A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology

Detalhes bibliográficos
Autor(a) principal: Ferrão, Patrícia M.
Data de Publicação: 2012
Outros Autores: Oliveira, Fabiane L. de, Degrave, Wim M., Araujo-Jorge, Tania C., Lima, Leila Mendonça, Waghabi, Mariana C.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18381
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Ferrão, Patrícia M.Oliveira, Fabiane L. deDegrave, Wim M.Araujo-Jorge, Tania C.Lima, Leila MendonçaWaghabi, Mariana C.2017-04-11T16:08:08Z2017-04-11T16:08:08Z2012FERRÃO, Patrícia M. et al. A Phosphoproteomic Approach towards the Understanding of the Role of TGF-b in Trypanosoma cruzi Biology. Plos One, v.7, n.6, e38736, 16p, June 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1838110.1371/journal.pone.0038736porPublic Library of ScienceTrypanosoma cruziDoença de ChagasAbordagem FosfoproteómicaFator de crescimento de transformação beta (TGF-b)Trypanosoma cruziChagas diseasePhosphoproteomic ApproachTGF-bA phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Transforming growth factor beta (TGF-β) plays a pivotal role in Chagas disease, not only in the development of chagasic cardiomyopathy, but also in many stages of the T. cruzi life cycle and survival in the host cell environment. The intracellular signaling pathways utilized by T. cruzi to regulate these mechanisms remain unknown. To identify parasite proteins involved in the TGF-β response, we utilized a combined approach of two-dimensional gel electrophoresis (2DE) analysis and mass spectrometry (MS) protein identification. Signaling via TGF-β is dependent on events of phosphorylation, which is one of the most relevant and ubiquitous post-translational modifications for the regulation of gene expression, and especially in trypanosomatids, since they lack several transcriptional control mechanisms. Here we show a kinetic view of T. cruzi epimastigotes (Y strain) incubated with TGF-β for 1, 5, 30 and 60 minutes, which promoted a remodeling of the parasite phosphorylation network and protein expression pattern. The altered molecules are involved in a variety of cellular processes, such as proteolysis, metabolism, heat shock response, cytoskeleton arrangement, oxidative stress regulation, translation and signal transduction. A total of 75 protein spots were up- or down-regulated more than twofold after TGF-β treatment, and from these, 42 were identified by mass spectrometry, including cruzipain-the major T. cruzi papain-like cysteine proteinase that plays an important role in invasion and participates in the escape mechanisms used by the parasite to evade the host immune system. In our study, we observed that TGF-β addition favored epimastigote proliferation, corroborating 2DE data in which proteins previously described to be involved in this process were positively stimulated by TGF-β.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18381/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpatricia_ferrao_etal_IOC_2012.pdfpatricia_ferrao_etal_IOC_2012.pdfapplication/pdf894361https://www.arca.fiocruz.br/bitstream/icict/18381/2/patricia_ferrao_etal_IOC_2012.pdff1c583394102575fa8b3f159fb9b5204MD52TEXTpatricia_ferrao_etal_IOC_2012.pdf.txtpatricia_ferrao_etal_IOC_2012.pdf.txtExtracted texttext/plain63164https://www.arca.fiocruz.br/bitstream/icict/18381/3/patricia_ferrao_etal_IOC_2012.pdf.txt4d4c6b529395533a3581606c91435b72MD53icict/183812022-06-24 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dc.title.pt_BR.fl_str_mv A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
title A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
spellingShingle A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
Ferrão, Patrícia M.
Trypanosoma cruzi
Doença de Chagas
Abordagem Fosfoproteómica
Fator de crescimento de transformação beta (TGF-b)
Trypanosoma cruzi
Chagas disease
Phosphoproteomic Approach
TGF-b
title_short A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
title_full A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
title_fullStr A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
title_full_unstemmed A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
title_sort A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology
author Ferrão, Patrícia M.
author_facet Ferrão, Patrícia M.
Oliveira, Fabiane L. de
Degrave, Wim M.
Araujo-Jorge, Tania C.
Lima, Leila Mendonça
Waghabi, Mariana C.
author_role author
author2 Oliveira, Fabiane L. de
Degrave, Wim M.
Araujo-Jorge, Tania C.
Lima, Leila Mendonça
Waghabi, Mariana C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferrão, Patrícia M.
Oliveira, Fabiane L. de
Degrave, Wim M.
Araujo-Jorge, Tania C.
Lima, Leila Mendonça
Waghabi, Mariana C.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Abordagem Fosfoproteómica
Fator de crescimento de transformação beta (TGF-b)
topic Trypanosoma cruzi
Doença de Chagas
Abordagem Fosfoproteómica
Fator de crescimento de transformação beta (TGF-b)
Trypanosoma cruzi
Chagas disease
Phosphoproteomic Approach
TGF-b
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas disease
Phosphoproteomic Approach
TGF-b
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-04-11T16:08:08Z
dc.date.available.fl_str_mv 2017-04-11T16:08:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRÃO, Patrícia M. et al. A Phosphoproteomic Approach towards the Understanding of the Role of TGF-b in Trypanosoma cruzi Biology. Plos One, v.7, n.6, e38736, 16p, June 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18381
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0038736
identifier_str_mv FERRÃO, Patrícia M. et al. A Phosphoproteomic Approach towards the Understanding of the Role of TGF-b in Trypanosoma cruzi Biology. Plos One, v.7, n.6, e38736, 16p, June 2012.
1932-6203
10.1371/journal.pone.0038736
url https://www.arca.fiocruz.br/handle/icict/18381
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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