Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples

Detalhes bibliográficos
Autor(a) principal: Oliveira, Luciana Bonome Zeminian de
Data de Publicação: 2023
Outros Autores: Coletta, Amanda Manoel Della, Gardizani, Taiane Priscila, Garces, Hans Garcia, Bagagli, Eduardo, Trilles, Luciana, Barrozo, Ligia Vizeu, Marques, Sílvio de Alencar, De Faveri, Julio, Dias-Melicio, Luciane Alarcão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58978
Resumo: This work was supported by research funding from São Paulo Research Foundation (FAPESP) through the Ph.D. fellowship (FAPESP 2013/24877-9). The funders had no role in study design, data collection, and analysis, the decision to publish, or preparation of the manuscript.
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spelling Oliveira, Luciana Bonome Zeminian deColetta, Amanda Manoel DellaGardizani, Taiane PriscilaGarces, Hans GarciaBagagli, EduardoTrilles, LucianaBarrozo, Ligia VizeuMarques, Sílvio de AlencarDe Faveri, JulioDias-Melicio, Luciane Alarcão2023-06-08T00:48:39Z2023-06-08T00:48:39Z2023OLIVEIRA, Luciana Bonome Zeminian de et al. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples. Microorganisms, v. 11, n. 3, p. 1-13, Feb. 2023.2076-2607https://www.arca.fiocruz.br/handle/icict/5897810.3390/microorganisms110305622076-2607This work was supported by research funding from São Paulo Research Foundation (FAPESP) through the Ph.D. fellowship (FAPESP 2013/24877-9). The funders had no role in study design, data collection, and analysis, the decision to publish, or preparation of the manuscript.São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil.São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil.São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil.São Paulo State University. Institute of Biosciences. Department of Chemical and Biological Sciences. Botucatu, SP, Brazil.São Paulo State University. Institute of Biosciences. Department of Chemical and Biological Sciences. Botucatu, SP, Brazil.Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Disease. Rio de Janeiro, RJ, Brazil.University of São Paulo. Literature and Human Sciences. Department of Geography, School of Philosophy. São Paulo, SP, Brazil.São Paulo State University. Department of Dermatology, Medical School of Botucatu. Botucatu, SP, Brazil.São Paulo State University. Medical School of Botucatu. Department of Pathology. Botucatu, SP, Brazil.São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil / São Paulo State University. Medical School of Botucatu. Department of Pathology. Botucatu, SP, Brazil.Paracoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: P. brasiliensis (S1), P. americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients' biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu-UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate Paracoccidioides species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of P. brasiliensis. The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore P. brasiliensis. This is important data, demonstrating the predominance of this species in the São Paulo State region.engMDPIMolecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParacoccidioides brasiliensisParacoccidioides species complexMolecular BiologyMolecular phylogenetic analysisParacoccidiodomycosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58978/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Luciana_Oliveira_etal_INI_2023.pdfve_Luciana_Oliveira_etal_INI_2023.pdfapplication/pdf1573160https://www.arca.fiocruz.br/bitstream/icict/58978/2/ve_Luciana_Oliveira_etal_INI_2023.pdf0f3245017e16b23d9ac8c0c0175b16b8MD52icict/589782023-06-07 21:48:40.752oai:www.arca.fiocruz.br:icict/58978Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório 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dc.title.en_US.fl_str_mv Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
title Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
spellingShingle Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
Oliveira, Luciana Bonome Zeminian de
Paracoccidioides brasiliensis
Paracoccidioides species complex
Molecular Biology
Molecular phylogenetic analysis
Paracoccidiodomycosis
title_short Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
title_full Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
title_fullStr Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
title_full_unstemmed Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
title_sort Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
author Oliveira, Luciana Bonome Zeminian de
author_facet Oliveira, Luciana Bonome Zeminian de
Coletta, Amanda Manoel Della
Gardizani, Taiane Priscila
Garces, Hans Garcia
Bagagli, Eduardo
Trilles, Luciana
Barrozo, Ligia Vizeu
Marques, Sílvio de Alencar
De Faveri, Julio
Dias-Melicio, Luciane Alarcão
author_role author
author2 Coletta, Amanda Manoel Della
Gardizani, Taiane Priscila
Garces, Hans Garcia
Bagagli, Eduardo
Trilles, Luciana
Barrozo, Ligia Vizeu
Marques, Sílvio de Alencar
De Faveri, Julio
Dias-Melicio, Luciane Alarcão
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Luciana Bonome Zeminian de
Coletta, Amanda Manoel Della
Gardizani, Taiane Priscila
Garces, Hans Garcia
Bagagli, Eduardo
Trilles, Luciana
Barrozo, Ligia Vizeu
Marques, Sílvio de Alencar
De Faveri, Julio
Dias-Melicio, Luciane Alarcão
dc.subject.en.en_US.fl_str_mv Paracoccidioides brasiliensis
Paracoccidioides species complex
Molecular Biology
Molecular phylogenetic analysis
Paracoccidiodomycosis
topic Paracoccidioides brasiliensis
Paracoccidioides species complex
Molecular Biology
Molecular phylogenetic analysis
Paracoccidiodomycosis
description This work was supported by research funding from São Paulo Research Foundation (FAPESP) through the Ph.D. fellowship (FAPESP 2013/24877-9). The funders had no role in study design, data collection, and analysis, the decision to publish, or preparation of the manuscript.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-06-08T00:48:39Z
dc.date.available.fl_str_mv 2023-06-08T00:48:39Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Luciana Bonome Zeminian de et al. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples. Microorganisms, v. 11, n. 3, p. 1-13, Feb. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58978
dc.identifier.issn.en_US.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/microorganisms11030562
dc.identifier.eissn.none.fl_str_mv 2076-2607
identifier_str_mv OLIVEIRA, Luciana Bonome Zeminian de et al. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples. Microorganisms, v. 11, n. 3, p. 1-13, Feb. 2023.
2076-2607
10.3390/microorganisms11030562
url https://www.arca.fiocruz.br/handle/icict/58978
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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https://www.arca.fiocruz.br/bitstream/icict/58978/2/ve_Luciana_Oliveira_etal_INI_2023.pdf
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