Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy

Detalhes bibliográficos
Autor(a) principal: Marins, Rachel Siqueira de Queiroz Simões
Data de Publicação: 2012
Outros Autores: Cassiano, Keila Mara, Pereira, Sílvia Regina Ferreira Gonçalves, Nogueira, Denise Monnerat, Carvalho, Eulógio Queiroz de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18816
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
id CRUZ_efeec52c69802a4b46775d657cc5577f
oai_identifier_str oai:www.arca.fiocruz.br:icict/18816
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Marins, Rachel Siqueira de Queiroz SimõesCassiano, Keila MaraPereira, Sílvia Regina Ferreira GonçalvesNogueira, Denise MonneratCarvalho, Eulógio Queiroz de2017-05-11T13:59:01Z2017-05-11T13:59:01Z2012MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012.2250-9941https://www.arca.fiocruz.br/handle/icict/18816engInternational Research JournalsPapilomavírus caninoCulturas de linfócitos do sangue periféricoCulturas celularesReação em Cadeia da Polimerasecanine papillomavirusperipheral blood lymphocytes culturestissue cell culturesPCRCanine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto de Matemática. Laboratório de Estatística. Campus Valonguinho, Niterói, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Campos dos Goytacazes, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Laboratório de Citogenética Animal. Campus Vital Brasil. Niterói, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Departamento de Anatomia Patológica e Morfológica. Laboratório de Saúde Animal. Campos dos Goytacazes, RJ, BrasilIn the present study, we reported about papillomavirus (PV) clinical infection and viral latent investigation in canines. Clinical and molecular analyses for detection of DNA PV infection, as well as histopathology examination and cytogenetic evaluation were carried out in clinical lesions canines. Blood sample from the both epithelial infection and the asymptomatic dogs had been collected for molecular analysis. Amplification of DNA samples with the L1 gene from generic primers were detected in skin biopsies and blood samples from canines healthy clinically or not. To investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and cutaneous warts lesions. The histological examinations of the skin biopsy specimens were diagnosed like adenoma, carcinoma and papilloma. In some cases, besides the surgical removal, autogenous vaccine derived from fresh warts of the same animal was indicated. The findings documented as mucosal and cutaneous tropism, which the PV viral infection not confined only epithelial sites, but also involved intracellular genome of host cell.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18816/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrachel_marins_etal_IOC_2012.pdfrachel_marins_etal_IOC_2012.pdfapplication/pdf382576https://www.arca.fiocruz.br/bitstream/icict/18816/2/rachel_marins_etal_IOC_2012.pdff90838574ea691479e6d0f8ad75c5e95MD52TEXTrachel_marins_etal_IOC_2012.pdf.txtrachel_marins_etal_IOC_2012.pdf.txtExtracted texttext/plain25181https://www.arca.fiocruz.br/bitstream/icict/18816/3/rachel_marins_etal_IOC_2012.pdf.txt8a6dac53e5b1054873495df8db96a953MD53icict/188162018-08-15 02:26:33.507oai:www.arca.fiocruz.br:icict/18816Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:26:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
title Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
spellingShingle Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
Marins, Rachel Siqueira de Queiroz Simões
Papilomavírus canino
Culturas de linfócitos do sangue periférico
Culturas celulares
Reação em Cadeia da Polimerase
canine papillomavirus
peripheral blood lymphocytes cultures
tissue cell cultures
PCR
title_short Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
title_full Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
title_fullStr Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
title_full_unstemmed Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
title_sort Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
author Marins, Rachel Siqueira de Queiroz Simões
author_facet Marins, Rachel Siqueira de Queiroz Simões
Cassiano, Keila Mara
Pereira, Sílvia Regina Ferreira Gonçalves
Nogueira, Denise Monnerat
Carvalho, Eulógio Queiroz de
author_role author
author2 Cassiano, Keila Mara
Pereira, Sílvia Regina Ferreira Gonçalves
Nogueira, Denise Monnerat
Carvalho, Eulógio Queiroz de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marins, Rachel Siqueira de Queiroz Simões
Cassiano, Keila Mara
Pereira, Sílvia Regina Ferreira Gonçalves
Nogueira, Denise Monnerat
Carvalho, Eulógio Queiroz de
dc.subject.other.pt_BR.fl_str_mv Papilomavírus canino
Culturas de linfócitos do sangue periférico
Culturas celulares
Reação em Cadeia da Polimerase
topic Papilomavírus canino
Culturas de linfócitos do sangue periférico
Culturas celulares
Reação em Cadeia da Polimerase
canine papillomavirus
peripheral blood lymphocytes cultures
tissue cell cultures
PCR
dc.subject.en.pt_BR.fl_str_mv canine papillomavirus
peripheral blood lymphocytes cultures
tissue cell cultures
PCR
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-11T13:59:01Z
dc.date.available.fl_str_mv 2017-05-11T13:59:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18816
dc.identifier.issn.pt_BR.fl_str_mv 2250-9941
identifier_str_mv MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012.
2250-9941
url https://www.arca.fiocruz.br/handle/icict/18816
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv International Research Journals
publisher.none.fl_str_mv International Research Journals
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18816/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18816/2/rachel_marins_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/18816/3/rachel_marins_etal_IOC_2012.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
f90838574ea691479e6d0f8ad75c5e95
8a6dac53e5b1054873495df8db96a953
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009290026287104