Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27806 |
Resumo: | Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil. |
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Robaina, T. F.Valladares, C. P.Tavares, D. S.Napolitano, W. C.Silva, L. E..Dias, E. P.Leite, José Paulo G.2018-08-02T16:11:07Z2018-08-02T16:11:07Z2008ROBAÍNA, T. F. Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients. Mem. Inst. Oswaldo Cruz, v.103, n.4, June 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/278061678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruzvírus Epstein-Barrleucoplasia pilosa oralHIV-1Pacientes soropositivos para o HIV-1EBV-1EBV-2oral hairy leukoplakiaHIV-1EBNA-2 geneHIV-1 seropositive patientsPolymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Matemática. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ. Brasil.The Epstein-Barr virus (EBV) is the etiological agent of oral hairy leukoplakia (OHL), an oral lesion with important diagnostic and prognostic value in acquired immunodeficiency disease syndrome. The two EBV genotypes, EBV-1 and EBV-2, can be distinguished by divergent gene sequences encoding the EBNA-2, 3A, 3B, and 3C proteins. The purpose of this study was to identify the EBV genotype prevalent in 53 samples of scrapings from the lateral border of the tongue of HIV-1 seropositive patients, with and without OHL, and to correlate the genotypes with presence of clinical or subclinical OHL with the clinic data collected. EBV-1 and EBV-2 were identified through PCR and Nested-PCR based on sequence differences of the EBNA-2 gene. EBV-1 was identified in the 31 samples (15 without OHL, 7 with clinical OHL and 9 with subclinical OHL), EBV-2 in 12 samples (10 without OHL, 1 with clinical and 1 subclinical OHL), and a mixed infection in 10 samples (2 without OHL, 3 with clinical and 5 with subclinical OHL). The presence of EBV-1 was higher in women, but a significant statistical result relating one the EBV genotypes to the development of OHL was not found. We conclude that the oral epithelium in HIV-1 seropositive patients can be infected by EBV-1, EBV-2 or by a mixed viral population.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27806/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjosepaulo2_leite_etal_IOC_2008.pdfjosepaulo2_leite_etal_IOC_2008.pdfapplication/pdf341413https://www.arca.fiocruz.br/bitstream/icict/27806/2/josepaulo2_leite_etal_IOC_2008.pdf20eb7b8caaeb0cd37888645cbdc67bdcMD52TEXTjosepaulo2_leite_etal_IOC_2008.pdf.txtjosepaulo2_leite_etal_IOC_2008.pdf.txtExtracted texttext/plain30865https://www.arca.fiocruz.br/bitstream/icict/27806/3/josepaulo2_leite_etal_IOC_2008.pdf.txt8296e6c0ae9a492796796ef87aa786c8MD53icict/278062018-08-15 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| dc.title.pt_BR.fl_str_mv |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| title |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| spellingShingle |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients Robaina, T. F. vírus Epstein-Barr leucoplasia pilosa oral HIV-1 Pacientes soropositivos para o HIV-1 EBV-1 EBV-2 oral hairy leukoplakia HIV-1 EBNA-2 gene HIV-1 seropositive patients |
| title_short |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| title_full |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| title_fullStr |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| title_full_unstemmed |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| title_sort |
Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients |
| author |
Robaina, T. F. |
| author_facet |
Robaina, T. F. Valladares, C. P. Tavares, D. S. Napolitano, W. C. Silva, L. E.. Dias, E. P. Leite, José Paulo G. |
| author_role |
author |
| author2 |
Valladares, C. P. Tavares, D. S. Napolitano, W. C. Silva, L. E.. Dias, E. P. Leite, José Paulo G. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Robaina, T. F. Valladares, C. P. Tavares, D. S. Napolitano, W. C. Silva, L. E.. Dias, E. P. Leite, José Paulo G. |
| dc.subject.other.pt_BR.fl_str_mv |
vírus Epstein-Barr leucoplasia pilosa oral HIV-1 Pacientes soropositivos para o HIV-1 |
| topic |
vírus Epstein-Barr leucoplasia pilosa oral HIV-1 Pacientes soropositivos para o HIV-1 EBV-1 EBV-2 oral hairy leukoplakia HIV-1 EBNA-2 gene HIV-1 seropositive patients |
| dc.subject.en.pt_BR.fl_str_mv |
EBV-1 EBV-2 oral hairy leukoplakia HIV-1 EBNA-2 gene HIV-1 seropositive patients |
| description |
Universidade Federal Fluminense. Instituto de Matemática. Programa de Pós-Graduação em Patologia. Niterói, RJ, Brasil. |
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2008 |
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2008 |
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2018-08-02T16:11:07Z |
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2018-08-02T16:11:07Z |
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info:eu-repo/semantics/article |
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article |
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ROBAÍNA, T. F. Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients. Mem. Inst. Oswaldo Cruz, v.103, n.4, June 2008. |
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https://www.arca.fiocruz.br/handle/icict/27806 |
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0074-0276 |
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1678-8060 |
| identifier_str_mv |
ROBAÍNA, T. F. Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients. Mem. Inst. Oswaldo Cruz, v.103, n.4, June 2008. 0074-0276 1678-8060 |
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https://www.arca.fiocruz.br/handle/icict/27806 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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