A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.

Detalhes bibliográficos
Autor(a) principal: Andrade, Dafne Carvalho
Data de Publicação: 2014
Outros Autores: Borges, Igor Campos, Laitinen, Hanna, Ekström, Nina, Adrian, Peter V, Meinke, Andreas, Barral, Aldina Maria Prado, Carvalho, Cristiana Maria Costa Nascimento, Käyhty, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7876
Resumo: Federal University of Bahia School of Medicine. Salvador, BA, Brasil
id CRUZ_e7e3d0a14383f41a6f64c5164bb7dc4a
oai_identifier_str oai:www.arca.fiocruz.br:icict/7876
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Andrade, Dafne CarvalhoBorges, Igor CamposLaitinen, HannaEkström, NinaAdrian, Peter VMeinke, AndreasBarral, Aldina Maria PradoCarvalho, Cristiana Maria Costa NascimentoKäyhty, Helena2014-07-07T18:32:46Z2014-07-07T18:32:46Z2014ANDRADE, D. C. et al. A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens. Journal of Immunological Methods, v. 405, p. 130-143, 2014.1872-7905https://www.arca.fiocruz.br/handle/icict/787610.1016/j.jim.2014.02.002engElsevier B.VA fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia School of Medicine. Salvador, BA, BrasilFederal University of Bahia School of Medicine. Salvador, BA, BrasilNational Institute for Health and Welfare. Helsinki, FinlandNational Institute for Health and Welfare. Helsinki, FinlandUniversity of the Witwatersrand. DST/NRF Vaccine Preventable Diseases. Respiratory and Meningeal Pathogens Research Unit. Johannesburg, South AfricaValneva Austria. GmbH. Campus Vienna Biocenter. Vienna, Austria.Federal University of Bahia School of Medicine. Pathology Department. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia School of Medicine. Department of Pediatrics. Salvador, BA, Brasil.National Institute for Health and Welfare. Helsinki, Finland.Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are pathogens commonly associated with infectious diseases in childhood. This study aimed to develop a fluorescent multiplexed bead-based immunoassay (FMIA) using recombinant proteins for the quantitation of serum IgG antibodies against these bacteria. Eight pneumococcal proteins (Ply, CbpA, PspA1, PspA2, PcpA, PhtD, SP1732-3 and SP2216-1), 3 proteins of H. influenzae (NTHi Protein D, NTHi0371-1, NTHi0830), and 5 proteins of M. catarrhalis (MC Omp CD, MC_RH4_2506, MC_RH4_1701, MC_RH4_3729-1, MC_RH4_4730) were used to develop the FMIA. Optimal coupling concentrations for each protein, comparison of singleplex and multiplex assays, specificity, reproducibility, and correlation to ELISA for six pneumococcal antigens were determined for validation. FMIA was then used to analyze acute and convalescent paired serum samples of 50 children with non-severe pneumonia. The coupling concentrations varied for different antigens, ranging from 1.6 to 32 μg of protein/million beads. Correlation between singleplexed and multiplexed assays was excellent, with R ≥ 0.987. The FMIA was specific, reaching N92% homologous inhibition for all specificities; heterologous inhibition ≥20% was found only in six cases. The assay was repeatable, with averages of intra-assay variation ≤10.5%, day-to-day variation ≤9.7% and variation between technicians ≤9.1%. Comparison with ELISA for pneumococcal antigens demonstrated good correlation with R ranging from 0.854 (PspA2) to 0.976 (PcpA). The samples from children showed a wide range of antibody concentrations and increases in convalescent samples. In conclusion, the FMIA was sensitive, specific, and repeatable, using small amounts of recombinant proteins and sera to detecAntibodyMultiplexEtiological diagnosisRecombinant proteinsSerologyValidationProteínas de Bactérias/imunologiaHaemophilus influenzae/imunologiaImunoensaio/métodosImunoglobulina G/imunologiaMoraxella (Branhamella) catarrhalis/imunologiaStreptococcus pneumoniae/imunologiaDoença AgudaAnticorpos Antibacterianos/sangueAntígenos de Bactérias/imunologiaCriançaPré-EscolarInfecções Comunitárias Adquiridas/sangueFluorescênciaHumanosImunoglobulina G/sangueLactenteMicroesferasOtite Média/sanguePneumonia/sangueReprodutibilidade dos Testesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAndrade DC A fluorescent ....pdfAndrade DC A fluorescent ....pdfapplication/pdf1567144https://www.arca.fiocruz.br/bitstream/icict/7876/1/Andrade%20DC%20A%20fluorescent%20....pdf90fe5113aef752585c9efef21a402894MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7876/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAndrade DC A fluorescent ....pdf.txtAndrade DC A fluorescent ....pdf.txtExtracted texttext/plain59108https://www.arca.fiocruz.br/bitstream/icict/7876/3/Andrade%20DC%20A%20fluorescent%20....pdf.txt338aad4af1f88d7c76e7d768eff184ccMD53icict/78762023-03-15 14:33:19.661oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
title A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
spellingShingle A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
Andrade, Dafne Carvalho
Antibody
Multiplex
Etiological diagnosis
Recombinant proteins
Serology
Validation
Proteínas de Bactérias/imunologia
Haemophilus influenzae/imunologia
Imunoensaio/métodos
Imunoglobulina G/imunologia
Moraxella (Branhamella) catarrhalis/imunologia
Streptococcus pneumoniae/imunologia
Doença Aguda
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Criança
Pré-Escolar
Infecções Comunitárias Adquiridas/sangue
Fluorescência
Humanos
Imunoglobulina G/sangue
Lactente
Microesferas
Otite Média/sangue
Pneumonia/sangue
Reprodutibilidade dos Testes
title_short A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
title_full A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
title_fullStr A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
title_full_unstemmed A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
title_sort A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens.
author Andrade, Dafne Carvalho
author_facet Andrade, Dafne Carvalho
Borges, Igor Campos
Laitinen, Hanna
Ekström, Nina
Adrian, Peter V
Meinke, Andreas
Barral, Aldina Maria Prado
Carvalho, Cristiana Maria Costa Nascimento
Käyhty, Helena
author_role author
author2 Borges, Igor Campos
Laitinen, Hanna
Ekström, Nina
Adrian, Peter V
Meinke, Andreas
Barral, Aldina Maria Prado
Carvalho, Cristiana Maria Costa Nascimento
Käyhty, Helena
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Dafne Carvalho
Borges, Igor Campos
Laitinen, Hanna
Ekström, Nina
Adrian, Peter V
Meinke, Andreas
Barral, Aldina Maria Prado
Carvalho, Cristiana Maria Costa Nascimento
Käyhty, Helena
dc.subject.en.pt_BR.fl_str_mv Antibody
Multiplex
Etiological diagnosis
Recombinant proteins
Serology
Validation
topic Antibody
Multiplex
Etiological diagnosis
Recombinant proteins
Serology
Validation
Proteínas de Bactérias/imunologia
Haemophilus influenzae/imunologia
Imunoensaio/métodos
Imunoglobulina G/imunologia
Moraxella (Branhamella) catarrhalis/imunologia
Streptococcus pneumoniae/imunologia
Doença Aguda
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Criança
Pré-Escolar
Infecções Comunitárias Adquiridas/sangue
Fluorescência
Humanos
Imunoglobulina G/sangue
Lactente
Microesferas
Otite Média/sangue
Pneumonia/sangue
Reprodutibilidade dos Testes
dc.subject.decs.pt_BR.fl_str_mv Proteínas de Bactérias/imunologia
Haemophilus influenzae/imunologia
Imunoensaio/métodos
Imunoglobulina G/imunologia
Moraxella (Branhamella) catarrhalis/imunologia
Streptococcus pneumoniae/imunologia
Doença Aguda
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Criança
Pré-Escolar
Infecções Comunitárias Adquiridas/sangue
Fluorescência
Humanos
Imunoglobulina G/sangue
Lactente
Microesferas
Otite Média/sangue
Pneumonia/sangue
Reprodutibilidade dos Testes
description Federal University of Bahia School of Medicine. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-07-07T18:32:46Z
dc.date.available.fl_str_mv 2014-07-07T18:32:46Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, D. C. et al. A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens. Journal of Immunological Methods, v. 405, p. 130-143, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7876
dc.identifier.issn.none.fl_str_mv 1872-7905
dc.identifier.doi.none.fl_str_mv 10.1016/j.jim.2014.02.002
identifier_str_mv ANDRADE, D. C. et al. A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens. Journal of Immunological Methods, v. 405, p. 130-143, 2014.
1872-7905
10.1016/j.jim.2014.02.002
url https://www.arca.fiocruz.br/handle/icict/7876
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier B.V
publisher.none.fl_str_mv Elsevier B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7876/1/Andrade%20DC%20A%20fluorescent%20....pdf
https://www.arca.fiocruz.br/bitstream/icict/7876/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7876/3/Andrade%20DC%20A%20fluorescent%20....pdf.txt
bitstream.checksum.fl_str_mv 90fe5113aef752585c9efef21a402894
7d48279ffeed55da8dfe2f8e81f3b81f
338aad4af1f88d7c76e7d768eff184cc
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324618351607808

Registros relacionados