Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18816 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. |
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Marins, Rachel Siqueira de Queiroz SimõesCassiano, Keila MaraPereira, Sílvia Regina Ferreira GonçalvesNogueira, Denise MonneratCarvalho, Eulógio Queiroz de2017-05-11T13:59:01Z2017-05-11T13:59:01Z2012MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012.2250-9941https://www.arca.fiocruz.br/handle/icict/18816engInternational Research JournalsPapilomavírus caninoCulturas de linfócitos do sangue periféricoCulturas celularesReação em Cadeia da Polimerasecanine papillomavirusperipheral blood lymphocytes culturestissue cell culturesPCRCanine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto de Matemática. Laboratório de Estatística. Campus Valonguinho, Niterói, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Campos dos Goytacazes, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Laboratório de Citogenética Animal. Campus Vital Brasil. Niterói, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Agricultura e Tecnologia Agrícola. Departamento de Anatomia Patológica e Morfológica. Laboratório de Saúde Animal. Campos dos Goytacazes, RJ, BrasilIn the present study, we reported about papillomavirus (PV) clinical infection and viral latent investigation in canines. Clinical and molecular analyses for detection of DNA PV infection, as well as histopathology examination and cytogenetic evaluation were carried out in clinical lesions canines. Blood sample from the both epithelial infection and the asymptomatic dogs had been collected for molecular analysis. Amplification of DNA samples with the L1 gene from generic primers were detected in skin biopsies and blood samples from canines healthy clinically or not. To investigate the chromosomal fragility, we researched by cytogenetic technique in the peripheral blood lymphocytes cultures and in the tumors cells cultures from canines presenting oral and cutaneous warts lesions. The histological examinations of the skin biopsy specimens were diagnosed like adenoma, carcinoma and papilloma. In some cases, besides the surgical removal, autogenous vaccine derived from fresh warts of the same animal was indicated. The findings documented as mucosal and cutaneous tropism, which the PV viral infection not confined only epithelial sites, but also involved intracellular genome of host cell.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18816/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrachel_marins_etal_IOC_2012.pdfrachel_marins_etal_IOC_2012.pdfapplication/pdf382576https://www.arca.fiocruz.br/bitstream/icict/18816/2/rachel_marins_etal_IOC_2012.pdff90838574ea691479e6d0f8ad75c5e95MD52TEXTrachel_marins_etal_IOC_2012.pdf.txtrachel_marins_etal_IOC_2012.pdf.txtExtracted texttext/plain25181https://www.arca.fiocruz.br/bitstream/icict/18816/3/rachel_marins_etal_IOC_2012.pdf.txt8a6dac53e5b1054873495df8db96a953MD53icict/188162018-08-15 02:26:33.507oai:www.arca.fiocruz.br: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ório 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dc.title.pt_BR.fl_str_mv |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
title |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
spellingShingle |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy Marins, Rachel Siqueira de Queiroz Simões Papilomavírus canino Culturas de linfócitos do sangue periférico Culturas celulares Reação em Cadeia da Polimerase canine papillomavirus peripheral blood lymphocytes cultures tissue cell cultures PCR |
title_short |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
title_full |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
title_fullStr |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
title_full_unstemmed |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
title_sort |
Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy |
author |
Marins, Rachel Siqueira de Queiroz Simões |
author_facet |
Marins, Rachel Siqueira de Queiroz Simões Cassiano, Keila Mara Pereira, Sílvia Regina Ferreira Gonçalves Nogueira, Denise Monnerat Carvalho, Eulógio Queiroz de |
author_role |
author |
author2 |
Cassiano, Keila Mara Pereira, Sílvia Regina Ferreira Gonçalves Nogueira, Denise Monnerat Carvalho, Eulógio Queiroz de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Marins, Rachel Siqueira de Queiroz Simões Cassiano, Keila Mara Pereira, Sílvia Regina Ferreira Gonçalves Nogueira, Denise Monnerat Carvalho, Eulógio Queiroz de |
dc.subject.other.pt_BR.fl_str_mv |
Papilomavírus canino Culturas de linfócitos do sangue periférico Culturas celulares Reação em Cadeia da Polimerase |
topic |
Papilomavírus canino Culturas de linfócitos do sangue periférico Culturas celulares Reação em Cadeia da Polimerase canine papillomavirus peripheral blood lymphocytes cultures tissue cell cultures PCR |
dc.subject.en.pt_BR.fl_str_mv |
canine papillomavirus peripheral blood lymphocytes cultures tissue cell cultures PCR |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2017-05-11T13:59:01Z |
dc.date.available.fl_str_mv |
2017-05-11T13:59:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/18816 |
dc.identifier.issn.pt_BR.fl_str_mv |
2250-9941 |
identifier_str_mv |
MARINS, Rachel Siqueira de Queiroz Simões; et al. Canine latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures from lesions biopsy. International Research Journal of Biochemistry and Bioinformatics, v. 2, n.3, p.62-68, Mar. 2012. 2250-9941 |
url |
https://www.arca.fiocruz.br/handle/icict/18816 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
International Research Journals |
publisher.none.fl_str_mv |
International Research Journals |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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