Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59409 |
Resumo: | University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil. |
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Santana, Vinicius C.Diniz, Mariana O.Cariri, Francisco A. M. O.Ventura, Armando M.Cunha-Neto, EdécioAlmeida, Rafael R.Campos, Marco AntonioLima, Graciela K.Ferreira, Luís C. S.2023-07-04T19:16:08Z2023-07-04T19:16:08Z2013SANTANA, Vinicius C. et al. Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8+ T Cell Responses and Protective Immunity. PLoS One, v. 8, n. 8, p. 1-10, e71322, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/5940910.1371/journal.pone.0071322engPublic Library of ScienceBicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.University of São Paulo School of Medicine. Department of Medicine. Division of Clinical Immunology and Allergy. Laboratory of Clinical Immunology and Allergy-LIM60. São Paulo, SP, Brazil.University of São Paulo School of Medicine. Department of Medicine. Division of Clinical Immunology and Allergy. Laboratory of Clinical Immunology and Allergy-LIM60. São Paulo, SP, Brazil.Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil.Millions of people worldwide are currently infected with human papillomavirus (HPV), herpes simplex virus (HSV) or human immunodeficiency virus (HIV). For this enormous contingent of people, the search for preventive and therapeutic immunological approaches represents a hope for the eradication of latent infection and/or virus-associated cancer. To date, attempts to develop vaccines against these viruses have been mainly based on a monovalent concept, in which one or more antigens of a virus are incorporated into a vaccine formulation. In the present report, we designed and tested an immunization strategy based on DNA vaccines that simultaneously encode antigens for HIV, HSV and HPV. With this purpose in mind, we tested two bicistronic DNA vaccines (pIRES I and pIRES II) that encode the HPV-16 oncoprotein E7 and the HIV protein p24 both genetically fused to the HSV-1 gD envelope protein. Mice i.m. immunized with the DNA vaccines mounted antigen-specific CD8(+) T cell responses, including in vivo cytotoxic responses, against the three antigens. Under experimental conditions, the vaccines conferred protective immunity against challenges with a vaccinia virus expressing the HIV-derived protein Gag, an HSV-1 virus strain and implantation of tumor cells expressing the HPV-16 oncoproteins. Altogether, our results show that the concept of a trivalent HIV, HSV, and HPV vaccine capable to induce CD8(+) T cell-dependent responses is feasible and may aid in the development of preventive and/or therapeutic approaches for the control of diseases associated with these viruses.Human papillomavirus (HPV)Herpes simplex virus (HSV)Human immunodeficiency virus (HIV)Bicistronic DNA VaccinesProtective ImmunityAntigen-specific CD8+ T cell responsesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALdeb_pone.0071322 1..10.pdfdeb_pone.0071322 1..10.pdfapplication/pdf1879196https://www.arca.fiocruz.br/bitstream/icict/59409/2/deb_pone.0071322%201..10.pdf7c411a5d421211f8f6a24c718fd84024MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59409/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/594092023-07-06 22:13:05.481oai:www.arca.fiocruz.br:icict/59409Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-07T01:13:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| title |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| spellingShingle |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity Santana, Vinicius C. Human papillomavirus (HPV) Herpes simplex virus (HSV) Human immunodeficiency virus (HIV) Bicistronic DNA Vaccines Protective Immunity Antigen-specific CD8+ T cell responses |
| title_short |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| title_full |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| title_fullStr |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| title_full_unstemmed |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| title_sort |
Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8(+) T Cell Responses and Protective Immunity |
| author |
Santana, Vinicius C. |
| author_facet |
Santana, Vinicius C. Diniz, Mariana O. Cariri, Francisco A. M. O. Ventura, Armando M. Cunha-Neto, Edécio Almeida, Rafael R. Campos, Marco Antonio Lima, Graciela K. Ferreira, Luís C. S. |
| author_role |
author |
| author2 |
Diniz, Mariana O. Cariri, Francisco A. M. O. Ventura, Armando M. Cunha-Neto, Edécio Almeida, Rafael R. Campos, Marco Antonio Lima, Graciela K. Ferreira, Luís C. S. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santana, Vinicius C. Diniz, Mariana O. Cariri, Francisco A. M. O. Ventura, Armando M. Cunha-Neto, Edécio Almeida, Rafael R. Campos, Marco Antonio Lima, Graciela K. Ferreira, Luís C. S. |
| dc.subject.en.none.fl_str_mv |
Human papillomavirus (HPV) Herpes simplex virus (HSV) Human immunodeficiency virus (HIV) Bicistronic DNA Vaccines Protective Immunity Antigen-specific CD8+ T cell responses |
| topic |
Human papillomavirus (HPV) Herpes simplex virus (HSV) Human immunodeficiency virus (HIV) Bicistronic DNA Vaccines Protective Immunity Antigen-specific CD8+ T cell responses |
| description |
University of São Paulo. Biomedical Sciences Institute. Department of Microbiology. São Paulo, SP, Brazil. |
| publishDate |
2013 |
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2013 |
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2023-07-04T19:16:08Z |
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2023-07-04T19:16:08Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTANA, Vinicius C. et al. Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8+ T Cell Responses and Protective Immunity. PLoS One, v. 8, n. 8, p. 1-10, e71322, 2013. |
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https://www.arca.fiocruz.br/handle/icict/59409 |
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1932-6203 |
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10.1371/journal.pone.0071322 |
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SANTANA, Vinicius C. et al. Bicistronic DNA Vaccines Simultaneously Encoding HIV, HSV and HPV Antigens Promote CD8+ T Cell Responses and Protective Immunity. PLoS One, v. 8, n. 8, p. 1-10, e71322, 2013. 1932-6203 10.1371/journal.pone.0071322 |
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