Structural basis for glucose tolerance in GH1 b-glucosidases

Detalhes bibliográficos
Autor(a) principal: Giuseppe, Priscila Oliveira de
Data de Publicação: 2014
Outros Autores: Souza, Tatiana de Arruda Campos Brasil de, Souza, Flavio Henrique Moreira, Zanphorlin, Leticia Maria, Machado, Carla Botelho, Ward, Richard John, Jorge, João Atilio, Furriel, Rosa dos Prazeres Melo, Murakami, Mario Tyago
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8961
Resumo: 2015-12-31
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spelling Giuseppe, Priscila Oliveira deSouza, Tatiana de Arruda Campos Brasil deSouza, Flavio Henrique MoreiraZanphorlin, Leticia MariaMachado, Carla BotelhoWard, Richard JohnJorge, João AtilioFurriel, Rosa dos Prazeres MeloMurakami, Mario Tyago2014-11-25T17:03:08Z2016-01-01T06:30:04Z2014GIUSEPPE, Priscila Oliveira de et al. Structural basis for glucose tolerance in GH1 b-glucosidases. International Union of Crystallography, Chester GB, v. D70, p. 1631-1639, 2014.1399-0047https://www.arca.fiocruz.br/handle/icict/8961engStructural basis for glucose tolerance in GH1 b-glucosidasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2015-12-31Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras. Departamento de Química. Ribeirão Preto, SP, Brasil.Universidade Estadual de Campinas. Instituto de Química. Departamento de Química Orgânica. Campinas, SP, Brasil.Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras. Departamento de Química. Ribeirão Preto, SP, Brasil / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de de Ciência e Tecnologia do Bioetanol. Campinas, SP, Brasil.Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras. Departamento de Química. Ribeirão Preto, SP, Brasil / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de de Ciência e Tecnologia do Bioetanol. Campinas, SP, Brasil.Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras. Departamento de Biologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras. Departamento de Química. Ribeirão Preto, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. Campinas, SP, Brasil.Product inhibition of β-glucosidases (BGs) by glucose is considered to be a limiting step in enzymatic technologies for plant-biomass saccharification. Remarkably, some β-glucosidases belonging to the GH1 family exhibit unusual properties, being tolerant to, or even stimulated by, high glucose concentrations. However, the structural basis for the glucose tolerance and stimulation of BGs is still elusive. To address this issue, the first crystal structure of a fungal β-glucosidase stimulated by glucose was solved in native and glucose-complexed forms, revealing that the shape and electrostatic properties of the entrance to the active site, including the +2 subsite, determine glucose tolerance. The aromatic Trp168 and the aliphatic Leu173 are conserved in glucose-tolerant GH1 enzymes and contribute to relieving enzyme inhibition by imposing constraints at the +2 subsite that limit the access of glucose to the -1 subsite. The GH1 family β-glucosidases are tenfold to 1000-fold more glucose tolerant than GH3 BGs, and comparative structural analysis shows a clear correlation between active-site accessibility and glucose tolerance. The active site of GH1 BGs is located in a deep and narrow cavity, which is in contrast to the shallow pocket in the GH3 family BGs. These findings shed light on the molecular basis for glucose tolerance and indicate that GH1 BGs are more suitable than GH3 BGs for biotechnological applications involving plant cell-wall saccharification.BiochemistryCellulasesBiofuelsCelulasasBiocombustiblesBioquímicaCelulasesBiocombustíveisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8961/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALJ. Biol. Chem.-1997-Nare-13883-91.pdfJ. Biol. Chem.-1997-Nare-13883-91.pdfapplication/pdf1511856https://www.arca.fiocruz.br/bitstream/icict/8961/2/J.%20Biol.%20Chem.-1997-Nare-13883-91.pdf1819e3063bfd29935a7afcb91b0aa294MD52TEXTJ. Biol. Chem.-1997-Nare-13883-91.pdf.txtJ. Biol. Chem.-1997-Nare-13883-91.pdf.txtExtracted texttext/plain37538https://www.arca.fiocruz.br/bitstream/icict/8961/3/J.%20Biol.%20Chem.-1997-Nare-13883-91.pdf.txte32a3697bf00f264c94b4c5e2a32a72dMD53icict/89612021-02-10 19:38:00.04oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-02-10T22:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Structural basis for glucose tolerance in GH1 b-glucosidases
title Structural basis for glucose tolerance in GH1 b-glucosidases
spellingShingle Structural basis for glucose tolerance in GH1 b-glucosidases
Giuseppe, Priscila Oliveira de
Biochemistry
Cellulases
Biofuels
Celulasas
Biocombustibles
Bioquímica
Celulases
Biocombustíveis
title_short Structural basis for glucose tolerance in GH1 b-glucosidases
title_full Structural basis for glucose tolerance in GH1 b-glucosidases
title_fullStr Structural basis for glucose tolerance in GH1 b-glucosidases
title_full_unstemmed Structural basis for glucose tolerance in GH1 b-glucosidases
title_sort Structural basis for glucose tolerance in GH1 b-glucosidases
author Giuseppe, Priscila Oliveira de
author_facet Giuseppe, Priscila Oliveira de
Souza, Tatiana de Arruda Campos Brasil de
Souza, Flavio Henrique Moreira
Zanphorlin, Leticia Maria
Machado, Carla Botelho
Ward, Richard John
Jorge, João Atilio
Furriel, Rosa dos Prazeres Melo
Murakami, Mario Tyago
author_role author
author2 Souza, Tatiana de Arruda Campos Brasil de
Souza, Flavio Henrique Moreira
Zanphorlin, Leticia Maria
Machado, Carla Botelho
Ward, Richard John
Jorge, João Atilio
Furriel, Rosa dos Prazeres Melo
Murakami, Mario Tyago
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Giuseppe, Priscila Oliveira de
Souza, Tatiana de Arruda Campos Brasil de
Souza, Flavio Henrique Moreira
Zanphorlin, Leticia Maria
Machado, Carla Botelho
Ward, Richard John
Jorge, João Atilio
Furriel, Rosa dos Prazeres Melo
Murakami, Mario Tyago
dc.subject.en.En.fl_str_mv Biochemistry
Cellulases
Biofuels
topic Biochemistry
Cellulases
Biofuels
Celulasas
Biocombustibles
Bioquímica
Celulases
Biocombustíveis
dc.subject.es.Es.fl_str_mv Celulasas
Biocombustibles
dc.subject.decs.pt_BR.fl_str_mv Bioquímica
Celulases
Biocombustíveis
description 2015-12-31
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-11-25T17:03:08Z
dc.date.issued.fl_str_mv 2014
dc.date.available.fl_str_mv 2016-01-01T06:30:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GIUSEPPE, Priscila Oliveira de et al. Structural basis for glucose tolerance in GH1 b-glucosidases. International Union of Crystallography, Chester GB, v. D70, p. 1631-1639, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8961
dc.identifier.issn.none.fl_str_mv 1399-0047
identifier_str_mv GIUSEPPE, Priscila Oliveira de et al. Structural basis for glucose tolerance in GH1 b-glucosidases. International Union of Crystallography, Chester GB, v. D70, p. 1631-1639, 2014.
1399-0047
url https://www.arca.fiocruz.br/handle/icict/8961
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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