Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Zilton Farias Meira de
Data de Publicação: 2015
Outros Autores: Müller, Sabina, Guipouy, Delphine, Yu, Wong, Christophe, Claire, Gadat, Sébastien, Valitutti, Salvatore, Dupré, Loïc
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43752
Resumo: Supplemental Information includes Supplemental Statistical Methods, four fig-ures, and six movies and can be found with this article online athttp:10.1016/j.celrep.2015.05.002.//dx.doi.org/
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spelling Vasconcelos, Zilton Farias Meira deMüller, SabinaGuipouy, DelphineYu, WongChristophe, ClaireGadat, SébastienValitutti, SalvatoreDupré, Loïc2020-09-30T18:53:37Z2020-09-30T18:53:37Z2015VASCONCELOS, Zilton Farias Meira de et al. Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing. Cell Reports, v. 11, n. 9, p. 1474-1485, Jun 2015.2211-1247https://www.arca.fiocruz.br/handle/icict/4375210.1016/j.celrep.2015.05.002Supplemental Information includes Supplemental Statistical Methods, four fig-ures, and six movies and can be found with this article online athttp:10.1016/j.celrep.2015.05.002.//dx.doi.org/1K08DK093709-01/DK/NIDDK NIH HHS/United StatesFundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Rio de Janeiro, RJ, Brasil / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. Toulouse. France.Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. Toulouse. France.Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. Toulouse. France.Stanford University School of Medicine. Department of Microbiology and Immunology. Stanford, CA. USA.Université Toulouse III Paul-Sabatier. Institut de Mathématiques de Toulouse. Toulouse. France.Université Toulouse. Toulouse School of Economics, Toulouse. France.INSERM. Centre de Physiopathologie de Toulouse. Toulouse. France / Université Toulouse III Paul-Sabatier. Toulouse. France.INSERM. Centre de Physiopathologie de Toulouse. Toulouse. France / Université Toulouse III Paul-Sabatier. Toulouse. France.The killing of antigen-bearing cells by clonal populations of cytotoxic T lymphocytes (CTLs) is thought to be a rapid phenomenon executed uniformly by individual CTLs. We combined bulk and single-CTL killing assays over a prolonged time period to provide the killing statistics of clonal human CTLs against an excess of target cells. Our data reveal efficiency in sustained killing at the population level, which relied on a highly heterogeneous multiple killing performance at the individual level. Although intraclonal functional heterogeneity was a stable trait in clonal populations, it was reset in the progeny of individual CTLs. In-depth mathematical analysis of individual CTL killing data revealed a substantial proportion of high-rate killer CTLs with burst killing activity. Importantly, such activity was delayed and required activation with strong antigenic stimulation. Our study implies that functional heterogeneity allows CTL populations to calibrate prolonged cytotoxic activity to the size of target cell populations.engCell PressCytotoxicity, Immunologic / immunologyFlow CytometryHumansMicroscopy, ConfocalModels, TheoreticalT-Lymphocytes, Cytotoxic / immunologyIndividual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
title Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
spellingShingle Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
Vasconcelos, Zilton Farias Meira de
Cytotoxicity, Immunologic / immunology
Flow Cytometry
Humans
Microscopy, Confocal
Models, Theoretical
T-Lymphocytes, Cytotoxic / immunology
title_short Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
title_full Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
title_fullStr Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
title_full_unstemmed Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
title_sort Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
author Vasconcelos, Zilton Farias Meira de
author_facet Vasconcelos, Zilton Farias Meira de
Müller, Sabina
Guipouy, Delphine
Yu, Wong
Christophe, Claire
Gadat, Sébastien
Valitutti, Salvatore
Dupré, Loïc
author_role author
author2 Müller, Sabina
Guipouy, Delphine
Yu, Wong
Christophe, Claire
Gadat, Sébastien
Valitutti, Salvatore
Dupré, Loïc
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, Zilton Farias Meira de
Müller, Sabina
Guipouy, Delphine
Yu, Wong
Christophe, Claire
Gadat, Sébastien
Valitutti, Salvatore
Dupré, Loïc
dc.subject.mesh.pt_BR.fl_str_mv Cytotoxicity, Immunologic / immunology
Flow Cytometry
Humans
Microscopy, Confocal
Models, Theoretical
T-Lymphocytes, Cytotoxic / immunology
topic Cytotoxicity, Immunologic / immunology
Flow Cytometry
Humans
Microscopy, Confocal
Models, Theoretical
T-Lymphocytes, Cytotoxic / immunology
description Supplemental Information includes Supplemental Statistical Methods, four fig-ures, and six movies and can be found with this article online athttp:10.1016/j.celrep.2015.05.002.//dx.doi.org/
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2020-09-30T18:53:37Z
dc.date.available.fl_str_mv 2020-09-30T18:53:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VASCONCELOS, Zilton Farias Meira de et al. Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing. Cell Reports, v. 11, n. 9, p. 1474-1485, Jun 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43752
dc.identifier.issn.pt_BR.fl_str_mv 2211-1247
dc.identifier.doi.none.fl_str_mv 10.1016/j.celrep.2015.05.002
identifier_str_mv VASCONCELOS, Zilton Farias Meira de et al. Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing. Cell Reports, v. 11, n. 9, p. 1474-1485, Jun 2015.
2211-1247
10.1016/j.celrep.2015.05.002
url https://www.arca.fiocruz.br/handle/icict/43752
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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