Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23260 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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Lyra, Juliana Maria Azevedo deMaruza, MagdaVerza, MirelaCarneiro, Maria MadileuzaAlbuquerque, Maria de Fátima Pessoa Militão deRossetti, Maria LúciaXimenes, RicardoBraga, Maria CynthiaLucena-Silva, Norma2017-11-17T14:38:38Z2017-11-17T14:38:38Z2014LYRA, uliana Maria Azevedo de et al. Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 6, p. 805–813, set. 2014.1678-8060https://www.arca.fiocruz.br/handle/icict/2326010.1590/0074-0276130542engtuberculosePaucibacilarReação em Cadeia da Polimerase em Tempo Realtuberculosispaucibacillaryreal-time PCRInfecções por HIVsangueHospedeiro ImunocomprometidoMycobacterium tuberculosisTécnicas de Diagnóstico MolecularmétodosEscarromicrobiologiaTuberculose PulmonardiagnósticoCarga BacterianaCoinfecçãoPrimers do DNAHIVPulmãomicrobiologiaMycobacterium tuberculosiscrescimento & desenvolvimentoKit de Reagentes para DiagnósticonormasReação em Cadeia da Polimerase em Tempo RealmétodosTuberculose PulmonarsangueSensibilidade e EspecificidadeEvaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.The present study analysed the concordance among four different molecular diagnostic methods for tuberculosis (TB) in pulmonary and blood samples from immunocompromised patients. A total of 165 blood and 194 sputum samples were collected from 181 human immunodeficiency virus (HIV)-infected patients with upper respiratory complaints, regardless of suspicious for TB. The samples were submitted for smear microscopy, culture and molecular tests: a laboratory-developed conventional polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR) and the Gen-Probe and Detect-TB Ampligenix kits. The samples were handled blindly by all the technicians involved, from sample processing to results analysis. For sputum, the sensitivity and specificity were 100% and 96.7% for qPCR, 81.8% and 94.5% for Gen-Probe and 100% and 66.3% for Detect-TB, respectively. qPCR presented the best concordance with sputum culture [kappa (k) = 0.864)], followed by Gen-Probe (k = 0.682). For blood samples, qPCR showed 100% sensitivity and 92.3% specificity, with a substantial correlation with sputum culture (k = 0.754) and with the qPCR results obtained from sputum of the corresponding patient (k = 0.630). Conventional PCR demonstrated the worst results for sputa and blood, with a sensitivity of 100% vs. 88.9% and a specificity of 46.3% vs. 32%, respectively. Commercial or laboratory-developed molecular assays can overcome the difficulties in the diagnosis of TB in paucibacillary patients using conventional methods available in most laboratories.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23260/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALART. Evaluation of four molecular - LYRA.pdfART. 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| dc.title.pt_BR.fl_str_mv |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| title |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| spellingShingle |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients Lyra, Juliana Maria Azevedo de tuberculose Paucibacilar Reação em Cadeia da Polimerase em Tempo Real tuberculosis paucibacillary real-time PCR Infecções por HIV sangue Hospedeiro Imunocomprometido Mycobacterium tuberculosis Técnicas de Diagnóstico Molecular métodos Escarro microbiologia Tuberculose Pulmonar diagnóstico Carga Bacteriana Coinfecção Primers do DNA HIV Pulmão microbiologia Mycobacterium tuberculosis crescimento & desenvolvimento Kit de Reagentes para Diagnóstico normas Reação em Cadeia da Polimerase em Tempo Real métodos Tuberculose Pulmonar sangue Sensibilidade e Especificidade |
| title_short |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| title_full |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| title_fullStr |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| title_full_unstemmed |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| title_sort |
Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients |
| author |
Lyra, Juliana Maria Azevedo de |
| author_facet |
Lyra, Juliana Maria Azevedo de Maruza, Magda Verza, Mirela Carneiro, Maria Madileuza Albuquerque, Maria de Fátima Pessoa Militão de Rossetti, Maria Lúcia Ximenes, Ricardo Braga, Maria Cynthia Lucena-Silva, Norma |
| author_role |
author |
| author2 |
Maruza, Magda Verza, Mirela Carneiro, Maria Madileuza Albuquerque, Maria de Fátima Pessoa Militão de Rossetti, Maria Lúcia Ximenes, Ricardo Braga, Maria Cynthia Lucena-Silva, Norma |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lyra, Juliana Maria Azevedo de Maruza, Magda Verza, Mirela Carneiro, Maria Madileuza Albuquerque, Maria de Fátima Pessoa Militão de Rossetti, Maria Lúcia Ximenes, Ricardo Braga, Maria Cynthia Lucena-Silva, Norma |
| dc.subject.other.pt_BR.fl_str_mv |
tuberculose Paucibacilar Reação em Cadeia da Polimerase em Tempo Real |
| topic |
tuberculose Paucibacilar Reação em Cadeia da Polimerase em Tempo Real tuberculosis paucibacillary real-time PCR Infecções por HIV sangue Hospedeiro Imunocomprometido Mycobacterium tuberculosis Técnicas de Diagnóstico Molecular métodos Escarro microbiologia Tuberculose Pulmonar diagnóstico Carga Bacteriana Coinfecção Primers do DNA HIV Pulmão microbiologia Mycobacterium tuberculosis crescimento & desenvolvimento Kit de Reagentes para Diagnóstico normas Reação em Cadeia da Polimerase em Tempo Real métodos Tuberculose Pulmonar sangue Sensibilidade e Especificidade |
| dc.subject.en.pt_BR.fl_str_mv |
tuberculosis paucibacillary real-time PCR |
| dc.subject.decs.pt_BR.fl_str_mv |
Infecções por HIV sangue Hospedeiro Imunocomprometido Mycobacterium tuberculosis Técnicas de Diagnóstico Molecular métodos Escarro microbiologia Tuberculose Pulmonar diagnóstico Carga Bacteriana Coinfecção Primers do DNA HIV Pulmão microbiologia Mycobacterium tuberculosis crescimento & desenvolvimento Kit de Reagentes para Diagnóstico normas Reação em Cadeia da Polimerase em Tempo Real métodos Tuberculose Pulmonar sangue Sensibilidade e Especificidade |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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2014 |
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2014 |
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2017-11-17T14:38:38Z |
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2017-11-17T14:38:38Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LYRA, uliana Maria Azevedo de et al. Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 6, p. 805–813, set. 2014. |
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https://www.arca.fiocruz.br/handle/icict/23260 |
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1678-8060 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0074-0276130542 |
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LYRA, uliana Maria Azevedo de et al. Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 6, p. 805–813, set. 2014. 1678-8060 10.1590/0074-0276130542 |
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https://www.arca.fiocruz.br/handle/icict/23260 |
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eng |
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