Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo

Detalhes bibliográficos
Autor(a) principal: Costa, Emmanoel V.
Data de Publicação: 2022
Outros Autores: Souza, César A. S. de, Galvão, Alexandre F. C., Silva, Valdenizia R., Santos, Luciano de S., Dias, Rosane B., Rocha, Clarissa A. Gurgel, Soares, Milena B. P., Silva, Felipe M. A. da, Koolen, Hector H. F., Bezerra, Daniel P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56141
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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spelling Costa, Emmanoel V.Souza, César A. S. deGalvão, Alexandre F. C.Silva, Valdenizia R.Santos, Luciano de S.Dias, Rosane B.Rocha, Clarissa A. GurgelSoares, Milena B. P.Silva, Felipe M. A. daKoolen, Hector H. F.Bezerra, Daniel P.2022-12-21T18:32:18Z2022-12-21T18:32:18Z2022Costa, Emmanoel V. et al. Duguetia pycnastera sandwith (Annonaceae) leaf essential oil inhibits HepG2 cell growth in vitro and in vivo. Molecules, v. 27, p. 1-14, 2022.1420-3049https://www.arca.fiocruz.br/handle/icict/5614110.3390/molecules 27175664Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil.Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil.Instituto SENAI de Inovação em Sistemas Avançados em Saúde. Centro Universitário SENAI/CIMATEC. Salvador, BA, Brasil.Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil.Universidade Estadual do Amazonas. Grupo de Pesquisa em Metabolômica e Espectrometria de Massas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Abstract: Duguetia pycnastera Sandwith (Annonaceae) is a tropical tree that can be found in the Guyanas, Bolivia, Venezuela, and Brazil. In Brazil, it is popularly known as “ata”, “envira”, “envirapreta”, and “envira-surucucu”. In the present work, we investigated the in vitro and in vivo HepG2 cell growth inhibition capacity of D. pycnastera leaf essential oil (EO). The chemical composition of the EO was determined by GC–MS and GC–FID analyses. The alamar blue assay was used to examine the in vitro cytotoxicity of EO in cancer cell lines and non-cancerous cells. In EO-treated HepG2 cells, DNA fragmentation was measured by flow cytometry. The in vivo antitumor activity of the EO was assessed in C.B-17 SCID mice xenografted with HepG2 cells treated with the EO at a dosage of 40 mg/kg. Chemical composition analysis displayed the sesquiterpenes -gurjunene (26.83%), bicyclogermacrene (24.90%), germacrene D (15.35%), and spathulenol (12.97%) as the main EO constituents. The EO exhibited cytotoxicity, with IC50 values ranging from 3.28 to 39.39 g/mL in the cancer cell lines SCC4 and CAL27, respectively. The cytotoxic activity of the EO in non-cancerous cells revealed IC50 values of 16.57, 21.28, and >50 g/mL for MRC-5, PBMC, and BJ cells, respectively. An increase of the fragmented DNA content was observed in EO-treated HepG2 cells. In vivo, EO displayed tumor mass inhibition activity by 47.76%. These findings imply that D. pycnastera leaf EO may have anti-liver cancer properties.engMDPIDuguetia pycnasteraÓleo essencialCélulas HepG2CitotóxicoantitumoralDuguetia pycnasteraEssential oilHepG2 cellsCytotoxicAntitumorÓleos VoláteisCélulas Hep G2CitotoxinasDuguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCosta , Emmanoel V. - Duguetia pycnastera Sandwith (Annonaceae) Leaf Essential Oil.pdfCosta , Emmanoel V. - Duguetia pycnastera Sandwith (Annonaceae) Leaf Essential Oil.pdfapplication/pdf4535091https://www.arca.fiocruz.br/bitstream/icict/56141/2/Costa%20%2c%20Emmanoel%20V.%20-%20Duguetia%20pycnastera%20Sandwith%20%28Annonaceae%29%20Leaf%20Essential%20Oil.pdf56b396873484827700d1a8334ed56a5dMD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
title Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
spellingShingle Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
Costa, Emmanoel V.
Duguetia pycnastera
Óleo essencial
Células HepG2
Citotóxico
antitumoral
Duguetia pycnastera
Essential oil
HepG2 cells
Cytotoxic
Antitumor
Óleos Voláteis
Células Hep G2
Citotoxinas
title_short Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
title_full Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
title_fullStr Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
title_full_unstemmed Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
title_sort Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
author Costa, Emmanoel V.
author_facet Costa, Emmanoel V.
Souza, César A. S. de
Galvão, Alexandre F. C.
Silva, Valdenizia R.
Santos, Luciano de S.
Dias, Rosane B.
Rocha, Clarissa A. Gurgel
Soares, Milena B. P.
Silva, Felipe M. A. da
Koolen, Hector H. F.
Bezerra, Daniel P.
author_role author
author2 Souza, César A. S. de
Galvão, Alexandre F. C.
Silva, Valdenizia R.
Santos, Luciano de S.
Dias, Rosane B.
Rocha, Clarissa A. Gurgel
Soares, Milena B. P.
Silva, Felipe M. A. da
Koolen, Hector H. F.
Bezerra, Daniel P.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Emmanoel V.
Souza, César A. S. de
Galvão, Alexandre F. C.
Silva, Valdenizia R.
Santos, Luciano de S.
Dias, Rosane B.
Rocha, Clarissa A. Gurgel
Soares, Milena B. P.
Silva, Felipe M. A. da
Koolen, Hector H. F.
Bezerra, Daniel P.
dc.subject.other.en_US.fl_str_mv Duguetia pycnastera
Óleo essencial
Células HepG2
Citotóxico
antitumoral
topic Duguetia pycnastera
Óleo essencial
Células HepG2
Citotóxico
antitumoral
Duguetia pycnastera
Essential oil
HepG2 cells
Cytotoxic
Antitumor
Óleos Voláteis
Células Hep G2
Citotoxinas
dc.subject.en.en_US.fl_str_mv Duguetia pycnastera
Essential oil
HepG2 cells
Cytotoxic
Antitumor
dc.subject.decs.en_US.fl_str_mv Óleos Voláteis
Células Hep G2
Citotoxinas
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-12-21T18:32:18Z
dc.date.available.fl_str_mv 2022-12-21T18:32:18Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Costa, Emmanoel V. et al. Duguetia pycnastera sandwith (Annonaceae) leaf essential oil inhibits HepG2 cell growth in vitro and in vivo. Molecules, v. 27, p. 1-14, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56141
dc.identifier.issn.en_US.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/molecules 27175664
identifier_str_mv Costa, Emmanoel V. et al. Duguetia pycnastera sandwith (Annonaceae) leaf essential oil inhibits HepG2 cell growth in vitro and in vivo. Molecules, v. 27, p. 1-14, 2022.
1420-3049
10.3390/molecules 27175664
url https://www.arca.fiocruz.br/handle/icict/56141
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56141/2/Costa%20%2c%20Emmanoel%20V.%20-%20Duguetia%20pycnastera%20Sandwith%20%28Annonaceae%29%20Leaf%20Essential%20Oil.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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