Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15503 |
Resumo: | Fundação Oswaldo Cruz. Conselho Nacional de Desenvolvimento e Tecnológico. Fundação Araucária |
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Baptista, Carlos GustavoRodrigues, Elizabeth CristinaMorking, Patricia AlvesKlinke, AmandaZardo, Maria LuizaSoares, Maurilio JoséAguiar, Alessandra Melo deGoldenberg, SamuelRamos, Augusto Savio Peixoto2016-08-29T17:26:01Z2016-08-29T17:26:01Z2015BAPTISTA, Carlos Augusto et al. Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi. BMC Microbiology, v.15, n. 269, p. 1-10, 2015.1471-2180https://www.arca.fiocruz.br/handle/icict/15503Fundação Oswaldo Cruz. Conselho Nacional de Desenvolvimento e Tecnológico. Fundação AraucáriaUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Background Nucleotide sugar transporters (NSTs) play an essential role in translocating nucleotide sugars into the lumen of the endoplasmic reticulum and Golgi apparatus to be used as substrates in glycosylation reactions. This intracellular transport is an essential step in the biosynthesis of glycoconjugates. Results We have identified a family of 11 putative NSTs in Trypanosoma cruzi, the etiological agent of Chagas’ disease. A UDP-N-acetylglucosamine transporter, TcNST1, was identified by a yeast complementation approach. Based on a phylogenetic analysis four candidate genes were selected and used for complementation assays in a Kluyveromyces lactis mutant strain. The transporter is likely expressed in all stages of the parasite life cycle and during differentiation of epimastigotes to infective metacyclics. Immunofluorescence analyses of a GFP-TcNST1 fusion protein indicate that the transporter is localized to the Golgi apparatus. As many NSTs are multisubstrate transporters, we also tested the capacity of TcNST1 to transport GDP-Man. Conclusions We have identified a UDP-N-acetylglucosamine transporter in T. cruzi, which is specifically localized to the Golgi apparatus and seems to be expressed, at the mRNA level, throughout the parasite life cycle. Functional studies of TcNST1 will be important to unravel the role of NSTs and specific glycoconjugates in T. cruzi survival and infectivity.porIdentification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGlycoconjugatesNucleotide sugarTransporterTrypanosomatidsGlicoconjugadosAçúcarProteínas Vesiculares de Transporte de Aminas Biogênicasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15503/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIdentification of a Golgi-localized UDP-N-acetylglucosamine.pdfIdentification of a Golgi-localized UDP-N-acetylglucosamine.pdfapplication/pdf1462528https://www.arca.fiocruz.br/bitstream/icict/15503/2/Identification%20of%20a%20Golgi-localized%20UDP-N-acetylglucosamine.pdf4ee82b612b65e97fc2bf07e38093715eMD52TEXTIdentification of a Golgi-localized UDP-N-acetylglucosamine.pdf.txtIdentification of a Golgi-localized UDP-N-acetylglucosamine.pdf.txtExtracted texttext/plain42327https://www.arca.fiocruz.br/bitstream/icict/15503/3/Identification%20of%20a%20Golgi-localized%20UDP-N-acetylglucosamine.pdf.txt4355ed2f2cfbc9e15760434881f34872MD53icict/155032019-11-01 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| dc.title.pt_BR.fl_str_mv |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| title |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| spellingShingle |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi Baptista, Carlos Gustavo Glycoconjugates Nucleotide sugar Transporter Trypanosomatids Glicoconjugados Açúcar Proteínas Vesiculares de Transporte de Aminas Biogênicas |
| title_short |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| title_full |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| title_fullStr |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| title_full_unstemmed |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| title_sort |
Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi |
| author |
Baptista, Carlos Gustavo |
| author_facet |
Baptista, Carlos Gustavo Rodrigues, Elizabeth Cristina Morking, Patricia Alves Klinke, Amanda Zardo, Maria Luiza Soares, Maurilio José Aguiar, Alessandra Melo de Goldenberg, Samuel Ramos, Augusto Savio Peixoto |
| author_role |
author |
| author2 |
Rodrigues, Elizabeth Cristina Morking, Patricia Alves Klinke, Amanda Zardo, Maria Luiza Soares, Maurilio José Aguiar, Alessandra Melo de Goldenberg, Samuel Ramos, Augusto Savio Peixoto |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Baptista, Carlos Gustavo Rodrigues, Elizabeth Cristina Morking, Patricia Alves Klinke, Amanda Zardo, Maria Luiza Soares, Maurilio José Aguiar, Alessandra Melo de Goldenberg, Samuel Ramos, Augusto Savio Peixoto |
| dc.subject.en.pt_BR.fl_str_mv |
Glycoconjugates Nucleotide sugar Transporter Trypanosomatids |
| topic |
Glycoconjugates Nucleotide sugar Transporter Trypanosomatids Glicoconjugados Açúcar Proteínas Vesiculares de Transporte de Aminas Biogênicas |
| dc.subject.decs.pt_BR.fl_str_mv |
Glicoconjugados Açúcar Proteínas Vesiculares de Transporte de Aminas Biogênicas |
| description |
Fundação Oswaldo Cruz. Conselho Nacional de Desenvolvimento e Tecnológico. Fundação Araucária |
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2015 |
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2015 |
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2016-08-29T17:26:01Z |
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2016-08-29T17:26:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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BAPTISTA, Carlos Augusto et al. Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi. BMC Microbiology, v.15, n. 269, p. 1-10, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15503 |
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1471-2180 |
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BAPTISTA, Carlos Augusto et al. Identification of a Golgi-localized UDP-Nacetylglucosamine transporter in Trypanosoma cruzi. BMC Microbiology, v.15, n. 269, p. 1-10, 2015. 1471-2180 |
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