Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms

Detalhes bibliográficos
Autor(a) principal: Corrêa, José R.
Data de Publicação: 2007
Outros Autores: Atella, Georgia C., Vargas, Camila, Soares, Maurilio J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39328
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.
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spelling Corrêa, José R.Atella, Georgia C.Vargas, CamilaSoares, Maurilio J.2020-01-20T19:05:31Z2020-01-20T19:05:31Z2007CORRÊA, José R. et al. Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 7, p. 871-876, Nov. 2007.0074-0276https://www.arca.fiocruz.br/handle/icict/3932810.1590/s0074-027620070050001171678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.EndocitoseMembrana resistente a detergenteTrypanosoma cruziUltraestruturaFormas de epimastigoteCytopharynxDetergent-resistant membraneEndocytosisTrypanosoma cruziUltrastructureEpimastigote formsTransferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote formsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Uptake of transferrin by epimastigote forms of the protozoan Trypanosoma cruzi occurs mainly through a cytostome/ cytopharynx, via uncoated endocytic vesicles that bud off from the bottom of the cytopharynx. We have here examined whether detergent-resistant membrane (DRM) domains might be involved in this process. Purified whole cell membrane fractions were assayed for cholesterol levels and used in dot blot analyses. Detergent-resistant membrane markers (cholera B toxin and anti-flotillin-1 antibody) presented positive reaction by dot blots in cholesterol-rich/ protein-poor membrane sub-fractions. The positive dot blot fraction was submitted to lipid composition analysis, showing composition similar to that of raft fractions described for other eukaryotic cells. Immunofluorescence assays allowed the localization of punctual positive signal for flotillin-1, matching the precise cytostome/ cytopharynx location. These data were confirmed by immunofluorescence assays with the co-localization of flotillin-1 and the transferrin uptake site. Our data suggest that DRM domains occur and are integrated at the cytostome/ cytopharynx of T. cruzi epimastigotes, being the main route for transferrin uptake.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39328/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdfJoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdfapplication/pdf224585https://www.arca.fiocruz.br/bitstream/icict/39328/2/JoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdf41db67e0dd2de2dce31de94a74ebf4d1MD52TEXTJoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdf.txtJoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdf.txtExtracted texttext/plain30919https://www.arca.fiocruz.br/bitstream/icict/39328/3/JoseRCorrea_GeorgiaAtella_etal_IOC_2007.pdf.txt60b80ab2832cf49370f3e2b827f27cedMD53icict/393282020-01-30 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dc.title.pt_BR.fl_str_mv Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
title Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
spellingShingle Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
Corrêa, José R.
Endocitose
Membrana resistente a detergente
Trypanosoma cruzi
Ultraestrutura
Formas de epimastigote
Cytopharynx
Detergent-resistant membrane
Endocytosis
Trypanosoma cruzi
Ultrastructure
Epimastigote forms
title_short Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
title_full Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
title_fullStr Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
title_full_unstemmed Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
title_sort Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
author Corrêa, José R.
author_facet Corrêa, José R.
Atella, Georgia C.
Vargas, Camila
Soares, Maurilio J.
author_role author
author2 Atella, Georgia C.
Vargas, Camila
Soares, Maurilio J.
author2_role author
author
author
dc.contributor.author.fl_str_mv Corrêa, José R.
Atella, Georgia C.
Vargas, Camila
Soares, Maurilio J.
dc.subject.other.pt_BR.fl_str_mv Endocitose
Membrana resistente a detergente
Trypanosoma cruzi
Ultraestrutura
Formas de epimastigote
topic Endocitose
Membrana resistente a detergente
Trypanosoma cruzi
Ultraestrutura
Formas de epimastigote
Cytopharynx
Detergent-resistant membrane
Endocytosis
Trypanosoma cruzi
Ultrastructure
Epimastigote forms
dc.subject.en.pt_BR.fl_str_mv Cytopharynx
Detergent-resistant membrane
Endocytosis
Trypanosoma cruzi
Ultrastructure
Epimastigote forms
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2020-01-20T19:05:31Z
dc.date.available.fl_str_mv 2020-01-20T19:05:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CORRÊA, José R. et al. Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 7, p. 871-876, Nov. 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39328
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762007005000117
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CORRÊA, José R. et al. Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 7, p. 871-876, Nov. 2007.
0074-0276
10.1590/s0074-02762007005000117
1678-8060
url https://www.arca.fiocruz.br/handle/icict/39328
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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