Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations

Linss, Jutta Gerlinde Birggitt; Brito, Luiz Paulo; Garcia, Gabriela Azambuja; Araki, Alejandra Saori; Bruno, Rafaela Vieira; Lima, José Bento Pereira; Valle, Denise; Martins, Ademir Jesus

Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations

Detalhes bibliográficos
Autor(a) principal:	Linss, Jutta Gerlinde Birggitt
Data de Publicação:	2014
Outros Autores:	Brito, Luiz Paulo, Garcia, Gabriela Azambuja, Araki, Alejandra Saori, Bruno, Rafaela Vieira, Lima, José Bento Pereira, Valle, Denise, Martins, Ademir Jesus
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/10204
Resumo:	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.

Metadados do item

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spelling	Linss, Jutta Gerlinde BirggittBrito, Luiz PauloGarcia, Gabriela AzambujaAraki, Alejandra SaoriBruno, Rafaela VieiraLima, José Bento PereiraValle, DeniseMartins, Ademir Jesus2015-05-04T17:07:29Z2015-05-04T17:07:29Z2014LINNS, Jutta Gerlinde Birggitt et al. Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations. Parasites & Vectors, 2014, 7, n.25, 11p, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/1020410.1186/1756-3305-7-25engBioMed CentralAedesDengueControle de VetoresKdr mutationPyrethroid resistanceVector controlAedes aegyptiDengue in BrazilSodium channelAedesDengueControl de VectoresDistribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivirus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil / 4Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Background: The chemical control of the mosquito Aedes aegypti, the major vector of dengue, is being seriously threatened due to the development of pyrethroid resistance. Substitutions in the 1016 and 1534 sites of the voltage gated sodium channel (AaNaV), commonly known as kdr mutations, confer the mosquito with knockdown resistance. Our aim was to evaluate the allelic composition of natural populations of Brazilian Ae. aegypti at both kdr sites. Methods: The AaNaV IIIS6 region was cloned and sequenced from three Brazilian populations. Additionally, individual mosquitoes from 30 populations throughout the country were genotyped for 1016 and 1534 sites, based in allele-specific PCR. For individual genotypes both sites were considered as a single locus. Results: The 350 bp sequence spanning the IIIS6 region of the AaNaV gene revealed the occurrence of the kdr mutation Phe1534Cys in Brazil. Concerning the individual genotyping, beyond the susceptible wild-type (NaV S), two kdr alleles were identified: substitutions restricted to the 1534 position (NaV R1) or simultaneous substitutions in both 1016 and 1534 sites (NaV R2). A clear regional distribution pattern of these alleles was observed. The NaV R1 kdr allele occurred in all localities, while NaV R2 was more frequent in the Central and Southeastern localities. Locations that were sampled multiple times in the course of a decade revealed an increase in frequency of the kdr mutations, mainly the double mutant allele NaV R2. Recent samples also indicate that NaV R2 is spreading towards the Northern region. Conclusions: We have found that in addition to the previously reported Val1016Ile kdr mutation, the Phe1534Cys mutation also occurs in Brazil. Allelic composition at both sites was important to elucidate the actual distribution of kdr mutations throughout the country. Studies to determine gene flow and the fitness costs of these kdr alleles are underway and will be important to better understand the dynamics of Ae. aegypti pyrethroid resistance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10204/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjutta_linssetal_IOC_2014.pdfapplication/pdf1963729https://www.arca.fiocruz.br/bitstream/icict/10204/2/jutta_linssetal_IOC_2014.pdf65bdd43f2214f3c210c3b1e94dd7e4b3MD52TEXTjutta_linssetal_IOC_2014.pdf.txtjutta_linssetal_IOC_2014.pdf.txtExtracted texttext/plain44121https://www.arca.fiocruz.br/bitstream/icict/10204/3/jutta_linssetal_IOC_2014.pdf.txt5b30dceb88a82afac4c797a8010a2795MD53icict/102042022-06-24 12:19:36.849oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
title	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
spellingShingle	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations Linss, Jutta Gerlinde Birggitt Aedes Dengue Controle de Vetores Kdr mutation Pyrethroid resistance Vector control Aedes aegypti Dengue in Brazil Sodium channel Aedes Dengue Control de Vectores
title_short	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
title_full	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
title_fullStr	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
title_full_unstemmed	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
title_sort	Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations
author	Linss, Jutta Gerlinde Birggitt
author_facet	Linss, Jutta Gerlinde Birggitt Brito, Luiz Paulo Garcia, Gabriela Azambuja Araki, Alejandra Saori Bruno, Rafaela Vieira Lima, José Bento Pereira Valle, Denise Martins, Ademir Jesus
author_role	author
author2	Brito, Luiz Paulo Garcia, Gabriela Azambuja Araki, Alejandra Saori Bruno, Rafaela Vieira Lima, José Bento Pereira Valle, Denise Martins, Ademir Jesus
author2_role	author author author author author author author
dc.contributor.author.fl_str_mv	Linss, Jutta Gerlinde Birggitt Brito, Luiz Paulo Garcia, Gabriela Azambuja Araki, Alejandra Saori Bruno, Rafaela Vieira Lima, José Bento Pereira Valle, Denise Martins, Ademir Jesus
dc.subject.other.pt_BR.fl_str_mv	Aedes Dengue Controle de Vetores
topic	Aedes Dengue Controle de Vetores Kdr mutation Pyrethroid resistance Vector control Aedes aegypti Dengue in Brazil Sodium channel Aedes Dengue Control de Vectores
dc.subject.en.pt_BR.fl_str_mv	Kdr mutation Pyrethroid resistance Vector control Aedes aegypti Dengue in Brazil Sodium channel
dc.subject.es.pt_BR.fl_str_mv	Aedes Dengue Control de Vectores
description	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.
publishDate	2014
dc.date.issued.fl_str_mv	2014
dc.date.accessioned.fl_str_mv	2015-05-04T17:07:29Z
dc.date.available.fl_str_mv	2015-05-04T17:07:29Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	LINNS, Jutta Gerlinde Birggitt et al. Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations. Parasites & Vectors, 2014, 7, n.25, 11p, 2014.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/10204
dc.identifier.issn.pt_BR.fl_str_mv	1756-3305
dc.identifier.doi.pt_BR.fl_str_mv	10.1186/1756-3305-7-25
identifier_str_mv	LINNS, Jutta Gerlinde Birggitt et al. Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations. Parasites & Vectors, 2014, 7, n.25, 11p, 2014. 1756-3305 10.1186/1756-3305-7-25
url	https://www.arca.fiocruz.br/handle/icict/10204
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
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Distribution and dissemination of the Val1016Ile and Phe1534Cys Kdr mutations in Aedes aegypti Brazilian natural populations

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