Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12126 |
Resumo: | 2016-02-29 |
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Lima, Estela de OliveiraMacedo, Cristiana Santos deEsteves, Cibele ZanardiOliveira, Diogo Noin dePessolani, Maria Cristina VidalNery, José Augusto da CostaSarno, Euzenir NunesCatharino, Rodrigo Ramos2015-11-04T14:41:43Z2016-03-01T07:30:08Z2015LIMA, Estela de Oliveira; et al. Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry. Analytical Chemistry, v.87, p.3585−3592, 2015.0003-2700https://www.arca.fiocruz.br/handle/icict/1212610.1021/acs.analchem.5b00097engAmerican Chemical SocietyMetodologia de diagnóstico potencial para HanseníaseLeprosyHigh-Resolution Electrospray Mass Spectrometry (ESIHRMS)Skin Imprinting in Silica PlatesPotential Diagnostic Methodology for LeprosyHanseníaseEspectrometria de MassasPlacas de sílicaSkin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-02-29Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnólogico em Saúde (CDTS). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Ambulatório Souza Araújo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.Leprosy is a chronic infectious disease caused by Mycobacterium leprae, which primarily infects macrophages and Schwann cells, affecting skin and peripheral nerves. Clinically, the most common form of identification is through the observation of anesthetic lesions on skin; however, up to 30% of infected patients may not present this clinical manifestation. Currently, the gold standard diagnostic test for leprosy is based on skin lesion biopsy, which is invasive and presents low sensibility for suspect cases. Therefore, the development of a fast, sensible and noninvasive method that identifies infected patients would be helpful for assertive diagnosis. The aim of this work was to identify lipid markers in leprosy patients directly from skin imprints, using a mass spectrometric analytical strategy. For skin imprint samples, a 1 cm2 silica plate was gently pressed against the skin of patients or healthy volunteers. Imprinted silica lipids were extracted and submitted to direct-infusion electrospray ionization high-resolution mass spectrometry (ESI-HRMS). All samples were differentiated using a lipidomics-based data workup employing multivariate data analysis, which helped electing different lipid markers, for example, mycobacterial mycolic acids, inflammatory and apoptotic molecules were identified as leprosy patients’ markers. Otherwise, phospholipids and gangliosides were pointed as healthy volunteers’ skin lipid markers, according to normal skin composition. Results indicate that silica plate skin imprinting associated with ESI-HRMS is a promising fast and sensible leprosy diagnostic method. With a prompt leprosy diagnosis, an early and effective treatment could be feasible and thus the chain of leprosy transmission could be abbreviated.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12126/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALcristiana_macedo_etal_IOC_2015.pdfcristiana_macedo_etal_IOC_2015.pdfapplication/pdf1860399https://www.arca.fiocruz.br/bitstream/icict/12126/2/cristiana_macedo_etal_IOC_2015.pdf20eda8cfc73e425563b47adde66d221bMD52TEXTcristiana_macedo_etal_IOC_2015.pdf.txtcristiana_macedo_etal_IOC_2015.pdf.txtExtracted texttext/plain39296https://www.arca.fiocruz.br/bitstream/icict/12126/3/cristiana_macedo_etal_IOC_2015.pdf.txt036add193eec007caa6a63ceff38f105MD53icict/121262023-09-04 11:10:38.547oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:10:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| title |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| spellingShingle |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry Lima, Estela de Oliveira Metodologia de diagnóstico potencial para Hanseníase Leprosy High-Resolution Electrospray Mass Spectrometry (ESIHRMS) Skin Imprinting in Silica Plates Potential Diagnostic Methodology for Leprosy Hanseníase Espectrometria de Massas Placas de sílica |
| title_short |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| title_full |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| title_fullStr |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| title_full_unstemmed |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| title_sort |
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry |
| author |
Lima, Estela de Oliveira |
| author_facet |
Lima, Estela de Oliveira Macedo, Cristiana Santos de Esteves, Cibele Zanardi Oliveira, Diogo Noin de Pessolani, Maria Cristina Vidal Nery, José Augusto da Costa Sarno, Euzenir Nunes Catharino, Rodrigo Ramos |
| author_role |
author |
| author2 |
Macedo, Cristiana Santos de Esteves, Cibele Zanardi Oliveira, Diogo Noin de Pessolani, Maria Cristina Vidal Nery, José Augusto da Costa Sarno, Euzenir Nunes Catharino, Rodrigo Ramos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Estela de Oliveira Macedo, Cristiana Santos de Esteves, Cibele Zanardi Oliveira, Diogo Noin de Pessolani, Maria Cristina Vidal Nery, José Augusto da Costa Sarno, Euzenir Nunes Catharino, Rodrigo Ramos |
| dc.subject.other.pt_BR.fl_str_mv |
Metodologia de diagnóstico potencial para Hanseníase |
| topic |
Metodologia de diagnóstico potencial para Hanseníase Leprosy High-Resolution Electrospray Mass Spectrometry (ESIHRMS) Skin Imprinting in Silica Plates Potential Diagnostic Methodology for Leprosy Hanseníase Espectrometria de Massas Placas de sílica |
| dc.subject.en.pt_BR.fl_str_mv |
Leprosy High-Resolution Electrospray Mass Spectrometry (ESIHRMS) Skin Imprinting in Silica Plates Potential Diagnostic Methodology for Leprosy |
| dc.subject.decs.pt_BR.fl_str_mv |
Hanseníase Espectrometria de Massas Placas de sílica |
| description |
2016-02-29 |
| publishDate |
2015 |
| dc.date.accessioned.fl_str_mv |
2015-11-04T14:41:43Z |
| dc.date.issued.fl_str_mv |
2015 |
| dc.date.available.fl_str_mv |
2016-03-01T07:30:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
LIMA, Estela de Oliveira; et al. Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry. Analytical Chemistry, v.87, p.3585−3592, 2015. |
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https://www.arca.fiocruz.br/handle/icict/12126 |
| dc.identifier.issn.none.fl_str_mv |
0003-2700 |
| dc.identifier.doi.none.fl_str_mv |
10.1021/acs.analchem.5b00097 |
| identifier_str_mv |
LIMA, Estela de Oliveira; et al. Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry. Analytical Chemistry, v.87, p.3585−3592, 2015. 0003-2700 10.1021/acs.analchem.5b00097 |
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https://www.arca.fiocruz.br/handle/icict/12126 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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American Chemical Society |
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American Chemical Society |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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