International Network for Comparison of HIV Neutralization Assays: The NeutNet Report
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15808 |
Resumo: | University of Lund. Department of Microbiology, Dermatology and Infection. Lund, Sweden. |
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Fenyö, Eva MariaHeath, AlanDispinseri, StefaniaHolmes, HarveyLusso, PaoloZolla-Pazner, SusanDonners, HelenHeyndrickx, LeoAlcami, JoseBongertz, VeraJassoy, ChristianMalnati, MauroMontefiori, DavidMoog, ChristianeMorris, LynnOsmanov, SaladinPolonis, VictoriaSattentau, QuentinSchuitemaker, HannekeSutthent, RuenpungWrin, TerriScarlatti, Gabriella2016-09-15T13:53:21Z2016-09-15T13:53:21Z2009FENYÖ, Eva Maria; et al. International Network for Comparison of HIV Neutralization Assays: The NeutNet Report. Plos One, v.4, n.2, e4505, 12p, Feb. 2009.1932-6203https://www.arca.fiocruz.br/handle/icict/1580810.1371/journal.pone.0004505engPublic Library of ScienceRede InternacionalEnsaios de neutralizaçãoHIVInternational NetworkThe Neutnet ReportHIV NeutralizationNeutralization assaysInternational Network for Comparison of HIV Neutralization Assays: The NeutNet Reportinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Lund. Department of Microbiology, Dermatology and Infection. Lund, Sweden.National Institute for Biological Standards and Control. Potters Bar, Hertfordshire, United Kingdom.San Raffaele Scientific Institute. Viral Evolution and Transmission Unit. Milan, Italy.National Institute for Biological Standards and Control. Potters Bar, Hertfordshire, United Kingdom.San Raffaele Scientific Institute. Unit of Human Virology. Milan, Italy.New York University School of Medicine. New York, NY, USA.Institute of Tropical Medicine. Department of Microbiology. Virology Unit. Antwerp, Belgium.Institute of Tropical Medicine. Department of Microbiology. Virology Unit. Antwerp, Belgium.Instituto de Salud Carlos III. Unidad de Immunopatologia del SIDA. Madrid, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.University of Leipzig. Institute of Virology. Leipzig, Germany.San Raffaele Scientific Institute. Unit of Human Virology. Milan, Italy.Duke University Medical Center. Department of Surgery. Durham, NC, USA.University Louis Pasteur. Pathogénie des infections persistantes. Strasbourg, France.National Institute for Communicable Diseases. Johannesburg, South Africa.World Health Organization. WHO-UNAIDS HIV Vaccine Initiative (IVR/HVI). Geneva, Switzerland.Henry Jackson Foundation for the Advancement of Military Medicine. Department of Vaccine Research. Rockville, Maryland, USA.University of Oxford. Sir William Dunn School of Pathology. Oxford, United Kingdom.University of Amsterdam. Academic Medical Center. Department of Experimental Immunology. Sanquin Research. Landsteiner Laboratory. Center for Infectious Diseases and Immunity Amsterdam (CINIMA). Amsterdam, Netherlands.Mahidol University. Faculty of Microbiology. Siriraj Hospital .National HIV Repository and Bioinformatic Center. Bangkok, Thailand.Monogram Biosciences Inc. South San Francisco, CA, USA.San Raffaele Scientific Institute. Viral Evolution and Transmission Unit. Milan, Italy.Background: Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays. Methods: Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at a given range of concentrations against a panel of 11 viruses representing a wide range of genetic subtypes and phenotypes. A total of 16 different assays were compared. The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus. Target cells included PBMC and genetically-engineered cell lines in either a singleor multiple-cycle infection format. Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression. Findings: PSV assays were generally more sensitive than VI assays, but there were important differences according to the virus and inhibitor used. For example, for TriMab, the mean IC50 was always lower in PSV than in VI assays. However, with 4E10 or sCD4 some viruses were neutralized with a lower IC50 in VI assays than in the PSV assays. Inter-laboratory concordance was slightly better for PSV than for VI assays with some viruses, but for other viruses agreement between laboratories was limited and depended on both the virus and the neutralizing reagent. Conclusions: The NeutNet project demonstrated clear differences in assay sensitivity that were dependent on both the neutralizing reagent and the virus. No single assay was capable of detecting the entire spectrum of neutralizing activities. Since it is not known which in vitro assay correlates with in vivo protection, a range of neutralization assays is recommended for vaccine evaluation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15808/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALvera_bongertz_etal_IOC_2012.pdfvera_bongertz_etal_IOC_2012.pdfapplication/pdf564419https://www.arca.fiocruz.br/bitstream/icict/15808/2/vera_bongertz_etal_IOC_2012.pdfb6a27b06ca31e65c79cf30ba774955dfMD52TEXTvera_bongertz_etal_IOC_2012.pdf.txtvera_bongertz_etal_IOC_2012.pdf.txtExtracted texttext/plain62648https://www.arca.fiocruz.br/bitstream/icict/15808/3/vera_bongertz_etal_IOC_2012.pdf.txt265c10de281982293816396c5de61124MD53icict/158082018-04-02 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| dc.title.pt_BR.fl_str_mv |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| title |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| spellingShingle |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report Fenyö, Eva Maria Rede Internacional Ensaios de neutralização HIV International Network The Neutnet Report HIV Neutralization Neutralization assays |
| title_short |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| title_full |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| title_fullStr |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| title_full_unstemmed |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| title_sort |
International Network for Comparison of HIV Neutralization Assays: The NeutNet Report |
| author |
Fenyö, Eva Maria |
| author_facet |
Fenyö, Eva Maria Heath, Alan Dispinseri, Stefania Holmes, Harvey Lusso, Paolo Zolla-Pazner, Susan Donners, Helen Heyndrickx, Leo Alcami, Jose Bongertz, Vera Jassoy, Christian Malnati, Mauro Montefiori, David Moog, Christiane Morris, Lynn Osmanov, Saladin Polonis, Victoria Sattentau, Quentin Schuitemaker, Hanneke Sutthent, Ruenpung Wrin, Terri Scarlatti, Gabriella |
| author_role |
author |
| author2 |
Heath, Alan Dispinseri, Stefania Holmes, Harvey Lusso, Paolo Zolla-Pazner, Susan Donners, Helen Heyndrickx, Leo Alcami, Jose Bongertz, Vera Jassoy, Christian Malnati, Mauro Montefiori, David Moog, Christiane Morris, Lynn Osmanov, Saladin Polonis, Victoria Sattentau, Quentin Schuitemaker, Hanneke Sutthent, Ruenpung Wrin, Terri Scarlatti, Gabriella |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fenyö, Eva Maria Heath, Alan Dispinseri, Stefania Holmes, Harvey Lusso, Paolo Zolla-Pazner, Susan Donners, Helen Heyndrickx, Leo Alcami, Jose Bongertz, Vera Jassoy, Christian Malnati, Mauro Montefiori, David Moog, Christiane Morris, Lynn Osmanov, Saladin Polonis, Victoria Sattentau, Quentin Schuitemaker, Hanneke Sutthent, Ruenpung Wrin, Terri Scarlatti, Gabriella |
| dc.subject.other.pt_BR.fl_str_mv |
Rede Internacional Ensaios de neutralização HIV |
| topic |
Rede Internacional Ensaios de neutralização HIV International Network The Neutnet Report HIV Neutralization Neutralization assays |
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International Network The Neutnet Report HIV Neutralization Neutralization assays |
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University of Lund. Department of Microbiology, Dermatology and Infection. Lund, Sweden. |
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2009 |
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2009 |
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2016-09-15T13:53:21Z |
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FENYÖ, Eva Maria; et al. International Network for Comparison of HIV Neutralization Assays: The NeutNet Report. Plos One, v.4, n.2, e4505, 12p, Feb. 2009. |
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10.1371/journal.pone.0004505 |
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FENYÖ, Eva Maria; et al. International Network for Comparison of HIV Neutralization Assays: The NeutNet Report. Plos One, v.4, n.2, e4505, 12p, Feb. 2009. 1932-6203 10.1371/journal.pone.0004505 |
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