Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.

Detalhes bibliográficos
Autor(a) principal: Vidal, Daniel Torres Andion
Data de Publicação: 2012
Outros Autores: Fortunato, Gustavo, Klein, Wilfried, Cortizo, L, Vasconcelos, Juliana Fraga, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira, Macambira, Simone Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8575
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil
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spelling Vidal, Daniel Torres AndionFortunato, GustavoKlein, WilfriedCortizo, LVasconcelos, Juliana FragaSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraMacambira, Simone Garcia2014-10-10T16:54:22Z2014-10-10T16:54:22Z2012VIDAL, D. T. A. et al. Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances. Revista Portuguesa de Pneumologia, v. 18, n. 3, p. 128-136, 2012.0873-2159https://www.arca.fiocruz.br/handle/icict/857510.1016/j.rppneu.2011.12.007engElsevier EspañaAlterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, BrasilUniversidade Federal da Bahia. Faculdade de Biologia. Instituto de Biologia. Salvador, BA, Brasil / Instituto de Biociências. Instituto Nacional de Ciência e Tecnologia em Fisiologia Comparada. UNESP. Rio Claro, SP, Brasil / Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Biologia. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil /Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Eletrofisiologia Cardíaca ‘‘Prof Antônio Paes de Carvalho’’. Rio de Janeiro, RJ, BrasilSeveral experimental studies of pulmonary emphysema using animal models have been described in the literature. However, only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema. Additionally, functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema. The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment. This was evaluated by measuring exercise capacity in C57Bl/6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema. Thirty six mice underwent ergometric tests before and 28 days after elastase administration. Pancreatic porcine elastase solution was administered by tracheal puncture, which resulted in a significantly decreased exercise capacity, shown by a shorter distance run (-30.5%) and a lower mean velocity (-15%), as well as in failure to increase the elimination of carbon dioxide. The mean linear intercept increased significantly by 50% in tracheal elastase administration. In conclusion, application of elastase by tracheal function in C57Bl/6 induces emphysema, as validated by morphometric analyses, and resulted in a significantly lower exercise capacity, while resulting in a low mortality rate.Pulmonary empysemaErgometric testPorcine elastaseC57Bl/6Enfisema PulmonarModelos Animais de DoençasPulmão/efeitos de drogasPulmão/patologiaElastase Pancreática/farmacologiaAnimaisFemininoCamundongosCamundongos Endogâmicos C57BLEnfisema Pulmonar/induzido quimicamenteinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8575/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALVidal, D. et al. Alterations in pulmonary. Rev Port Pneumol 2012;p.128.pdfVidal, D. et al. Alterations in pulmonary. Rev Port Pneumol 2012;p.128.pdfapplication/pdf331883https://www.arca.fiocruz.br/bitstream/icict/8575/2/Vidal%2c%20D.%20et%20al.%20Alterations%20in%20pulmonary.%20Rev%20Port%20Pneumol%202012%3bp.128.pdfe051c41aab2c4858f180b5a4a7f36ec6MD52TEXTVidal, D. et al. Alterations in pulmonary. Rev Port Pneumol 2012;p.128.pdf.txtVidal, D. et al. Alterations in pulmonary. Rev Port Pneumol 2012;p.128.pdf.txtExtracted texttext/plain52416https://www.arca.fiocruz.br/bitstream/icict/8575/3/Vidal%2c%20D.%20et%20al.%20Alterations%20in%20pulmonary.%20Rev%20Port%20Pneumol%202012%3bp.128.pdf.txtc156c9c9c544756df98507a053ceb1cbMD53icict/85752023-03-15 14:33:18.877oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
title Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
spellingShingle Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
Vidal, Daniel Torres Andion
Pulmonary empysema
Ergometric test
Porcine elastase
C57Bl/6
Enfisema Pulmonar
Modelos Animais de Doenças
Pulmão/efeitos de drogas
Pulmão/patologia
Elastase Pancreática/farmacologia
Animais
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Enfisema Pulmonar/induzido quimicamente
title_short Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
title_full Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
title_fullStr Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
title_full_unstemmed Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
title_sort Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances.
author Vidal, Daniel Torres Andion
author_facet Vidal, Daniel Torres Andion
Fortunato, Gustavo
Klein, Wilfried
Cortizo, L
Vasconcelos, Juliana Fraga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Macambira, Simone Garcia
author_role author
author2 Fortunato, Gustavo
Klein, Wilfried
Cortizo, L
Vasconcelos, Juliana Fraga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Macambira, Simone Garcia
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vidal, Daniel Torres Andion
Fortunato, Gustavo
Klein, Wilfried
Cortizo, L
Vasconcelos, Juliana Fraga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Macambira, Simone Garcia
dc.subject.en.pt_BR.fl_str_mv Pulmonary empysema
Ergometric test
Porcine elastase
C57Bl/6
Enfisema Pulmonar
topic Pulmonary empysema
Ergometric test
Porcine elastase
C57Bl/6
Enfisema Pulmonar
Modelos Animais de Doenças
Pulmão/efeitos de drogas
Pulmão/patologia
Elastase Pancreática/farmacologia
Animais
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Enfisema Pulmonar/induzido quimicamente
dc.subject.decs.pt_BR.fl_str_mv Modelos Animais de Doenças
Pulmão/efeitos de drogas
Pulmão/patologia
Elastase Pancreática/farmacologia
Animais
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Enfisema Pulmonar/induzido quimicamente
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-10-10T16:54:22Z
dc.date.available.fl_str_mv 2014-10-10T16:54:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv VIDAL, D. T. A. et al. Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances. Revista Portuguesa de Pneumologia, v. 18, n. 3, p. 128-136, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8575
dc.identifier.issn.none.fl_str_mv 0873-2159
dc.identifier.doi.none.fl_str_mv 10.1016/j.rppneu.2011.12.007
identifier_str_mv VIDAL, D. T. A. et al. Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances. Revista Portuguesa de Pneumologia, v. 18, n. 3, p. 128-136, 2012.
0873-2159
10.1016/j.rppneu.2011.12.007
url https://www.arca.fiocruz.br/handle/icict/8575
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier España
publisher.none.fl_str_mv Elsevier España
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