Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8549 http://dx.doi.org/10.1590/S0074-02762013000100011 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil. |
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Zahner, VivianeSilva, Ana Carolina Telles de Carvalho eMoraes, Gabriela Pinhel deMcIntosh, DouglasFilippis, Ivano de2014-10-08T17:14:23Z2014-10-08T17:14:23Z2013ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013.0074-0276https://www.arca.fiocruz.br/handle/icict/8549http://dx.doi.org/10.1590/S0074-02762013000100011engInstituto Oswaldo CruzExtended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia Animal. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species.Bacillus cereusMultiple Locus Sequence TypingVirulence FactorsBacillus cereusTipagem de Sequências MultilocusFatores de VirulênciaBacillus thuringiensisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8549/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMem Inst Oswaldo Cruz_108_65-72.pdfMem Inst Oswaldo Cruz_108_65-72.pdfapplication/pdf549982https://www.arca.fiocruz.br/bitstream/icict/8549/2/Mem%20Inst%20Oswaldo%20Cruz_108_65-72.pdf151d2fbabfd18e15838943595b5b9b1eMD52TEXTMem Inst Oswaldo Cruz_108_65-72.pdf.txtMem Inst Oswaldo Cruz_108_65-72.pdf.txtExtracted texttext/plain41723https://www.arca.fiocruz.br/bitstream/icict/8549/3/Mem%20Inst%20Oswaldo%20Cruz_108_65-72.pdf.txt05b299c76f5d928a18fc469bb3a55106MD53icict/85492018-04-06 08:43:53.031oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.en.fl_str_mv |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
title |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
spellingShingle |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis Zahner, Viviane Bacillus cereus Multiple Locus Sequence Typing Virulence Factors Bacillus cereus Tipagem de Sequências Multilocus Fatores de Virulência Bacillus thuringiensis |
title_short |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
title_full |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
title_fullStr |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
title_full_unstemmed |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
title_sort |
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis |
author |
Zahner, Viviane |
author_facet |
Zahner, Viviane Silva, Ana Carolina Telles de Carvalho e Moraes, Gabriela Pinhel de McIntosh, Douglas Filippis, Ivano de |
author_role |
author |
author2 |
Silva, Ana Carolina Telles de Carvalho e Moraes, Gabriela Pinhel de McIntosh, Douglas Filippis, Ivano de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Zahner, Viviane Silva, Ana Carolina Telles de Carvalho e Moraes, Gabriela Pinhel de McIntosh, Douglas Filippis, Ivano de |
dc.subject.en.en.fl_str_mv |
Bacillus cereus Multiple Locus Sequence Typing Virulence Factors |
topic |
Bacillus cereus Multiple Locus Sequence Typing Virulence Factors Bacillus cereus Tipagem de Sequências Multilocus Fatores de Virulência Bacillus thuringiensis |
dc.subject.decs.pt_BR.fl_str_mv |
Bacillus cereus Tipagem de Sequências Multilocus Fatores de Virulência Bacillus thuringiensis |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Setor de Entomologia Médica Forense. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-10-08T17:14:23Z |
dc.date.available.fl_str_mv |
2014-10-08T17:14:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8549 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762013000100011 |
identifier_str_mv |
ZAHNER, V. et al. Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 65-72, fev. 2013. 0074-0276 |
url |
https://www.arca.fiocruz.br/handle/icict/8549 http://dx.doi.org/10.1590/S0074-02762013000100011 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz |
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Instituto Oswaldo Cruz |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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