Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10113 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. |
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Silva, Elisabeth Martins daGuillermo, Landi Veivi CostillaGomes, Flávia Lima RibeiroMeis, Juliana DeNunes, Marise PinheiroSenra, Juliana Fraga VasconcelosSoares, Milena Botelho PereiraReis, George Alexandre dosLopes, Marcela de Freitas2015-04-22T18:34:19Z2015-04-22T18:34:19Z2007SILVA, E. M. et al. Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection. European Joural of Immunology, v. 37, n. 3, p. 738-746, 2007.0014-2980https://www.arca.fiocruz.br/handle/icict/1011310.1002/eji.200636790engWiley-VCH Verlag GmbH & CoCaspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.In experimental Chagas' disease, lymphocytes from mice infected with Trypanosoma cruzi show increased apoptosis in vivo and in vitro. Treatment with a pan-caspase blocker peptide inhibited expression of the active form of effector caspase-3 in vitro and rescued both B and T cells from cell death. Injection of the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethyl ketone, but not a control peptide, reduced parasitemia and lymphocyte apoptosis in T. cruzi-infected mice. Moreover, treatment with caspase inhibitor throughout acute infection increased the absolute numbers of B and T cells in the spleen and lymph nodes, without affecting cell infiltrates in the heart. Following treatment, we found increased accumulation of memory/activated CD4 and CD8 T cells, and secretion of IFN-gamma by splenocytes stimulated with T. cruzi antigens. Caspase inhibition in the course of infection reduced the intracellular load of parasites in peritoneal macrophages, and increased the production of TNF-alpha and nitric oxide upon activation in vitro. Our results indicate that inhibition of caspases with a pan-caspase blocker peptide improves protective type-1 immune responses to T. cruzi infection. We suggest that mechanisms of apoptosis are potential therapeutic targets in Chagas' disease.ApoptosisCaspasesInfectionLymphocyteTrypanosoma cruziApoptose/imunologiaInibidores de CaspaseDoença de Chagas/imunologiaDoença de Chagas/patologiaLinfócitos/enzimologiaTrypanosoma cruzi/imunologiaClorometilcetonas de Aminoácidos/farmacologiaAnimaisApoptose/efeitos de drogasDoença de Chagas/quimioterapiaLinfócitos/efeitos de drogasMasculinoCamundongosCamundongos Endogâmicos BALB Cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10113/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALSilva E M Caspase inhibition....pdfSilva E M Caspase inhibition....pdfapplication/pdf353519https://www.arca.fiocruz.br/bitstream/icict/10113/2/Silva%20E%20M%20Caspase%20inhibition....pdf68b3cc8f4df343863de5c4a9370a4777MD52TEXTSilva E M Caspase inhibition....pdf.txtSilva E M Caspase inhibition....pdf.txtExtracted texttext/plain42191https://www.arca.fiocruz.br/bitstream/icict/10113/3/Silva%20E%20M%20Caspase%20inhibition....pdf.txt9603ff7f97092f69c2713fb926c6c759MD53icict/101132023-03-15 14:33:14.649oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| title |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| spellingShingle |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection Silva, Elisabeth Martins da Apoptosis Caspases Infection Lymphocyte Trypanosoma cruzi Apoptose/imunologia Inibidores de Caspase Doença de Chagas/imunologia Doença de Chagas/patologia Linfócitos/enzimologia Trypanosoma cruzi/imunologia Clorometilcetonas de Aminoácidos/farmacologia Animais Apoptose/efeitos de drogas Doença de Chagas/quimioterapia Linfócitos/efeitos de drogas Masculino Camundongos Camundongos Endogâmicos BALB C |
| title_short |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| title_full |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| title_fullStr |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| title_full_unstemmed |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| title_sort |
Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection |
| author |
Silva, Elisabeth Martins da |
| author_facet |
Silva, Elisabeth Martins da Guillermo, Landi Veivi Costilla Gomes, Flávia Lima Ribeiro Meis, Juliana De Nunes, Marise Pinheiro Senra, Juliana Fraga Vasconcelos Soares, Milena Botelho Pereira Reis, George Alexandre dos Lopes, Marcela de Freitas |
| author_role |
author |
| author2 |
Guillermo, Landi Veivi Costilla Gomes, Flávia Lima Ribeiro Meis, Juliana De Nunes, Marise Pinheiro Senra, Juliana Fraga Vasconcelos Soares, Milena Botelho Pereira Reis, George Alexandre dos Lopes, Marcela de Freitas |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Elisabeth Martins da Guillermo, Landi Veivi Costilla Gomes, Flávia Lima Ribeiro Meis, Juliana De Nunes, Marise Pinheiro Senra, Juliana Fraga Vasconcelos Soares, Milena Botelho Pereira Reis, George Alexandre dos Lopes, Marcela de Freitas |
| dc.subject.en.pt_BR.fl_str_mv |
Apoptosis Caspases Infection Lymphocyte Trypanosoma cruzi |
| topic |
Apoptosis Caspases Infection Lymphocyte Trypanosoma cruzi Apoptose/imunologia Inibidores de Caspase Doença de Chagas/imunologia Doença de Chagas/patologia Linfócitos/enzimologia Trypanosoma cruzi/imunologia Clorometilcetonas de Aminoácidos/farmacologia Animais Apoptose/efeitos de drogas Doença de Chagas/quimioterapia Linfócitos/efeitos de drogas Masculino Camundongos Camundongos Endogâmicos BALB C |
| dc.subject.decs.pt_BR.fl_str_mv |
Apoptose/imunologia Inibidores de Caspase Doença de Chagas/imunologia Doença de Chagas/patologia Linfócitos/enzimologia Trypanosoma cruzi/imunologia Clorometilcetonas de Aminoácidos/farmacologia Animais Apoptose/efeitos de drogas Doença de Chagas/quimioterapia Linfócitos/efeitos de drogas Masculino Camundongos Camundongos Endogâmicos BALB C |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2007 |
| dc.date.issued.fl_str_mv |
2007 |
| dc.date.accessioned.fl_str_mv |
2015-04-22T18:34:19Z |
| dc.date.available.fl_str_mv |
2015-04-22T18:34:19Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SILVA, E. M. et al. Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection. European Joural of Immunology, v. 37, n. 3, p. 738-746, 2007. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/10113 |
| dc.identifier.issn.none.fl_str_mv |
0014-2980 |
| dc.identifier.doi.none.fl_str_mv |
10.1002/eji.200636790 |
| identifier_str_mv |
SILVA, E. M. et al. Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection. European Joural of Immunology, v. 37, n. 3, p. 738-746, 2007. 0014-2980 10.1002/eji.200636790 |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Wiley-VCH Verlag GmbH & Co |
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Wiley-VCH Verlag GmbH & Co |
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