-4 instructs a Th2 response towards progressive infection. In the present study, we evaluated the natural history of L. braziliensis infection from its first stages up to lesion establishment, with the aim of identifying immunological parameters associated with the disease outcome and parasitism fate. To this end, hamsters infected with 104, 105, or 106 promastigotes were monitored during the first hours (4h, 24h), early (15 days, 30 days) and late (50 days) post-infection (pi) phases. Cytokines, iNOS and arginase gene expression were quantified in the established lesions by reverse transcription-quantitative PCR. Compared to the 105 or 106 groups, 104 animals presented lower lesions sizes, less tissue damage, and lower IgG levels. Basal gene expression in normal skin was high for TGF-β, and intermediary for TNF, IL-6, and IL-4. At 4hpi, no cytokine induction was observed in the 104 group, while an upregulation of IL-6, IL-10, and IL-4 was observed in the 106 group. At 15dpi, lesion appearance was accompanied by an increased expression of all assessed cytokines, markedly in the 105 and 106 groups. Upregulation of all investigated cytokines was observed in the late phase, although less expressive in the 104 group. IFN-γ was the depending variable influencing tissue damage, while IL-6 was associated to parasite load. The network correlating gene expression and clinical and laboratorial parameters indicated inoculum-independent associations at 15 and 30dpi. A strong positive network correlation was observed in the 104 group, but not in the 105 or 106 groups. In conclusion, IL-4, IL-6, IL-10, and TGF-β are linked o L. braziliensis progression. However, a balanced cytokine network is the key for an immune response able to reduce the ongoing infection and reduce pathological damage.Leishmania (Viannia) braziliensisCutaneous lesionsCytokinesDisease controlHamster modeliNOS/arginaseImmune-regulationParasite loadinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/49088/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALA Cytokine_Adriano_Silva_etal_INI_2021.pdfA Cytokine_Adriano_Silva_etal_INI_2021.pdfapplication/pdf2577969https://www.arca.fiocruz.br/bitstream/icict/49088/2/A%20Cytokine_Adriano_Silva_etal_INI_2021.pdf014b4a05e8cb45a264504b93cce7cc83MD52icict/490882023-11-13 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| dc.title.pt_BR.fl_str_mv |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| title |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| spellingShingle |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model Paiva, Milla B. Leishmania (Viannia) braziliensis Cutaneous lesions Cytokines Disease control Hamster model iNOS/arginase Immune-regulation Parasite load |
| title_short |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| title_full |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| title_fullStr |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| title_full_unstemmed |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| title_sort |
A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model |
| author |
Paiva, Milla B. |
| author_facet |
Paiva, Milla B. Ribeiro-Romão, Raquel Peralva Resende-Vieira, Larissa Braga-Gomes, Thais Oliveira, Marcia P. Saavedra, Andrea F. Silva-Couto, Luzinei Albuquerque, Hermano G. Moreira, Otacilio C. Pinto, Eduardo Fonseca Da-Cruz, Alda Maria Gomes-Silva, Adriano |
| author_role |
author |
| author2 |
Ribeiro-Romão, Raquel Peralva Resende-Vieira, Larissa Braga-Gomes, Thais Oliveira, Marcia P. Saavedra, Andrea F. Silva-Couto, Luzinei Albuquerque, Hermano G. Moreira, Otacilio C. Pinto, Eduardo Fonseca Da-Cruz, Alda Maria Gomes-Silva, Adriano |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paiva, Milla B. Ribeiro-Romão, Raquel Peralva Resende-Vieira, Larissa Braga-Gomes, Thais Oliveira, Marcia P. Saavedra, Andrea F. Silva-Couto, Luzinei Albuquerque, Hermano G. Moreira, Otacilio C. Pinto, Eduardo Fonseca Da-Cruz, Alda Maria Gomes-Silva, Adriano |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania (Viannia) braziliensis Cutaneous lesions Cytokines Disease control Hamster model iNOS/arginase Immune-regulation Parasite load |
| topic |
Leishmania (Viannia) braziliensis Cutaneous lesions Cytokines Disease control Hamster model iNOS/arginase Immune-regulation Parasite load |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-09-16T13:27:47Z |
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2021-09-16T13:27:47Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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PAIVA, Milla B. et al. A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model. Frontiers in Immunology, v. 12, p. 1-14, 2021. |
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https://www.arca.fiocruz.br/handle/icict/49088 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2021.656919 |
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PAIVA, Milla B. et al. A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model. Frontiers in Immunology, v. 12, p. 1-14, 2021. 1664-3224 10.3389/fimmu.2021.656919 |
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Frontiers Media |
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Frontiers Media |
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