The influence of iron on the proteomic profile of Chromobacterium violaceum

Detalhes bibliográficos
Autor(a) principal: Lima, Daniel C.
Data de Publicação: 2014
Outros Autores: Duarte, Fábio T., Medeiros, Sivia Regina Batistuzzo de, Lima, Diogo Borges, Carvalho, Paulo Costa, Bonatto, Diego, Medeiros, Silvia R. Batistuzzo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16109
Resumo: CNPq, INEspaço, Fiocruz – PDTIS, FAPERJ, CAPES
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spelling Lima, Daniel C.Duarte, Fábio T.Medeiros, Sivia Regina Batistuzzo deLima, Diogo BorgesCarvalho, Paulo CostaBonatto, DiegoMedeiros, Silvia R. Batistuzzo de2016-10-05T19:32:19Z2016-10-05T19:32:19Z2014LIMA, Daniel C. et al. The influence of iron on the proteomic profile of Chromobacterium violaceum. BMC Microbiology, v. 14, n. 267, p. 1-12, 2014.1471-2180https://www.arca.fiocruz.br/handle/icict/1610910.1186/s12866-014-0267-6CNPq, INEspaço, Fiocruz – PDTIS, FAPERJ, CAPESUniversidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular. Natal, RN, Brasil.Instituto Federal de Educação, Ciência e Tecnologia. São Gonçalo do Amarante, RN, Brasil.Universidade Federal do Vale do São Francisco. Petrolina, PE, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Biologia Molecular. Natal, RN, Brasil.Background Chromobacterium violaceum is a bacterium commonly found in tropical and subtropical regions and is associated with important pharmacological and industrial attributes such as producing substances with therapeutic properties and synthesizing biodegradable polymers. Its genome was sequenced, however, approximately 40% of its genes still remain with unknown functions. Although C. violaceum is known by its versatile capacity of living in a wide range of environments, little is known on how it achieves such success. Here, we investigated the proteomic profile of C. violaceum cultivated in the absence and presence of high iron concentration, describing some proteins of unknown function that might play an important role in iron homeostasis, amongst others. Results Briefly, C. violaceum was cultivated in the absence and in the presence of 9mM of iron during four hours. Total proteins were identified by LC-MS and through the PatternLab pipeline. Our proteomic analysis indicates major changes in the energetic metabolism, and alterations in the synthesis of key transport and stress proteins. In addition, it may suggest the presence of a yet unidentified operon that could be related to oxidative stress, together with a set of other proteins with unknown function. The protein-protein interaction network also pinpointed the importance of energetic metabolism proteins to the acclimatation of C. violaceum in high concentration of iron. Conclusions This is the first proteomic analysis of the opportunistic pathogen C. violaceum in the presence of high iron concentration. Our data allowed us to identify a yet undescribed operon that might have a role in oxidative stress defense. Our work provides new data that will contribute to understand how this bacterium achieve its capacity of surviving in harsh conditions as well as to open a way to explore the yet little availed biotechnological characteristics of this bacterium with the further exploring of the proteins of unknown function that we showed to be up-regulated in high iron concentration.engTim SandsThe influence of iron on the proteomic profile of Chromobacterium violaceuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEnergetic metabolismCoordinated adaptationSodChromobacterium violaceumSuperoxide DismutaseMetabolismo EnergéticoAdaptaçãoChromobacteriumSuperóxido Dismutaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16109/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALThe influence of iron on the proteomic profile of Chromobacterium violaceum.pdfThe influence of iron on the proteomic profile of Chromobacterium violaceum.pdfapplication/pdf1071999https://www.arca.fiocruz.br/bitstream/icict/16109/2/The%20influence%20of%20iron%20on%20the%20proteomic%20profile%20of%20Chromobacterium%20violaceum.pdf896e5f9a81cef2f8a30af59bad79fbe3MD52TEXTThe influence of iron on the proteomic profile of Chromobacterium violaceum.pdf.txtThe influence of iron on the proteomic profile of Chromobacterium violaceum.pdf.txtExtracted texttext/plain52641https://www.arca.fiocruz.br/bitstream/icict/16109/3/The%20influence%20of%20iron%20on%20the%20proteomic%20profile%20of%20Chromobacterium%20violaceum.pdf.txtd57a600258a9a3fa7b66b8a7eb6a1351MD53icict/161092019-11-23 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dc.title.pt_BR.fl_str_mv The influence of iron on the proteomic profile of Chromobacterium violaceum
title The influence of iron on the proteomic profile of Chromobacterium violaceum
spellingShingle The influence of iron on the proteomic profile of Chromobacterium violaceum
Lima, Daniel C.
Energetic metabolism
Coordinated adaptation
Sod
Chromobacterium violaceum
Superoxide Dismutase
Metabolismo Energético
Adaptação
Chromobacterium
Superóxido Dismutase
title_short The influence of iron on the proteomic profile of Chromobacterium violaceum
title_full The influence of iron on the proteomic profile of Chromobacterium violaceum
title_fullStr The influence of iron on the proteomic profile of Chromobacterium violaceum
title_full_unstemmed The influence of iron on the proteomic profile of Chromobacterium violaceum
title_sort The influence of iron on the proteomic profile of Chromobacterium violaceum
author Lima, Daniel C.
author_facet Lima, Daniel C.
Duarte, Fábio T.
Medeiros, Sivia Regina Batistuzzo de
Lima, Diogo Borges
Carvalho, Paulo Costa
Bonatto, Diego
Medeiros, Silvia R. Batistuzzo de
author_role author
author2 Duarte, Fábio T.
Medeiros, Sivia Regina Batistuzzo de
Lima, Diogo Borges
Carvalho, Paulo Costa
Bonatto, Diego
Medeiros, Silvia R. Batistuzzo de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Daniel C.
Duarte, Fábio T.
Medeiros, Sivia Regina Batistuzzo de
Lima, Diogo Borges
Carvalho, Paulo Costa
Bonatto, Diego
Medeiros, Silvia R. Batistuzzo de
dc.subject.en.pt_BR.fl_str_mv Energetic metabolism
Coordinated adaptation
Sod
Chromobacterium violaceum
topic Energetic metabolism
Coordinated adaptation
Sod
Chromobacterium violaceum
Superoxide Dismutase
Metabolismo Energético
Adaptação
Chromobacterium
Superóxido Dismutase
dc.subject.en.En.fl_str_mv Superoxide Dismutase
dc.subject.decs.pt_BR.fl_str_mv Metabolismo Energético
Adaptação
Chromobacterium
Superóxido Dismutase
description CNPq, INEspaço, Fiocruz – PDTIS, FAPERJ, CAPES
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-10-05T19:32:19Z
dc.date.available.fl_str_mv 2016-10-05T19:32:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Daniel C. et al. The influence of iron on the proteomic profile of Chromobacterium violaceum. BMC Microbiology, v. 14, n. 267, p. 1-12, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16109
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s12866-014-0267-6
identifier_str_mv LIMA, Daniel C. et al. The influence of iron on the proteomic profile of Chromobacterium violaceum. BMC Microbiology, v. 14, n. 267, p. 1-12, 2014.
1471-2180
10.1186/s12866-014-0267-6
url https://www.arca.fiocruz.br/handle/icict/16109
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Tim Sands
publisher.none.fl_str_mv Tim Sands
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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